A rapid direct radioimmunoassay for type C virus group-specific antigen and antibody

Oroszlan, Stephen; White, Martin M.H.; Gilden, Raymond V.; Charman, Howard P.

doi:10.1016/0042-6822(72)90376-5

Cited by 52 publications

(25 citation statements)

References 5 publications

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“…Our results obtained with radioimmunoassay support the presence of anti-gs-a antibodies in the sera of chickens carrying RSV-induced tumors [14]. Studies along these lines are now in progress in our laboratory as well as in other laboratories, which have recently developed radioimmunoassays for the major gs antigens of mammalian RNA tumor viruses [15,16].…”

Section: Discussionsupporting

confidence: 54%

Radioimmunoassay of Avian RNA Tumor Virus Group-Specific Antigen

Suni¹,

Vaheri²,

Ruoslahti³

1973

Intervirology

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Section: Discussionsupporting

confidence: 54%

Radioimmunoassay of Avian RNA Tumor Virus Group-Specific Antigen

Suni¹,

Vaheri²,

Ruoslahti³

1973

Intervirology

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“…Since that time several groups have described RIAs for purified core or structural virus proteins (6)(7)(8)(9)(10)(11)(12)(13)(14). This approach does have certain advantages.…”

Section: Discussionmentioning

confidence: 99%

“…Since then there has been increasing interest in the application of this methodology in a variety of investigations in virology. Most of these have been concerned with the development of sensitive radioimmunoassays for the protein components of RNA C-type oncogenic viruses, i.e., the core proteins from mammalian (6)(7)(8)(9)(10)(11)(12)(13) and avian species (14) (generally less than 30,000 daltons) and mammalian membrane glycopeptides (about 70,000 daltons) (13), which appear to be elements of the viral envelope. Some (15,16) have been developed for the assay of intact mouse mammary tumor virus.…”

mentioning

confidence: 99%

Radioimmunoassay for intact Gross mouse leukemia virus.

Yalow¹,

Gross²

1976

Proc. Natl. Acad. Sci. U.S.A.

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A radioimmunoassay for intact Gross leukemia virus has been developed using 2RI-labeled Gross virus grown in tissue culture and guinea pig antisera to Gross virus grown either in tissue culture or harvested from leukemic C3H(f) mice. Separation of bound from free labeled virus was effected using the double antibody method. The assay can detect fewer than 108 virus particles and has been used to measure the viral content of individual organs from inoculated leukemic C3H(t) mice and from Ak mice with spontaneous leukemia. Organs from noninoculated healthy C3H(f) mice crossreacted poorly in the system, virus generally being detectable only in the thymus-and spleen and at low concentration. In some of the inoculated C3H(f) leukemic mice the viral content of as little as 0.5 p1 of plasma is measurable. That this assay is for intact virus and not for soluble antigens of the viral envelope was proven by the observation that the immunoreactive material of plasma and extracts from thymus and liver of leukemic mice has a buoyant density in sucrose of 1.17-1.18 g/ml, corresponding to that of intact virus grown in tissue culture. With this sensitivity it may now be possible to quantitate viral concentrations in tissue and body fluids from the time of inoculation through the development of obvious pathology.Since its application to the measurement of plasma insulin in man (1, 2), radioimmunoassay (RIA) has increasingly become the method of choice for the determination of the concentration of peptidal and nonpeptidal hormones and nonhormonal substances of medical and biological interest in plasma and tissue (see ref. 3 for review). RIA was applied to a viral antigen for the determination of circulating hepatitis B antigen and its antibody (4, 5). Since then there has been increasing interest in the application of this methodology in a variety of investigations in virology. Most of these have been concerned with the development of sensitive radioimmunoassays for the protein components of RNA C-type oncogenic viruses, i.e., the core proteins from mammalian (6-13) and avian species (14) (generally less than 30,000 daltons) and mammalian membrane glycopeptides (about 70,000 daltons) (13), which appear to be elements of the viral envelope. Some (15, 16) have been developed for the assay of intact mouse mammary tumor virus. In this report we describe an RIA for intact Gross murine leukemia virus and its application to the measurement of the virus content of plasma and individual organs of normal, noninoculated, nonleukemic C3H(f) mice and of inoculated leukemic C3H(f) mice and of Ak mice with spontaneous leukemia. MATERIALS AND METHODS AnimalsMice of the C3H(f) inbred line raised by brother-to-sister mating in our laboratory were employed. Ak mice from our colony were also studied after the development of spontaneous leukemia. Rats of the Sprague-Dawley strain, bred in our laboratory by brother-to-sister mating, were used for serial propagation of the Gross mouse leukemia virus adapted to rats.

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“…Interspecies crossreactivity was initially shown for several early type C virus isolates (9,10). The advent of radioimmunologic techniques (11,12) made it possible to demonstrate the presence of interspecies determinants common to the major structural protein (p3Os) of all known mammalian type C viruses (13)(14)(15). Similar conclusions have been drawn from partial sequence analysis of diverse viral p30s (16).…”

Section: Abs Ractmentioning

confidence: 99%

Major structural proteins of type B, type C, and type D oncoviruses share interspecies antigenic determinants.

Barbacid¹,

Long²,

Aaronson³

1980

Proc. Natl. Acad. Sci. U.S.A.

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Interspecies antigenic determinants have been used as markers to study the evolution of oncoviruses. By radioimmunologic techniques, it has been possible to detect crossreactivities among the major structural proteins of prototype viruses representing each of the three major Oncovirinae genera. Two different subsets of interspecies antigenic determinants were demonstrated. One encompasses known type B and type D viruses; the other is shared by type D and mammalian type C viruses. Such determinants were also demonstrated in certain as yet unclassified oncoviruses. These findings provide experimental evidence supporting the concept of an evolutionary relationship among the three major oncovirus genera.

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A rapid direct radioimmunoassay for type C virus group-specific antigen and antibody

Cited by 52 publications

References 5 publications

Radioimmunoassay of Avian RNA Tumor Virus Group-Specific Antigen

Radioimmunoassay of Avian RNA Tumor Virus Group-Specific Antigen

Radioimmunoassay for intact Gross mouse leukemia virus.

Major structural proteins of type B, type C, and type D oncoviruses share interspecies antigenic determinants.

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