A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

Song, Dandan; Qu, Xiaofeng; Liu, Yu‐Shen; Li, Li; Yin, Di; Li, Juan; Xu, Kun; Xie, Rui-Hua; Zhai, Yue; Zhang, Huiwen; Bao, Hanying; Zhao, Chao; Wang, Juan; Song, Xiaohong; Song, Wenzhi

doi:10.1186/s11671-017-1941-z

Cited by 32 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sun et al detected S. pullorum by polyclonal antibody and blue-silica nanoparticles ranging from 4.4 Â 10 2 to 4.4 Â 10 7 cfu mL À1 [14], and also detected S. pullorum and S. gallinarum by silica and magnetic nanoparticles in milk powder 8.8 Â 10 2 cfu mL À1 [16]. Song et al detected Brucella by using polyclonal antibody-conjugating quantum dots and antibody modified magnetic beads, 10 2 -10 5 cfu mL À1 [28]. Wu et al determined B. melitensis by sensitivity method and using gold nanoparticles-screen-printed carbon electrode 1 Â 10 4 to 4 Â 10 5 cfu mL À1 [29].…”

Section: Discussionmentioning

confidence: 99%

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Shams

Zarif

2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Brucellosis has always been a threat to the health and economics of societies. We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibody against B. abortus, which was activated by 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form detection and capture probes, respectively. After adding the conjugates to the bacterial suspension, sandwich structure of PMNPs B. abortus-blue-SiNPs was formed and then separated by a magnet. The blue dye was released from the silica structure and its absorbance was measured at 670 nm with a spectrophotometer. Under optimal conditions, results showed a wide dynamic range from 1.5 Â 10 3 to 1.5 Â 10 8 cfu mL À1 with a detection limit of 450 cfu mL À1. The specificity of the sensor was confirmed in comparison with 5 other bacteria. Also, during the 120-days period, the complex was stable. The results suggested that it can be used in real samples (R 2 ¼ .9865). This designed colorimetric immunoassay strategy can be used as an alternative, user-friendly and on-site tool for the rapid detection of Brucella spp. compared to other common methods with high sensitivity and specificity in a short time.

show abstract

Section: Discussionmentioning

confidence: 99%

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Shams

Zarif

2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…QD immunochromatographic test strips have strict requirements for the immunogenicity of labeling and coating proteins. Dan et al developed a detection of Brucella with QDs and magnetic beads conjugating with different polyclonal antibodies and this method takes 105 min with a detection limit of 10 3 CFU/mL [43]. The method we established only need 10-15 min to obtain nal results with a limit of 1.05 ng/mL.…”

Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The detection results were achieved by using the ratio of the fluorescence signals of the test and control lines (HT/HC) with a threshold of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseases was observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

show abstract

“…Dan et al developed a method to detect Brucella by combining QD and magnetic beads with different polyclonal antibodies. The method requires 105 min and the limit of detection was 10 3 CFU/mL [ 43 ]. The established method in this study takes only 10–15 min to obtain the test results and its limit of detection is 1.05 ng/mL.…”

Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2021

BMC Vet Res

View full text Add to dashboard Cite

Background Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum. Results The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The critical threshold for determining negative or positive through the ratio of the fluorescent signal of the test line and the control line (HT / HC) is 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICST and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, the sensitivity of RBPT is 1:32, and no cross reaction with the sera of other related diseases was observed. Conclusion In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

show abstract

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

Cited by 32 publications

References 27 publications

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Contact Info

Product

Resources

About