A rapid colloidal gold immunochromatographic assay for the diagnosis of coronavirus disease 2019

Wang, Xiaoling; Wang, Lei; Hasi, Chaolu; Wang, Yu-Po; Khan, Ajab; Ren, Binzhi; Liu, Zhizhen; Hou, Shun-Lin; Yang, Lihong; Zhang, Liaoyun; Dong, Yongkang; Xu, Jun; Xie, Jun

doi:10.1097/cm9.0000000000000922

Cited by 6 publications

(8 citation statements)

References 5 publications

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“…Colloidal gold was synthesized using a one‐step reduction method in which trisodium citrate reduces HAuCl 4 13 . The basic principle is to add a certain amount of reducing agent to a gold solution of a certain concentration to make gold ions into gold atoms 14 .…”

Section: Methodsmentioning

confidence: 99%

“…It was then air-dried at 37 • C for 2 h in an air-blast drying oven and stored at room temperature sealed in a dried aluminum foil bag for future use. 13 The conjugate pad was prepared by fixing the colloidal gold coupling of anti-ELABELA mAb on the polyester fiber, which was treated in the same way as the sample pad. Then, anti-ELABELA mAb wirecolloidal gold coupling was sprayed onto the polyester fiber with a volume of 20 μL/cm through a Biodot-xyz3060 colloidal gold strip 3D gold spray film stripper (United States/BIODOT).…”

Section: Preparation and Assembly Of Colloidal Gold Test Stripmentioning

confidence: 99%

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Predicting late‐onset preeclampsia by detecting ELABELA content using an immunochromatographic colloidal gold test strip

Yang,

Liu,

Zhang

et al. 2023

J of Clinical Hypertension

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Preeclampsia is a pregnancy disorder that seriously affects the outcome of mothers and infants and lacks effective prediction and diagnosis methods. ELABELA is the second endogenous ligand of the apelin receptor (APJ) and is associated with the pathogenesis of preeclampsia. In a previous study, the authors found that the downregulation of ELABELA expression is closely related to late‐onset preeclampsia, which may be a marker for the clinical diagnosis of late‐onset preeclampsia. In this study, the authors again collected 120 maternal blood samples, including 60 pregnant women with a medical diagnosis of late‐onset preeclampsia. ELISA results showed that the serum ELABELA concentration in late‐onset preeclampsia pregnant women (12.57 ± 7.77 ng/mL) was significantly lower than that in normal pregnant women (36.99 ± 23.58 ng/mL), which was consistent with previously reported results. Therefore, the authors used an ELABELA monoclonal antibody to label four colloidal gold nanoparticles with different diameters (15, 30, 55, and 150 nm) and developed a transverse‐flow immunochromatographic band for the rapid and accurate detection of serum ELABELA levels. The strip test shows that colloidal gold with a diameter of 30 nm can be used as a good ELABELA detection marker and had more than 90% positive detection effect. Therefore, the authors hope that the colloidal gold strip with ELABELA as the diagnostic index developed by us will be popularized and applied in clinical diagnosis.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation and Assembly Of Colloidal Gold Test Stripmentioning

confidence: 99%

Predicting late‐onset preeclampsia by detecting ELABELA content using an immunochromatographic colloidal gold test strip

Yang,

Liu,

Zhang

et al. 2023

J of Clinical Hypertension

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“…Paper has become a simple and reliable platform for analytical instruments, and one of the most well-known applications is the lateral flow immunoassay (LFA). Due to its unique advantages, including rapidity, portability, and simplicity, LFAs have been widely used as tests for clinical diagnosis [ 15 , 16 ], infectious diseases [ 17 ], and the detection of chemical contaminants [ 18 ]. LFAs combine immune-specific recognition and sensitive nano-signal characteristics, creating an excellent tool for use in point-of-care tests (POCT) for food safety.…”

Section: Introductionmentioning

confidence: 99%

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

et al. 2022

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The illegal use of β-adrenergic agonists during livestock growth poses a threat to public health; the long-term intake of this medication can cause serious physiological side effects and even death. Therefore, rapid detection methods for β-adrenergic agonist residues on-site are required. Traditional detection methods such as liquid chromatography have limitations in terms of expensive instruments and complex operations. In contrast, paper methods are low cost, ubiquitous, and portable, which has led to them becoming the preferred detection method in recent years. Various paper-based fluidic devices have been developed to detect β-adrenergic agonist residues, including lateral flow immunoassays (LFAs) and microfluidic paper-based analytical devices (μPADs). In this review, the application of LFAs for the detection of β-agonists is summarized comprehensively, focusing on the latest advances in novel labeling and detection strategies. The use of μPADs as an analytical platform has attracted interest over the past decade due to their unique advantages and application for detecting β-adrenergic agonists, which are introduced here. Vertical flow immunoassays are also discussed for their shorter assay time and stronger multiplexing capabilities compared with LFAs. Furthermore, the development direction and prospects for the commercialization of paper-based devices are considered, shedding light on the development of point-of-care testing devices for β-adrenergic agonist residue detection.

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“…The dimension of viruses is commonly in the range of nano-scale and hence, the nanomedicine field is heading to investigate the nanoparticle uptake in cells, and thus the approaches and values from the synthesis of nanoparticles with cells present the mechanisms that are before viral infections [ 11 ]. Gold nanoparticles immunochromatographic [ 12 ] and electrochemical tests have been under development as a rapid and cost-effective screening method for viral infection detection [ 13 ]. The application of silver nanomaterials (AgNMs) has been regarded as a new approach against resistant viral and bacterial strains [ 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

Nanobiocide Based-Silver Nanomaterials Upon Coronaviruses: Approaches for Preventing Viral Infections

2021

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The effectiveness of silver nanomaterials (AgNMs), as antiviral agents, has been confirmed in humans against many different types of viruses. Nanobiocides-based AgNMs can be effectively applied to eliminate coronaviruses (CoVs), as the cause of various diseases in animals and humans, particularly the fatal human respiratory infections. Mostly, these NMs act effectively against CoVs, thanks to the NMs’ fundamental anti-viral structures like reactive oxygen species (ROS), and photo-dynamic and photo-thermal abilities. Particularly, the antiviral activity of AgNMs is clarified under three inhibitory mechanisms including viral entry limitation, attachment inhibition, and viral replication limitation. It is believed that nanobiocide with other possible materials such as TiO2, silica and, carbon NMs exclusively nano-graphene materials can emerge as a more effective disinfectant for long-term stability with low toxicity than common disinfectants. Nanobiocides also can be applied for the prevention and treatment of viral infections specifically against COVID-19. Graphic Abstract

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A rapid colloidal gold immunochromatographic assay for the diagnosis of coronavirus disease 2019

Cited by 6 publications

References 5 publications

Predicting late‐onset preeclampsia by detecting ELABELA content using an immunochromatographic colloidal gold test strip

Predicting late‐onset preeclampsia by detecting ELABELA content using an immunochromatographic colloidal gold test strip

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

Nanobiocide Based-Silver Nanomaterials Upon Coronaviruses: Approaches for Preventing Viral Infections

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