A rapid approach to prepare poly(2-methyl-2-oxazoline)-based antifouling coating by UV irradiation

Zhu, Haikun; Mumtaz, Fatima; Zhang, Chong; Tan, Lin; Liu, Songtao; Zhang, Yalin; Pan, Chao; Wang, Yanmei

doi:10.1016/j.apsusc.2017.07.260

Cited by 25 publications

(10 citation statements)

References 45 publications

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“…In both studies with PMeOx‐poly(4‐vinyl pyridine) or PEI‐ g ‐PMeOx, the coated substrates were found to have low bovine serum albumin, platelet, and human umbilical vein endothelial cells (HUVEC) adhesion up to 28 days in vitro. Similar results were recently reported by the same group for brush copolymers of PMeOx‐poly(4‐vinyl pyridine) synthesized by reversible addition–fragmentation chain transfer polymerization of a PMeOx–methacrylate macromonomer synthesized by capping the CROP of MeOx with methacrylic acid …”

Section: Emerging Applicationssupporting

confidence: 89%

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Dargaville

Park

Hoogenboom

2018

Macromolecular Bioscience

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The synthesis of poly(2-oxazoline)s has been known since the 1960s. In the last two decades, they have risen in popularity thanks to improvements in their synthesis and the realization of their potential in the biomedical field due to their "stealth" properties, stimuli responsiveness, and tailorable properties. Even though the bulk of the research to date has been on linear forms of the polymer, they are also of interest for creating network structures due to the relatively easy introduction of reactive functional groups during synthesis that can be cross-linked under a variety of conditions. This opinion article briefly reviews the history of poly(2-oxazoline)s and examines the in vivo data on soluble poly(2-oxazoline)s to date in an effort to predict how hydrogels may perform as implantable materials. This is followed by an overview of the most recent hydrogel synthesis methods and emerging applications, and is concluded with a section on the future directions predicted for these fascinating yet underutilized polymers.

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Section: Emerging Applicationssupporting

confidence: 89%

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Dargaville

Park

Hoogenboom

2018

Macromolecular Bioscience

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“…Lind et al 35 immobilized poly(2-oxazoline)s onto an azide-modified surface by "click" reaction. Additionally, surface-initiated polymerization, 36 photo-immobilization, 37 and self-assembled monolayers 38 have also been applied to construct poly(2-oxazoline)sfunctionalized surfaces. Among those methods, solvent evaporation and surface termination are relatively simple and practical.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Wang

Ren

Bernaerts

et al. 2020

ACS Appl. Bio Mater.

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As one of the nonenzymatic cell-harvesting technologies, a thermal-responsive surface based on poly(2-oxazoline)s has achieved initial success in supporting the adhesion and thermal-induced detachment of animal cells. However, because of the laborious preparation procedure, this technique was only limited to research purposes. In this work, through using poly(glycidyl methacrylate) (PGMA) as the anchor layer, poly(2-propyl-2-oxazoline)s (PPOx) were grafted onto glass wafers through a facile two-step coating and annealing procedure for nonenzymatic cell harvesting. In the first step, the piranha solution-activated glass wafers were immersed into the chloroform solution of PGMA and then annealed for a given period of time to immobilize PGMA onto the glass wafers through the bonding between epoxy groups and hydroxyl groups. In the second step, the PGMA-coated glass wafers were further immersed into the chloroform solution of carboxyl-functionalized PPOx. After annealing, PPOx were immobilized onto the PGMA layer through the bonding between carboxyl groups and the residual epoxy groups. Atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry were used to characterize the modified glass wafers. The results of cytocompatibility evaluation showed that the PPOx-coated glass wafers were almost nontoxic and were able to support the adhesion and proliferation of L929 cells well. By lowering the temperature to 8 °C, L929 and Vero cells were successfully detached from the PPOx-coated glass wafers without any enzymatic treatment. Further cultivation has demonstrated that the cooling procedure had little effect on cell viability, and the cells still retained good viability after harvesting.

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“…By this method, glass, silicon, gold and polydimethylsiloxane (PDMS) surfaces were efficiently protected against contamination by bovine serum albumin (BSA) and human umbilical vein endothelial cells (HUVECs). [ 24 ]…”

Section: Surface Functionalization Strategiesmentioning

confidence: 99%

Fabrication of Biopassive Surfaces Using Poly(2‐alkyl‐2‐oxazoline)s: Recent Progresses and Applications

Trachsel

Romio

Ramakrishna

et al. 2020

Adv Materials Inter

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monomer composition enables to obtain hydrophilic and bioinert and/or amphiphilic PAOXA species displaying thermoresponsive properties. [6] Simultaneously, careful choice of initiator and/or terminator agents gives access to end-functional or heterobifunctional polymers, which can be directly applied for surface modification, or used as building blocks to yield structurally more complex adsorbates targeting specific substrates or generating well-defined polymer architectures at the interface. [7-9] Among the most recent studies focusing on the fabrication of biopassive PAOXA films, most of them involved grafting of pre-synthesized adsorbates by exploiting functional anchors that are specific for the target substrate to passivate. In other works, the growth of a PAOXAbased film was triggered from the substrate itself, through surface-initiated CROP (SI-CROP), while interest has been rising in applying plasma polymerization of 2-oxazolines to generate robustly attached coatings on an array of different supports. Through these strategies, and with the general objective of suppressing nonspecific interaction with biological entities, a broad range of biomaterials, ranging from inorganic supports, plastics and nanomaterials of diverse composition have been successfully functionalized with PAOXAs. While during the first decade of 2000s efforts were spent in dissecting the fundamental properties of PAOXAs assemblies on surfaces, [3,10,11] during the past five years their application in different coating formulations for biomaterials has been progressively expanding, and at least some of them are probably mature for a direct involvement of industry. In this progress report, we critically review the most recent studies focusing on the fabrication of PAOXA coatings, especially concentrating on the generation of bioinert surfaces, which probably represents their most appealing and technologically relevant application. 2. Composition of Bioinert PAOXA-Based Surfaces Among the different PAOXA species that were applied on surfaces to generate coatings, hydrophilic poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) showed the most pronounced inertness towards nonspecific interactions with serum proteins, cells and bacteria. A recent study by Morgese et al. identified the superior biopassivity of linear Poly(2-alkyl-2-oxazoline)s (PAOXAs) are emerging among the most promising nonionic alternatives to poly(ethylene glycol)s (PEGs), specifically in the modification and functionalization of biomaterials. Due to their chemical tailorability and robustness, coupled to their relatively easy synthesis, PAOXAs are increasingly applied as adsorbates to generate bioinert surfaces that prevent nonspecific contamination by proteins, cells and bacteria. Passivation of medical devices, sensors and cell-sensitive platforms with PAOXAs enables a nearly quantitative suppression of nonspecific biological contamination, while biopassivity is maintained over longer incubation times than those recorded for more degradable PEG-b...

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A rapid approach to prepare poly(2-methyl-2-oxazoline)-based antifouling coating by UV irradiation

Cited by 25 publications

References 45 publications

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Fabrication of Biopassive Surfaces Using Poly(2‐alkyl‐2‐oxazoline)s: Recent Progresses and Applications

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