A rapid approach to investigate spatiotemporal distribution of phytohormones in rice

Cai, Wen-Jing; Ye, Tian-Tian; Wang, Qing; Cai, Bingna; Feng, Yu‐Qi

doi:10.1186/s13007-016-0147-1

Cited by 39 publications

(33 citation statements)

References 36 publications

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“…Liquid chromatography coupled with mass spectrometry (LC-MS) [117,[132][133][134][135] has become much more widely used because of its high capacity to separate and detect plant hormones without derivatization but with high sensitivity and efficiency [71]. Ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) is now one of the most common approaches for quantification of auxins and their conjugates [136][137][138][139][140], often employing triple quadrupole mass spectrometers that improve specificity and reduce detection limits [136][137][138][139]. Recent improvements in GC-MS/MS and LC-MS/MS techniques for auxin analysis have been the focus of comprehensive reviews [9,135].…”

Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.

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Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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“…To date, MS studies have identified many plant hormones and their metabolites that might be involved in the molecular mechanisms and physiological functions of plant development, but the analysis at unicellular level has remained a challenge. Several large-scale studies on plant hormone have been reported, these studies have promoted our understanding on the dynamic spatial-temporal distribution of plant hormone [19,35,77]. Current sample pretreatment methods for plant hormone is expected to be further optimized for realizing the in situ, real-time and high spatial resolution.…”

Section: Discussionmentioning

confidence: 99%

“…1) [62]. Cai et al [77] developed a clean-up strategy employing a single-step dispersive solid-phase extraction (DSPE) combined with UPLC-MS/MS to obtain spatialtemporal information of 54 plant hormones including auxins, ABA, SA, JA, GAs and CKs. Wang et al [75] used a combination of EME and LC-MS/MS to quantitatively detect six acidic plant hormones in 20 mg citrus leaf sample with a limit of detection (LOD) ranging from 0.1 to 10 ng mL −1 .…”

Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

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“…Just a small number of GAs have intrinsic biological activity, and they regulate many aspects of growth and development throughout the plant life cycle, from seed germination and vegetative growth to reproductive growth (Swain et al ., ; Eriksson et al ., ; Gomez et al ., ; Racca et al ., ). The level and distribution of GAs in plant tissues are dynamically changing with different plant growth and development stages as well as external living environments (Vettakkorumakankav et al ., ; Cai et al ., ). To gain a deep insight into their physiological functions and action mechanisms, it is crucial to know the precise location of endogenous GAs and their local concentrations (Novak et al ., ).…”

Section: Introductionmentioning

confidence: 97%

One‐pot sample preparation approach for profiling spatial distribution of gibberellins in a single shoot of germinating cereal seeds

Liu

et al. 2019

The Plant Journal

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Summary Sample preparation remains a bottleneck in the rapid and reliable quantification of gibberellins (GAs) for obtaining an insight into the physiological processes mediated by GAs. The challenges arise from not only the extremely low content of GAs in complex plant matrices, but the poor detectability of GAs by mass spectrometry (MS) in negative ion mode. In an effort to solve these urgent difficulties, we present a spatial‐resolved analysis method to investigate the distribution of GAs in tiny plant tissues based on a simplified one‐pot sample preparation approach coupled with ultrahigh‐performance liquid chromatography‐tandem MS. By integrating extraction and derivatization into one step, target GAs were effectively extracted from plant materials and simultaneously reacted with N‐(3‐dimethylaminopropyl)‐N′‐ethylcarbodiimide, the sample preparation time was largely shortened, the probability of sample loss was minimized and the detection sensitivity of MS was also greatly improved compared with underivatized GAs. Under optimal conditions, the method was validated from the quantification linearity, limits of detection and limits of quantification in the presence of plant matrices, recoveries, and precision. With the proposed method, 15 endogenous GAs were detected and, among these, 11 GAs could be quantified in 0.50 mg fresh weight (FW) wheat shoot samples, and five GAs were quantified in only 0.15 mg FW developing seed samples of Arabidopsis thaliana. The distribution patterns of GAs along both the non‐13‐hydroxylation pathway and the early 13‐hydroxylation pathway in a single shoot of germinating wheat, rice and maize seeds were finally profiled with a spatial resolution down to approximately 1 mm2.

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A rapid approach to investigate spatiotemporal distribution of phytohormones in rice

Cited by 39 publications

References 36 publications

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

One‐pot sample preparation approach for profiling spatial distribution of gibberellins in a single shoot of germinating cereal seeds

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