A rapid and stable ITLC procedure for the determination of the radiochemical purity of 99Tcm-tetrofosmin

Abstract

99Tcm-tetrofosmin (Myoview, Amersham Healthcare) is widely used as a radiopharmaceutical for myocardial perfusion imaging. Control of its radiochemical purity after reconstitution is usually performed by means of ITLC-SG paper chromatography in a mobile phase of methylene chloride/acetone (65/35), as recommended by the manufacturer. The present study describes the application of tetrahydrofuran in phosphate buffer for the development of 99Tcm-tetrofosmin on ITLC-SG strips. The use of this mobile phase significantly improves the separation between labelled tetrofosmin and unbound pertechnetate. The time for development is about 1 min and the solvent is stable for at least 1 year. In addition, the volume spotted on the strip does not affect the migration of 99Tcm-tetrofosmin. The labelling efficiency of 99Tcm-tetrofosmin can successfully be monitored by means of this method as a daily routine procedure.

Preparation, radiochemical purity control and stability of99mTc-mertiatide (Mag-3)

“…Since the HPLC system in use has been especially designed for the quantification of weak β-emitters like 3 H and 14 C, we first assess the behavior of 99m Tc in a liquid scintillation detection system. Under conditions of low-quenching (i.e.…”