A rapid and simple resazurin assay to detect minimum inhibitory concentrations of first-line drugs for Mycobacterium tuberculosis isolated from cerebrospinal fluid

Kumar, Kavitha; Giribhattanavar, Prashant; Sagar, Chandrasekhar; Patil, Shripad A.

doi:10.1016/j.jgar.2017.09.012

Cited by 11 publications

(7 citation statements)

References 15 publications

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“…Explant cell metabolism was assessed via the resazurin reduction assay as previously described. 40,42 Following a 3-hour incubation in the medium, intensity of the reduced product, resorufin, was measured at 530/590nm. Values were then normalized to daily control wells with no explants, such that a value below 1 in presented data would represent a culture well with no living tissue.…”

Section: Explant Cell Viability Cell Density and Cell Metabolismmentioning

confidence: 99%

Age-associated changes in the response of tendon explants to stress deprivation is sex-dependent

Connizzo

Piet

Shefelbine

et al. 2019

Connective Tissue Research

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The incidence of tendon injuries increases dramatically with age, which presents a major clinical burden. While previous studies have sought to identify age-related changes in extracellular matrix structure and function, few have been able to explain fully why aged tissues are more prone to degeneration and injury. In addition, recent studies have also demonstrated that age-related processes in humans may be sex-dependent, which could be responsible for muddled conclusions in changes with age. In this study, we investigate short-term responses through an ex vivo explant culture model of stress deprivation that specifically questions how age and sex differentially affect the ability of tendons to respond to altered mechanical stimulus. We subjected murine flexor explants from young (4 months of age) and aged (22-24 months of age) male and female mice to stress-deprived culture conditions for up to one week and investigated changes in viability, cell metabolism and proliferation, matrix biosynthesis and composition, gene expression, and inflammatory responses throughout the culture period. We found that aging did have a significant influence on the response to stress deprivation, demonstrating that aged explants have a less robust response overall with reduced metabolic activity, viability, proliferation, and biosynthesis. However, age-related changes appeared to be sex-dependent. Together, this work demonstrates that the aging process and the subsequent effect of age on the ability of tendons to respond to stressdeprivation are inherently different based on sex, where male explants favor increased activity,

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Section: Explant Cell Viability Cell Density and Cell Metabolismmentioning

confidence: 99%

Age-associated changes in the response of tendon explants to stress deprivation is sex-dependent

Connizzo

Piet

Shefelbine

et al. 2019

Connective Tissue Research

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“…The result was recorded after 20 h at 37 • C for the bacterial strains and 72 h at 30 • C for the yeast strain. This experiment was visually observed and further confirmed using 30 µl of 0.01% resazurin (HiMedia) solution [73] after incubating the plates for 3 h. The result was considered as positive by observing the changing of the well's color to pink. MBC/MFC values of the CS and the ZnNPs-C-CS, MnNPs-C-CS and SnNPs-C-CS were obtained by taking a sample (10 µL) from the wells (before resazurin indicator) displaying no distinguishable microbial growth onto their specific agar media (MHA, SDA plates) [74].…”

Section: Minimum Inhibitory Concentration and Minimum Bactericidal Or Fungicidal Concentrationsmentioning

confidence: 76%

Enhancement of A Cationic Surfactant by Capping Nanoparticles: Synthesis, Characterization and Multiple Applications

et al. 2020

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There is scarce information on cationic surfactants’ biocidal and corrosion inhbibition effects on Slime-Forming Bacteria (SFB) isolated from oil field formation water. Therefore, this work focused on the the synthesis of a cationic surfactant (CS) to increase its features by capping different metal nanoparticles (zinc, ZnNPs-C-CS; manganese, MnNPs-C-CS and tin, SnNPs-C-CS) and used them as biocides and corrosion inhibitors. The cationic surfactant was synthesized and characterized by Fourier-Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Afterwards, different nanoparticles were synthesized, characterized, and exploited to cap by the CS. The CS and the different nanoparticles capped by the CS were tested for their antimicrobial susceptibility against standard bacterial and yeast strains. The synthesized compounds were further evaluated as anti-biofilms agents against positively-developed bacterial biofilms. Moreover, the CS and the ZnNPs-C-CS, MnNPs-C-CS, and SnNPs-C-CS were assessed as potential biocides against SFB, particularly Pseudomonas sp. (isolated from contaminated formation water), and as corrosion inhibitors against cultivated salinity. The results revealed the great effect of the different CS-capped NPs as broad-spectrum antimicrobial and anti-biofilm agents at lower Minimum Inhibitory Concentrations (MICs), Minimum Bactericidal Concentrations (MBCs), Minimum Fungicidal Concentrations (MFCs) and Minimum Biofilm Inhibitory Concentrations (MBICs), and the activities were reported in order of SnNPs-C-CS > MnNPs-C-CS > ZnNPs-C-CS > CS. Furthermore, the ZnNPs-C-CS, MnNPs-C-CS, and SnNPs-C-CS demonstrated biocidal and corrosion inhibition effects against Pseudomonas sp. at a salinity of 3.5% NaCl, with metal corrosion inhibition efficiencies of 88.6, 94.0 and 96.9%, in comparison to a CS efficiency of 85.7%. In conclusion, the present work provides a newly synthesized cationic surfactant and has enhanced its antimicrobial and its metal corrosion inhibition effects by capping different nanoparticles, and it has been successfully applied against slime-forming bacteria at a salinity of 3.5% NaCl.

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“…To observe and confirm the results, 30 μL of 0.015% resazurin (Hi Media) solution as an oxidation-reduction indicator was added to all wells (Kumar et al, 2018), and the plates were further incubated for at least 2 h. A well with no color change indicated a negative result or no bacterial or candida growth. The results were positive if there was a change the well's color to pink, which indicates bacterial and candida growth.…”

Section: Antimicrobial Susceptibility Test Of the Synthesized Fhpao mentioning

confidence: 99%

Novel Gemini Cationic Surfactants: Thermodynamic, Antimicrobial Susceptibility, and Corrosion Inhibition Behavior against Acidithiobacillus ferrooxidans

Labena

Hamed

Ismael

et al. 2020

J Surfact & Detergents

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The Egyptian oil and gas industry is suffering from severe metal corrosion problems, particularly microbial‐induced corrosion. There is limited knowledge on the corrosion inhibition of carbon steels in the presence of an acidophilic, iron‐oxidizing bacterial species Acidithiobacillus ferrooxidans. Therefore, in this study, novel Gemini cationic surfactants, in three forms depending on variation in alkyl chains of 8, 12, and 16 carbon atoms named FHPAO, FHPAD, and FHPAH, respectively, were synthesized and characterized by Fourier transform infrared and nuclear magnetic resonance spectroscopy. The surface parameters and the thermodynamic of the synthesized surfactants were evaluated at three different temperatures, 20, 40, and 60 °C. The synthesized Gemini cationic surfactants were tested as broad‐spectrum antimicrobial, antibacterial and anticandida agents. They evaluated as biocides and corrosion inhibitors against Acidithiobacillus ferrooxidans. FHPAD showed higher adsorption ability at the solution interface and higher affinity to construct micelles than FHPAO and FHPAH. Both adsorption and micellization processes were hydrophobic and temperature dependent. FHPAO, FHPAD and FHPAH exhibited wide‐spectrum antimicrobial activities, and the highest activity and the lowest minimum bactericidal/fungicidal inhibitory concentrations were attributed to FHPAD. Furthermore, synthesized FHPAD demonstrated the highest metal corrosion inhibition efficiency of 95.5% at 5 mM in comparison to 87.5% and 81.7% for FHPAO and FHPAH, respectively. In conclusion, this study provides novel synthesized cationic surfactants with many applications in the oil and gas industry, such as broad‐spectrum antimicrobial, biocides, and corrosion inhibitors for acidophilic, iron‐oxidizing bacterial species Acidithiobacillus ferrooxidans.

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A rapid and simple resazurin assay to detect minimum inhibitory concentrations of first-line drugs for Mycobacterium tuberculosis isolated from cerebrospinal fluid

Cited by 11 publications

References 15 publications

Age-associated changes in the response of tendon explants to stress deprivation is sex-dependent

Age-associated changes in the response of tendon explants to stress deprivation is sex-dependent

Enhancement of A Cationic Surfactant by Capping Nanoparticles: Synthesis, Characterization and Multiple Applications

Novel Gemini Cationic Surfactants: Thermodynamic, Antimicrobial Susceptibility, and Corrosion Inhibition Behavior against Acidithiobacillus ferrooxidans

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