A rapid and simple method for visualizing milky spots in large fixed tissue samples of the human greater omentum

Schurink, Bernadette; Cleypool, Cindy G. J.; Bleys, Ronald L. A. W.

doi:10.1080/10520295.2019.1583375

Cited by 13 publications

(19 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…OMSs could be identified in haematoxylin‐stained omental samples of all five cadavers, according to a previous description (Schurink et al. ). Of the five studied omenta, 18 OMSs (2–5 per omentum) and 7 omental arterial branches (1–2 per omentum) were sampled and processed for microscopic evaluation.…”

Section: Resultsmentioning

confidence: 99%

“…One or two large samples of approximately 6 9 4 cm were removed from each omentum. These samples were stained with haematoxylin (Schurink et al 2019) and evaluated for the presence of OMSs with the aid of a stereomicroscope (Leica EZ4, Nussloch, Germany), using both transmission and incident light. OMSs were identified, removed (with a small margin of surrounding tissue), and placed in PBS/sucrose at 4°C until further processing for microscopic investigation.…”

Section: Methodsmentioning

confidence: 99%

“…), are found in various animal species including humans (Michailova & Usunoff, ), and can be observed in large fixed omental tissue samples after staining with haematoxylin (Schurink et al. ) (Fig. ).…”

Section: Introductionmentioning

confidence: 99%

“…OMSs are cellular aggregates of macrophages, B-cells, and T-cells (Shimotsuma et al 1991;Liu et al 2015). They develop during ontogeny (Krist et al 1997), are found in various animal species including humans (Michailova & Usunoff, 2004), and can be observed in large fixed omental tissue samples after staining with haematoxylin (Schurink et al 2019) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sympathetic nerve tissue in milky spots of the human greater omentum

et al. 2019

Self Cite

View full text Add to dashboard Cite

Omental milky spots (OMSs), small lymphoid structures positioned in the greater omentum, are involved in peritoneal immune homeostasis and the formation of omental metastases. Sympathetic nerve activity is known to regulate immune function in other lymphoid organs (e.g. spleen and lymph nodes) and to create a favourable microenvironment for various tumour types. However, it is still unknown whether OMSs receive sympathetic innervation. Therefore, the aim of this study was to establish whether OMSs of the adult human greater omentum receive sympathetic innervation. A total of 18 OMSs were isolated from five omenta, which were removed from 3% formaldehyde‐perfused cadavers (with a median age of 84 years, ranging from 64 to 94). OMSs were embedded in paraffin, cut and stained with a general (PGP9.5) and sympathetic nerve marker (TH and DBH), and evaluated by bright field microscopy. A T‐cell, B‐cell, and macrophage staining was performed to confirm OMS identity. In 50% of the studied OMSs, sympathetic nerve fibres were observed at multiple levels of the same OMS. Nerve fibres were represented as dots or elongated structures and often observed in relation to small vessels and occasionally as individual structures residing between lymphoid cells. The current study shows that 50% of the investigated OMSs contain sympathetic nerve fibres. These findings may contribute to our understanding of neural regulation of peritoneal immune response and the involvement of OMSs in omental metastases.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sympathetic nerve tissue in milky spots of the human greater omentum

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Існують істотні розбіжності його точної морфології щодо наявності в його структурі лімфатичних вузликів, топографії «молочних плям». Дискусійним є питання чи відноситься великий чепець людини до периферійних органів імунної системи [1,2,3,4,5]. Тому із вивченням теоретичних аспектів структурної організації великого чепця як імунокомпентентного органа тісно пов'язано і вирішення практичної задачі -пошук і виділення із нього факторів, які регулюють імунні реакції в організмі [6].…”

Section: полтавський державний медичний університет українаunclassified

Histostructure of the Normal Human Greater Omentum and in Peritonitis

Stepanchuk¹,

Федорченко²,

Tarasenko³

et al. 2021

Ukr. ž. med. bìol. sportu

View full text Add to dashboard Cite

The purpose of the work was to study the histological structure of the greater omentum in the people of second mature and elderly age in normal condition and in peritonitis. Materials and methods. The materials of the study were 40 greater omenta of deceased people (20 males and 20 females) of the second mature and elderly age without pathology in the abdominal cavity and 10 greater omenta of deceased people from peritonitis taken in accordance with the cooperation agreement. Pieces of greater omenta were fixed with a 10% solution of neutral formalin. Paraffin blocks were made according to the traditional method, followed by making sections that were stained with hematoxylin and eosin. Histological specimens were studied using a light microscope "MICROmed" XS-3320 in transmitted light using an increase of ×40, ×100, ×400 with the photographing of micropreparations on the camera Ningero Sheng Heng Optics and Electronics Co. Results and discussion. The findings of the study showed that the histological structure of the tissue of the greater omentum in women and men of these ages has common structural features. Histological sections stained with hematoxylin and eosin revealed that normally the outside of the greater omentum is covered with a solid layer of mesotheliocytes, located on the basal plate, deeper there were “milk spots”, white adipocytes, which formed individual fat particles, separated by connective tissue fibers. “Milk spots” do not have capsules, in contrast to lymph nodes, and contain lymphocytes and macrophages that emerge from their fenestrated postcapillary venules and migrate into the abdominal cavity through “stigmas”. Normally, active and inactive “milk spots” are present in the greater omentum in adults. “Milk spots” had an oval, irregular and spherical shape and different sizes. In the thickness of the tissue of the greater omentum there were lymph nodes, which, in contrast to the "milk spots" on the outside were covered with a connective tissue capsule. In the thickness of the nodule, trabeculae extend from the capsule. Marginal and medullary sinuses were determined. Lymphocytes were the main cellular component of lymph nodes. Conclusion. In peritonitis, disruption of the continuous mesothelial cover of the greater omentum, resulted from disintegration of mesothelial cells; some areas of their adhesion and desquamation were observed. Areas with inflammatory infiltration, plethora of arterial and venous microvessels and an increase in active “milk spots” with a high content of lymphocytes were found in the fat particles of the greater omentum

show abstract

Endogenous ozone as a regular reactive oxygen species in (patho) physiology

Onyango

2023

Advances in Redox Research

View full text Add to dashboard Cite

A rapid and simple method for visualizing milky spots in large fixed tissue samples of the human greater omentum

Cited by 13 publications

References 12 publications

Sympathetic nerve tissue in milky spots of the human greater omentum

Sympathetic nerve tissue in milky spots of the human greater omentum

Histostructure of the Normal Human Greater Omentum and in Peritonitis

Endogenous ozone as a regular reactive oxygen species in (patho) physiology

Contact Info

Product

Resources

About