A rapid and robust liquid chromatography/tandem mass spectrometry method for simultaneous analysis of anti-tuberculosis drugs—Ethambutol and pyrazinamide in human plasma

Gong, Zhaoning; Basir, Yousef J.; Chu, David C.; McCort-Tipton, Melanie

doi:10.1016/j.jchromb.2009.04.023

Cited by 38 publications

(21 citation statements)

References 6 publications

(6 reference statements)

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“…Though methods are available for Liquid Chromatography Mass Spectrometer tandem Mass Spectrometry (LC-MS/MS) [14][15][16] , it is found that the chromatographic conditions and extraction processes were highly expensive as they use cartridges, and high solvent usages 17 . The assay method of Ethambutol hydrochloride in the tablets given in the Chinese Pharmacopoeia (CHP) involves repeated chloroform extraction from a basic aqueous solution and subsequent nonaqueous titration 18 .…”

Section: Fig 1: Chemical Structure Of Ethambutolmentioning

confidence: 99%

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2016

IJPSR

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Simple and sensitive extractive spectrophotometric methods for the determination of Ethambutol in pure form and in pharmaceutical formulations using triphenyl methane dyes have been developed. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with triphenyl methane dyes viz., bromocresol green (BCG), bromocresol purple (BCP) and bromophenol blue (BPB) in acidic medium. The extracted complexes formed with BCG, BCP and BPB showed absorbance maxima at 420, 419 and 415 nm respectively. The stoichiometry of the ion-pair complex is found to be 1:1 in each case. Beer's law is obeyed in the concentration ranges 2.0-25, 3.0-30 and 4.0-40 μg/ml with BCG, BCP and BPB respectively. The effect of concentration of dye, pH, and interference of excipients have been studied for optimization. The limits of detection and quantification have been determined for all the three methods. These methods have been validated as per the guidelines of ICH. The results of analysis were validated statistically through recovery studies.

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Section: Fig 1: Chemical Structure Of Ethambutolmentioning

confidence: 99%

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2016

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“…All can be ionised in positive mode, and C18 is the most popular analytical column used. Many of the methods published can quantify multiple drugs simultaneously [160][161][162], which is advantageous when patients are being treated with several drugs.…”

Section: Antibioticsmentioning

confidence: 99%

Therapeutic drug monitoring and LC–MS/MS

Adaway

Keevil

2012

Journal of Chromatography B

128

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“…Its simplified formula is C 10 H 24 N 2 O 2 ·2HCl (277.23 g mol À1 ) [1,2]. In the past fifty years, many procedures have been presented for the quantification of ethambutol in various matrices (such as urine, human plasma and pharmaceutical formulations), from simple analytical procedures, such as titrimetry [3], to more modern and refined techniques, such as mass-spectrometry [4,5]. Most of these techniques focus on analytical separations under chromatographic (including thin layer chromatography) [4][5][6][7][8] or electrophoretic conditions [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…In the past fifty years, many procedures have been presented for the quantification of ethambutol in various matrices (such as urine, human plasma and pharmaceutical formulations), from simple analytical procedures, such as titrimetry [3], to more modern and refined techniques, such as mass-spectrometry [4,5]. Most of these techniques focus on analytical separations under chromatographic (including thin layer chromatography) [4][5][6][7][8] or electrophoretic conditions [9][10][11][12]. In some cases, ethambutol undergoes to chemical reactions such as complexation with copper ions [10], formation of ionic-pairs [13,14] and derivatisation processes with different reagents [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Flow Injection Analysis of Ethambutol in Antituberculosis Drugs Using a Graphite‐Paraffin Electrode as Amperometric Detector

et al. 2011

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A graphite-paraffin composite electrode has been used for the determination of ethambutol, an antituberculosis drug, in pharmaceutical samples by anodic oxidation. Voltammetric studies were used to characterise the electrochemical behaviour of the ethambutol in different pH solutions (3.0, 8.0 and 12.0). Quantification was carried out using amperometric detection. The detector was assembled in a flow injection apparatus and operated at + 1.1 V (vs. Ag/AgCl (NaCl,sat) ). The conditions for the best electrodes response were determined by studying the influence of sample volume and flow rate. The linear response for the method was extended up to a 1.5 mmol L À1 ethambutol solution with a detection limit of 0.1 mmol L À1. The repeatability for successive injections of 1.0 mmol L À1 ethambutol solution was 0.9 % (n = 26), demonstrating a good performance of the developed amperometric sensor. The analytical frequency was calculated as approximately 47 determinations h À1. Two different commercial samples were successfully analysed and the results were in good agreement with those obtained by using spectrophotometry. The advantages of the proposed amperometric method over the reference method include simplicity, low consumption of reagents and increased analytical frequency.

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A rapid and robust liquid chromatography/tandem mass spectrometry method for simultaneous analysis of anti-tuberculosis drugs—Ethambutol and pyrazinamide in human plasma

Cited by 38 publications

References 6 publications

Untitled

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Therapeutic drug monitoring and LC–MS/MS

Flow Injection Analysis of Ethambutol in Antituberculosis Drugs Using a Graphite‐Paraffin Electrode as Amperometric Detector

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