A Rapid and Practical Method of Determining Extractable White Sugar as may be Applied to the Evaluation of Agronomic Practices and Grower Deliveries in the Sugar beet Industry

Dexter, S. T.; Frakes, M. G.; Snyder, F. W.

doi:10.5274/jsbr.14.5.433

Cited by 78 publications

(50 citation statements)

References 2 publications

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“…Sugar extraction=V4-SM-0.6 (Dexter et al, 1967). Extratability %= Sugar extraction/ Pol % where: V 1 -Sodium; V 3 -Alpha amino-N; V 2 -Potassium; V4-Pol% Sugar yield ton/fed.…”

Section: Methodsmentioning

confidence: 99%

Sugar beet performance under newly reclaimed soils conditions of Sinai, Egypt

Nasr

El-Razek

2008

Sugar Tech

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The present investigation was carried out to study the effect of planting and harvesting time on yield and quality of sugar beet cultivars under North Sinai conditions.Two field experiments were conducted in a newly reclaimed sandy soil in a private farm at North Sinai region to study the effect of three sowing dates (1 st Aug., 1 st Sep. and 1 st Oct.) and three harvesting dates (170, 190 and 210 days after planting) on yield and quality of six sugar beet cultivars, viz.,Pamela, Hipoly2, Pleno, Monte Bianco, Oscar poly and Gloria. The results obtained from this study showed that sowing dates had significant effects on sucrose and purity per cent, as well as, root and sugar yields/fed in both seasons. The highest root and sugar yield were obtained from the 1 st Sept. sowing. Harvesting after 210 days from sowing recorded the highest root weight, sucrose and purity per cent as well as root and sugar yields/fed. Oscar poly variety recorded the highest root yield but, Monte Bianco cultivar surpassed all cultivars in sugar yield. The interaction between each two factors under study was insignificant. The response equation of root yield/ fed to delaying harvest showed diminishing returns. The results revealed that a higher predicted root yield than that retained herein, could have been obtained if harvest was delayed beyond 210 days after sowing.

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“…Sugar extraction=V4-SM-0.6 (Dexter et al, 1967). Extratability %= Sugar extraction/ Pol % where: V 1 -Sodium; V 3 -Alpha amino-N; V 2 -Potassium; V4-Pol% Sugar yield ton/fed.…”

Section: Methodsmentioning

confidence: 99%

Sugar beet performance under newly reclaimed soils conditions of Sinai, Egypt

Nasr

El-Razek

2008

Sugar Tech

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“…Sucrose concentration and purity were used to calculate extractable sucrose concentration (Dexter et al 1967). Extractable sucrose is that portion of the total sucrose in the root that can be extracted using standard sugar factory operations, expressed as kg sucrose per Mg of roots.…”

Section: Methodsmentioning

confidence: 99%

“…Sucrose was determined polarimetrically (McGinnis 1982). Purity (sucrose as a percent of dry substance) was determined using the procedures described by Dexter et al (1967). Twenty-gram brei (fine beet particles -the product of a beet saw) samples were oven dried at 80°C to determine dry matter concentration.…”

Section: Methodsmentioning

confidence: 99%

Characterizing sugarbeet varieties for postharvest storage losses is complicated by environmental effects and genotype × environment interactions

Campbell¹,

Klotz²

2007

Can. J. Plant Sci.

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. 2007. Characterizing sugarbeet varieties for postharvest storage losses is complicated by environmental effects and genotype × × environment interactions. Can. J. Plant Sci. 87: 121-127. Each year millions of tons of sugarbeet (Beta vulgaris L.) roots are stored in large exposed piles prior to processing. During postharvest storage, respiration and invert sugar formation consume sucrose and even a small reduction in these losses would have substantial economic impact. This study investigated the relative importance of hybrid, environment, and hybrid × environment interactions and examined their implications in characterizing hybrids for sucrose loss during storage or developing hybrids with improved storage properties. Glucose, fructose, and extractable sucrose concentrations and respiration rate were measured 30 and 120 d after harvest (DAH) on five hybrids produced in six environments. Environment effects were significant on both dates for all traits except fructose 30 DAH. Significant hybrid × environment interactions were observed for respiration rate 30 and 120 DAH, for extractable sucrose 120 DAH, and for glucose concentration 30 DAH. The only trait with a significant hybrid main effect was extractable sucrose 30 DAH. For the 90 d between measurements, extractable sucrose losses for individual hybrid-environment combinations ranged from 1 to 63% of the sucrose available 30 DAH. It appeared that large environmental impacts and hybrid × environment interactions, compared to the relatively small hybrid influences, would complicate selecting parental lines with all or most of the storage traits desired. Furthermore, a comprehensive evaluation of commercial hybrids or breeding lines for storage traits would require considerable resources. Efforts to understand the impact of production practices and growing season environment on storage properties would probably be more productive than attempting to produce commercial hybrids with improved storage characteristics.

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“…The reducing sugar content of the stored roots was determined on a clarified extract prepared by the method of Dexter et al (4).…”

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Enzymes Involved in the Postharvest Degradation of Sucrose in Beta vulgaris L. Root Tissue

Wyse¹

1974

Plant Physiol.

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The reducing sugar content of sugar beet (Beta vulgaris L.) roots increased during 30 days of storage at 21 C and 160 days at 5 C as a result of an increase in acid invertase activity. Sucrose synthetase and neutral invertase activities were high at harvest but declined during storage, thus showing no relationship with postharvest reducing sugar accumulation in sugar beet roots. Acid a-glucosidase activity was detected in fresh roots but showed no activity with sucrose as a substrate.The sucrose content of sugar beet roots declines after harvest as a result of sucrose conversion to reducing sugars and oligosaccharides (20). At storage temperatures above 5 C reducing sugars (glucose and fructose) accumulate, while at temperatures below 5 C both raffinose and reducing sugars accumulate (20). The objective of this study was to determine which enzymes are involved in sucrose degradation in sugar beet roots during storage.Acid invertase activity is normally very low in fresh sugar beet roots, but induction of invertase activity by aging beet disks in water has been well documented (1,15,19 operations were carried out at 0 to 5 C. Roots were peeled, diced, and homogenized in a VirTis homogenizer' with an equal w/v ratio of grinding medium (0.05 M potassium phosphate, pH 7.2; 0.1 mm EDTA; 1 mM 2-mercaptoethanol; 10 mM Na2SO.). The homogenate was strained through two layers of dacron ninon and centrifuged at 16,000g for 20 min. The supernatant liquid was dialyzed overnight against 10 volumes of either grinding medium for neutral invertase and sucrose synthetase assays, or acetate buffer (0.05 M acetate, pH 5.0; 0.1 mM EDTA; 1 mm 2-mercaptoethanol; 10 mm Na,SO8) for acid invertase assay. The extract dialyzed at pH 5 was centrifuged at 1 6,000g for 10 min before assay and the pellet discarded.Assay. The standard assay mixture for neutral invertase contained 0.16 ml of buffer (8 ltmoles of potassium phosphate, pH 7.2; 0.016 lumole EDTA; 80 jumoles of sucrose) and 0.04 ml of the enzyme preparation. Normal assay time was 30 min at 35 C. Assay mixtures for sucrose synthetase were the same as for neutral invertase except that 10 ,u1 of 0.05 M UDP were added and only 0.03 ml of the enzyme preparation was used. Assay mixtures for acid invertase were the same as for neutral invertase except that the phosphate buffer was replaced by acetate at pH 5.0. The reactions were stopped by adding 1 ml of Somogyi's (18) copper reagent and reducing sugars determined by the method of Nelson (14). Glucose liberation, in assays in which maltose was the substrate, was determined by the glucose oxidase/peroxidase method with a commercial reagent (Glucostat, Worthington2).Protein was determined by the method of Lowry (9). The reducing sugar content of the stored roots was determined on a clarified extract prepared by the method of Dexter et al. (4). RESULTS AND DISCUSSIONIn a preliminary experiment a survey of enzymes which may degrade sucrose in fresh sugar beet roots was made. Sucrose, raffinose, and maltose were used as substrates and a...

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A Rapid and Practical Method of Determining Extractable White Sugar as may be Applied to the Evaluation of Agronomic Practices and Grower Deliveries in the Sugar beet Industry

Cited by 78 publications

References 2 publications

Sugar beet performance under newly reclaimed soils conditions of Sinai, Egypt

Sugar beet performance under newly reclaimed soils conditions of Sinai, Egypt

Characterizing sugarbeet varieties for postharvest storage losses is complicated by environmental effects and genotype × environment interactions

Enzymes Involved in the Postharvest Degradation of Sucrose in Beta vulgaris L. Root Tissue

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