A rapid and high‐throughput method for the determination of serum uric acid based on microarray technology and nanomaterial

Abstract

Serum uric acid (SUA) is a new therapeutic target for non-alcoholic fatty liver disease (NAFLD). In this study, we introduced a chemiluminescence (CL) method combined with microarray technology and a simple fabrication procedure to obtain a highly sensitive SUA probe based on a mesoporous metal oxide nanomaterial. The high-throughput method was based on the generation of H O from SUA by immobilized uricase and its measurement by a CL reaction catalyzed by mesoporous metal oxide nanomaterials. The CL probe was designed for SUA The linear range of the uric acid concentration was 0.6-9 μM and the detection limit was 0.1 μM. In comparison with the other SUA detection techniques, this method has the advantages of a low detection limit, high sensitivity and simplicity. A new sensitive high-throughput approach was obtained for the determination of SUA.

A facile fluorescence turn‐on biosensor customized for monitoring of protein kinase activity based on carboxylic carbon nanoparticle–peptide complexes

“…In recent years, to develop a novel technique for life sciences and biotechnology, significant numbers of nanomaterials with unique chemical and physical properties were combined with specific recognition elements. [ 27–29 ] Gold nanoparticles, carbon nanomaterials, and silica nanoparticles have emerged. Additionally, they have widespread applications, particularly in drug delivery, therapy, and diagnostics in vivo and in vitro .…”