A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria

Hettiarachchi, Sachithra Amarin; Lee, Jehee; Kwon, Young-Kyung; Zoysa, Mahanama De; Moon, Song Hee; Jo, Eunyoung; Kim, Eun Young; Kang, Do‐Hyung; Heo, Soo‐Jin; Oh, Chulhong

doi:10.4014/jmb.1703.03012

Cited by 11 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pure colonies of indicator and test bacteria were prepared by streaking a loopful of stock onto agar plates. For the detection of antimicrobial activity, the colony picking method ( 62 ) was applied initially and R2A agar and PCA agar were used. Subsequently, the antimicrobial activity testing procedure was modified by application of an agar spot test ( 63 ).…”

Section: Methodsmentioning

confidence: 99%

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Sedláček

Holochová

Sobotka³

et al. 2021

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Sedláček

Holochová

Sobotka³

et al. 2021

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…In the antimicrobial test of cell growth, the secretion process is controlled by the cell to cell interaction (Goers et al 2014). However, in the CFS, the pure colony of isolates may have already secreted extracellular antimicrobial compound (Hettiarachchi et al 2017). CFS produced by BAL contains organic acids, hydrogen peroxide, and diacetyl (Hor and Liong 2014).…”

Section: Detection Of Antimicrobial Activitymentioning

confidence: 99%

The Isolation and molecular identification of antimicrobial-producing Lactic Acid Bacteria from chao, South Sulawesi (Indonesia) fermented fish product

2019

View full text Add to dashboard Cite

Abstract. Nurhikmayani R, Daryono BS, Retnaningrum E. 2019. Isolation and molecular identification of antimicrobial-producing Lactic Acid Bacteria from chao, South Sulawesi (Indonesia) fermented fish product. Biodiversitas 20: 1063-1068. Chao is traditional fermented fish made of anchovy (Stolephorus sp.), rice, salt, and fermented naturally on controlled environment. The product comes from Pangkep Regency, South Sulawesi. Chao has the potential for the source of high-grade lactic acid bacteria isolates, especially for antimicrobial compound. Antimicrobial compound produced by lactic acid bacteria is a biopreservative compound with many advantages. The purposes of this investigation were to isolate antimicrobial-producing lactic acid bacteria from chao and to identify their isolates based on the molecular properties. Lactic acid bacteria were isolated from chao using Man Ragosa Sharpe Agar (MRSA) medium added with 0.1 % CaCO3, followed by the ability test to produce antimicrobial compound against Staphylococcus aureus FNCC 0047 as an indicator. The spectrum of crude antimicrobial as cell-free supernatant (CFS) was determined by antagonism against S. aureus FNCC 0047 and Escherichia coli FNCC 0049 on agar well diffusion test. Selected isolates of antimicrobial-producing lactic acid bacteria molecularly identified from 16S rRNA marker using primer 27F and 1492R. We obtained four isolates of lactic acid bacteria that showed the antimicrobial activity. This crude antimicrobial compound could inhibit both gram-positive Staphylococcus aureus FNCC0047 and gram-negative bacteria Escherichia coli FNCC0049. The highest crude antimicrobial activity of isolates was observed on IB1C strain. The results of molecular characterization suggested that the IB1C and IB3B strain were identified as Lactobacillus plantarum, whereas IB3E and IB3F were identified as Pediococcus pentosaceus.

show abstract

“…The primary screening for antimicrobial activities was performed by the colony picking method according to Hettiarachchi et al [20] with slight modification (Figure 2). In brief, the test strains were prepared into 4 mL of marine broth (5.0 g/L peptone from soya bean, 1.0 g/L yeast extract, 0.3 g/L dipotassium hydrogen phosphate, 1.0 g/L glucose, 5.0 g/L sodium chloride), shaking at 120 rpm, and incubation at room temperature (approximately 30 • C due to the in-situ temperature of 28.1-28.3 • C when collecting samples) for 18-24 h until the concentration of bacteria was a measure of the 0.5 McFarland standard [24], approximately 10 8 cfu/mL.…”

Section: Primary Screening Of Antimicrobial Activities By Colony Picking Methodsmentioning

confidence: 99%

“…The sequences were alignment with SeqMan ™ II (DNAStar) and compared with the highest 16s rRNA gene homologs on the gene bank (National Center for Biotechnology Information NCBI) using Nucleotide blast/NCBI (http://blast.ncbi.nlm.nih.gov/Blast. cgi) [20].…”

Section: Isolation Of Sponge-associated Bacteria-sab and Test Strain Vibrio Spmentioning

confidence: 99%

Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam

Mien

Ben

et al. 2020

JMSE

View full text Add to dashboard Cite

Bacteria associated with invertebrates are considered as good sources for biologically active compounds. Sponge-derived bacteria were screened for antimicrobial activities, the presence of the cluster genes of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), and through a colony picking method. Crude extracts of broth cultures were tested for microbial inhibition. Eleven out of 25 isolated strains showed inhibition of at least one of eight tested indicator microorganisms. Antimicrobial activities were observed in the strains coded HM5, HM6, and HM9 with the presence of NRPS and PKSII genes, whereas the isolate HM21 held both NRPS and PKSII and inhibited only the growth of Bacillus subtilis by the well diffusion method and only inhibited Serratia marcescens by the colony picking method. Two isolates, HM5 and HM6, belonged to the species of Bacillus. Interestingly, the isolate HM9 was nearest to Streptomyces mexicanusT NBRC100915 (GenBank accession number AB249966) with 94% sequence similarity. This potent strain HM9 could possibly be considered as a new species and a good source for bioactive compound discovery. Some isolates showed NRPS/PKS genes but did not exhibit antimicrobial activity. Thus, we suggested that both molecular and traditional methods should be conducted for the screening of antimicrobial producers.

show abstract

A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria

Cited by 11 publications

References 0 publications

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

The Isolation and molecular identification of antimicrobial-producing Lactic Acid Bacteria from chao, South Sulawesi (Indonesia) fermented fish product

Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam

Contact Info

Product

Resources

About