Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam

Mien, Pham Thi; Ha, Dao Viet; Ben, Hoang Xuan; Chen, Bin; Liu, Lan; Thu, Phan Minh

doi:10.3390/jmse8080594

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Meanwhile, a recent whole-genome mining study estimated that a lower percentage (~31%) of Firmicutes carried NRPS and PKS gene clusters [ 30 ]. In a recent study, Mien et al (2020) also reported a lower percentage for NRPS and PKS-I genes (24.0% and 0%, respectively), but higher for PKS-II genes (20.0%), after PCR amplification of 25 marine bacteria isolated from the Central Sea region of Vietnam [ 31 ]. Similarly, a lower percentage of NRPS gene (38%) and PKS-I gene (38%) but a higher percentage of PKS-II (54%) were observed among 50 invertebrate-associated bacteria from the Red Sea [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea

et al. 2021

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Vibriosis in farmed animals is a serious threat to aquaculture worldwide. Using probiotics and anti-Vibrio antimicrobial substances in aquaculture systems can be a means of preventing Vibrio infections. Therefore, we aimed to characterize and compare 16 potential anti-Vibrio probiotics (Vi+) isolated from marine sponges and fish intestines collected from the Vietnam Sea, as well as an anti-Vibrio bacteriocin to fully explore their application potentials. 16S rRNA sequencing confirmed all Vi+ to be Bacillus species with different strain variants across two sample types. An obvious antimicrobial spectrum toward Gram-negative bacteria was observed from intestinal Vi+ compared to sponge-associated Vi+. The reason was the higher gene frequency of two antimicrobial compounds, non-ribosomal peptides (NRPS) and polyketide type-I (PKS-I) from intestinal Vi+ (66.7%) than sponge-associated Vi+ (14.3% and 0%, respectively). Additionally, a three-step procedure was performed to purify an anti-Vibrio bacteriocin produced by B. methylotrophicus NTBD1, including (i) solvent extraction of bacteriocin from cells, (ii) hydrophobic interaction chromatography, and (iii) reverse-phase HPLC. The bacteriocin had a molecular weight of ~2–5 kDa, was sensitive to proteolysis and thermally stable, and showed a broad antimicrobial spectrum, all of which are essential properties for promising feed additives. This study provides necessary information of the potential of probiotic Bacillus species with anti-Vibrio antimicrobial properties to study their further use in sustainable aquaculture.

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Section: Discussionmentioning

confidence: 99%

Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea

et al. 2021

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“…Vietnam. These sponges are recognized by their dark brown exterior and yellow interior (Mien et al, 2020;Pham et al, 2013) (Figureure 1).…”

Section: Identification Of Marine Sponge Ra3 and Rb1mentioning

confidence: 99%

Identification of The Secondary Metabolite Derived from Aaptos spp. and its Antibacterial Activity

Utami,

Paramita,

Agustina

et al. 2024

Indonesian J Pharm

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Many novel bioactive compounds have been found to be most abundantly produced by sponges. Over the years, Indonesian sponges have played an important role in expanding our understanding of chemical diversity and biological activity. Among those sponges, the sponge genus Aaptos has been shown to have antimicrobial activity. Antimicrobial resistance and the spread of infections in the immunocompromised population have all contributed to an increase in the appearance and severity of these infections in recent decades. Finding an alternative antimicrobial drug from marine secondary metabolites will be beneficial. The current study looks into the profile of bioactive compounds derived from the sponge Aaptos. The sponge was collected from Raja Ampat Island, Indonesia. This sample was extracted with ethanol and an ethyl acetate-methanol solvent. Furthermore, Thin Layer Chromatography (TLC), High-performance liquid chromatography with diode-array detection (HPLC-DAD) and Principal Component Analysis (PCA) were used to map the secondary metabolite related to antimicrobial activity in both extracts. TLC profiling revealed potential alkaloid and terpenoid compounds from both ethanol extract and ethyl acetate-methanol. The HPLC-DAD and PCA results revealed that one of the ethanol crude extracts had a similar profile to Ciprofloxacin, Chloramphenicol, and Ampicillin. These antibacterial profiles open up new research avenues, with the ultimate goal of contributing to the development of new and effective antimicrobial drugs. This research also helps with the bioprospecting of marine sponges from Indonesian sea water.

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Exploring Newer Biosynthetic Gene Clusters in Marine Microbial Prospecting

2022

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Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam

Cited by 3 publications

References 32 publications

Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea

Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea

Identification of The Secondary Metabolite Derived from Aaptos spp. and its Antibacterial Activity

Exploring Newer Biosynthetic Gene Clusters in Marine Microbial Prospecting

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