A rapid [3H]glucose incorporation assay for determination of lymphoid cell-mediated inhibition of Candida albicans growth

Djeu, Julie Y.; Parapanissios, A.; Halkias, Demetrios G.; Friedman, Herman

doi:10.1016/0022-1759(86)90505-3

Cited by 39 publications

(20 citation statements)

References 9 publications

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“…In RPMI 1640 medium supplemented with FCS, C. albicans grew in hyphal forms and the mycelial colonies firmly attached to the substratum of the microplate as described previously (2). To measure the hyphal growth of Candida conventionally and quantitatively, development of a photometric method was attempted.…”

Section: Resultsmentioning

confidence: 99%

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A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

Abe

Satoh

Tokuda

et al. 1994

Microbiology and Immunology

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A staining method with crystal violet (CV) was demonstrated to be useful for a simple, quick and objective assessment of in vitro growth inhibitory activity of leukocytes against Candida albicans cells. Candida cells incubated with murine neutrophils or macrophages for 14 hr in microwells were stained with CV and, after washing with 0.25% sodium dodecyl sulfate (SDS), treated with isopropanol containing HC1 (0.04 N) to extract Candida cell-bound CV. Then the absorbance at 590 nm of the isopropanol extract was photometrically measured. The results showed that the photometrical absorbance was proportional to the amount of 3H-glucose taken up by C. albicans cells, which reflected the number of viable Candida cells.

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Section: Resultsmentioning

confidence: 99%

“…Djeu et al developed a 3 H-glucose incorporation assay for determination of leukocyte-mediated inhibition of Candida growth (2). This assay in microwell plates made possible accurate and objective measurement of Candida growth under the influence of a limited number of effector cells.…”

mentioning

confidence: 99%

A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

Abe

Satoh

Tokuda

et al. 1994

Microbiology and Immunology

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“…Examination of even more subtle morphologic effects is possible and would be applicable to a wider range of fungi (121,136), but such approaches are highly subjective. Measurements of uptake of radiolabeled metabolites (30,31,35,56) or reduction of colored substrates (79,124) have been used to assess phagocyte-mediated damage to C. albicans, Aspergillus flumigatus, Rhizopus arrhizus (=R. oryzae), and Coccidioides immitis and could readily be adapted to measuring the effect of antifungal drugs on fungi (88,140,143).…”

Section: Studiesmentioning

confidence: 99%

Antifungal Susceptibility Testing

Yuxin¹

2016

Clinical Microbiology Procedures Handbook, Fourth Edition

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“…3 H]glucose incorporation assay technique (6). One hundred microliters of a neutrophil suspension or macrophage suspension (1 ϫ 10 6 cells/ml) in complete medium was seeded into triplicate wells of flat-bottom microplates (100 l/well), and then 100 l of a C. albicans suspension (4 ϫ 10 4 cells/ml) was added to these wells, yielding an effector cell:target cell (E/T) ratio of 25:1.…”

Section: Animalsmentioning

confidence: 99%

Therapeutic Efficacy of Human Macrophage Colony-Stimulating Factor, Used Alone and in Combination with Antifungal Agents, in Mice with SystemicCandida albicansInfection

Kuhara¹,

Uchida

Yamaguchi

2000

Antimicrob Agents Chemother

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We examined the in vivo activity of human macrophage colony-stimulating factor (hM-CSF) against lethal Candida albicans infection in mice. In C. albicans-infected mice which had been immunosuppressed with cyclophosphamide, treatment with hM-CSF at a daily dose of 8 ؋ 10 5 units/kg of body weight or greater slightly but significantly prolonged survival. Furthermore, the therapeutic efficacy of amphotericin B (AMPH-B) in infected mice was enhanced by its combined use with hM-CSF, while that of fluconazole (FLCZ) was not. The activities of peritoneal macrophages and neutrophils from mice administered hM-CSF plus AMPH-B in combination for inhibition of hyphal growth of C. albicans cells and intracellular phagocytosis and killing of the cells were greater than those of comparable phagocytic cells from control mice to which hM-CSF plus AMPH-B was not administered. These results suggest that intravenous administration of hM-CSF augments the efficacy of AMPH-B by enhancing the antifungal activities of macrophages and neutrophils. Therefore, it is expected that therapy with the combination AMPH-B and hM-CSF could improve the efficacy of AMPH-B and reduce the therapeutic dose of the antifungal drug that is required.

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A rapid [3H]glucose incorporation assay for determination of lymphoid cell-mediated inhibition of Candida albicans growth

Cited by 39 publications

References 9 publications

A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

Antifungal Susceptibility Testing

Therapeutic Efficacy of Human Macrophage Colony-Stimulating Factor, Used Alone and in Combination with Antifungal Agents, in Mice with SystemicCandida albicansInfection

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