A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone

Vaitukaitis, Judith L.; Braunstein, Glenn D.; Ross, Griff T.

doi:10.1016/0002-9378(72)90553-4

Cited by 853 publications

(143 citation statements)

References 12 publications

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…Metabolism and Diseases of the Digestive System (Vaitukaitis et al, 1972) in a radioimmunoassay modified from the method of Orth (1974), in which polyethylene glycol was used to precipitate the bound hormone. The influence of cell proliferation on 3-hCG secretion was examined in 4 cell lines by trypsinizing confluent cultures, replating all the cells in similar flasks, where they underwent a brief wave of proliferation, and assaying the medium, which was renewed every day for 7 days for ,B-hCG content.…”

Section: Methodsmentioning

confidence: 99%

Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

Easty¹,

Easty²,

Carter³

et al. 1981

Br J Cancer

170

View full text Add to dashboard Cite

Summary.-Ten cell lines of human squamous carcinomas of the tongue and larynx have been established from surgical specimens removed from 36 unselected patients, in order to provide systems for investigating the invasive and tissue-destructive capacity of squamous carcinomas of the head and neck. The morphology, ultrastructure and growth characteristics of the 10 lines are described. Detailed cytogenetic analysis of the first 4 lines indicates that each is karyotypically unique, with no evidence of cross-contamination. Nine of the 10 cell lines secrete immunoreactive / human chorionic gonadotrophin (/-hCG) in the culture medium. No correlation was demonstrated between the ability of the cell lines to secrete plasminogen activator and their capacity to grow in soft agar or as xenografts in immune-deficient mice.

show abstract

Section: Methodsmentioning

confidence: 99%

Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

Easty¹,

Easty²,

Carter³

et al. 1981

Br J Cancer

170

View full text Add to dashboard Cite

show abstract

“…This latter method has been found to produce mice more receptive to xenografted tumours (Steel et al, 1978) and these mice were used for later pas- (Nakane & Kawais, 1974;Heyderman, 1979;Heyderman & Neville, 1976). The first antibody was directed against the beta subunit of HCG, to avoid cross-reactivity with the other glycoprotein hormones (Vaitukaitis et al, 1972). The second antibody (sheep anti-rabbit) was raised and purified at this Institute.…”

Section: Case Reportmentioning

confidence: 99%

A human testicular teratoma serially transplanted in immune-deprived mice

Selby¹,

Heyderman²,

Gibbs³

et al. 1979

Br J Cancer

View full text Add to dashboard Cite

Summary.-Serial transplantation of an HCG-producing human testicular teratoma in immune-deprived mice is described. The xenografted tumour was compared to the tumour of origin in histology, immunohistochemistry (using an immune peroxidase technique to localize HCG) autoradiography, marker production and growth rate. It is concluded that the xenograft retained the characteristics of the original tumour with the exception of a reduction in HCG-producing elements at transplantation beyond 5 serial passages.

show abstract

“…Interestingly, this region appears to be equally reactive in the native hormone, in the free subunit, and in the reduced and alkylated , subunit, suggesting that it is a structure that is loosely arranged at the surface of the hornone molecule and that the antibody combining sites recognize elements of primary structure. Chen, Matsuura, and colleagues have studied this phenomenon extensively and suggest that most of the antibodies bind to the last 15 COOH-terminal residues (35,36 (20). Other monoclonal antibodies listed in the figure as recognizing some elements of this general region have been defined by virtue of their ability to compete with each other for binding (32).…”

Section: Immunochemical Considerationsmentioning

confidence: 99%

“…While the amino acid sequences of the a subunits of hLH and hCG were shown to be identical, there were minor differences in the structures of the 13 subunits as illustrated in the next section (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Vaitukaitis, Braunstein, and Ross found that occasionally, a rabbit, when immunized with the purified 1 subunit of hCG, made antibodies that were predominantly directed against this hormone and had limited cross-reactivity with hLH (20). This led to the development of radioimmunoassays that could routinely detect 1 ng/mL of hCG in serum in the presence of circulating hLH.…”

Section: Introductionmentioning

confidence: 99%

Development of an assay for a biomarker of pregnancy and early fetal loss.

Canfield

O’Connor

Birken

et al. 1987

Environ Health Perspect

View full text Add to dashboard Cite

Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; a and 1, with molecular weights of 14 kD and 23 kD, respectively. Its a subunit is identical in primary structure to its glycoprotein homologs, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH.Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. Antibody cooperative effects, in conjunction with kinetic advantages derived from the concentration factors by use of the sandwich assay technique (immunoradiometric assay, IRMA), have enabled development of extremely sensitive and specific measurement protocols for urinary hCG. The assay described herein permits the detection of pregnancy on an average 25.4 days after the first day of the preceding menses, as opposed to 29.5 days for conventional radioimmunoassay techniques. In addition, the greater sensitivity and specificity of this assay method has permitted the detection of episodes of fetal loss not detected by radioimmunoassay of urine specimens. A large scale epidemiological study is in progress using this assay technique as a way to identify pregnancies that are lost before becoming clinically apparent. This methodology provides a valuable tool for the determination of the rate of early fetal loss.

show abstract

A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone

Cited by 853 publications

References 12 publications

Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

A human testicular teratoma serially transplanted in immune-deprived mice

Development of an assay for a biomarker of pregnancy and early fetal loss.

Contact Info

Product

Resources

About