A rabbit bone tumor model for high-intensity focused ultrasound therapy

Abstract

The incidence of malignant bone tumor increases every year. Because the application of high-intensity focused ultrasound (HIFU) for the treatment of bone tumors is still at a nascent stage, it is essential to study the effectiveness of this technique in ideal animal models in order to obtain a beneficial reference for imaging studies. In this study, we established a VX2 malignant bone tumor model and evaluated this model by contrast-enhanced sonography and magnetic resonance imaging (MRI). The results show that all tumors were enhanced after injection of SonoVue. A contrast-enhanced MRI scan revealed obvious enhancement within the tumors. Histological examination revealed the presence of a large number of tumor cells. The model can serve as an ideal experimental model for the study of HIFU therapy in the treatment of malignant bone tumors and as a reference for imaging studies during follow-ups.

PMMA-Fe3O4 for internal mechanical support and magnetic thermal ablation of bone tumors

“…Standard methods to establish a rabbit tibial plateau bone tumor with VX2 mass/cells involve cutting the skin to expose the bone surface, using a burr to drill a hole in the tibial plateau, placing a tumor mass in the hole, sealing the hole via bone wax and suturing the skin incision 57, 58. These traditional procedures are complex, which may lower the efficiency of establishing the model.…”

“…These traditional procedures are complex, which may lower the efficiency of establishing the model. More importantly, in addition to the loss, the extensive trauma caused by drilling bone tissue will easily connect the drilled hole to the bone marrow cavity, which may change the model position and result in tumor metastasis, easily increasing the mortality 58, 59. Therefore, it is critical to modify the methods to develop a novel model.…”

Contrast-enhanced ultrasound evaluation of pancreatic cancer xenografts in nude mice after irradiation with sub-threshold focused ultrasound for tumor ablation

“…For blood-rich lesions, color Doppler flow imaging can be used to determine tissue survival by the reduction or elimination of the blood-flow distribution in the ablation lesions after HIFU irradiation [20, 21], which is more difficult to determine for lesions with less blood flow. In this study, because of the color Doppler flow imaging resolution and the limitation of lower blood flow velocity within small tumors, color Doppler flow imaging could only display the blood-flow signals of large, nourished blood vessels in the tumor periphery.…”