A Rab11- and Microtubule-Dependent Mechanism for Cytoplasmic Transport of Influenza A Virus Viral RNA

Amorim, Maria João; Bruce, Emily A.; Read, Eliot; Foeglein, Ágnes; Mahen, Robert; Stuart, Amanda D.; Digard, Paul

doi:10.1128/jvi.02606-10

Cited by 198 publications

(375 citation statements)

References 63 publications

(89 reference statements)

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“…Rab11 is the master regulator of the recycling endosome, described as a web of tubulovesicular membranes (Mobius et al, 2003), that deliver endocytosed proteins and lipids, as well as material segregated from the trans-Golgi network, to the surface. Given their function, it has been postulated that the recycling endosome transports vRNPs to the surface using the microtubule network (Amorim et al, 2011;Momose et al, 2011). Later, it was shown that several vRNPs colocalize in a Rab11-dependent manner in cytosolic puncta that increase in size with infection, which led to the proposal that assembly of the 8-segment core occurs en route to the surface (Chou et al, 2013;Lakdawala et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it has been shown that IAV vRNPs attach to Rab11 vesicles after nuclear export, and depletion of the two isoforms of Rab11 (Rab11a and Rab11b, henceforward Rab11 refers to both isoforms) impacts negatively on viral production (Amorim et al, 2011;Avilov et al, 2012b;Eisfeld et al, 2011;Momose et al, 2011). Rab11 is the master regulator of the recycling endosome, described as a web of tubulovesicular membranes (Mobius et al, 2003), that deliver endocytosed proteins and lipids, as well as material segregated from the trans-Golgi network, to the surface.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Vale-Costa

Alenquer

Sousa

et al. 2016

Journal of Cell Science

127

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Influenza A virus assembly is an unclear process, whereby individual virion components form an infectious particle. The segmented nature of the influenza A genome imposes a problem to assembly because it requires packaging of eight distinct RNA particles (vRNPs). It also allows genome mixing from distinct parental strains, events associated with influenza pandemic outbreaks. It is important to public health to understand how segmented genomes assemble, a process that is dependent on the transport of components to assembly sites. Previously, it has been shown that vRNPs are carried by recycling endosome vesicles, resulting in a change of Rab11 distribution. Here, we describe that vRNP binding to recycling endosomes impairs recycling endosome function, by competing for Rab11 binding with family-interacting proteins, and that there is a causal relationship between Rab11 ability to recruit family-interacting proteins and Rab11 redistribution. This competition reduces recycling sorting at an unclear step, resulting in clustering of single- and double-membraned vesicles. These morphological changes in Rab11 membranes are indicative of alterations in protein and lipid homeostasis during infection. Vesicular clustering creates hotspots of the vRNPs that need to interact to form an infectious particle.Fundação para a Ciência e Tecnologia grants: (PTDC/IMI-MIC/1142/2012, SFRH/BPD/94204/2013, SFRH/BPD/62982/2009, IF/00899/2013); Fundação Calouste Gulbenkian; Instituto Gulbenkian de Ciência

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Vale-Costa

Alenquer

Sousa

et al. 2016

Journal of Cell Science

127

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“…Some host actin-binding proteins, such as Rab 11 and myosin, are essential for IAV genome transport to the plasma membrane and budding formation [30,31,32,33]. The major actin-depolymerizing factor (ADF)/cofilin isoform, Cofilin-1 is primarily responsible for dynamic actin cytoskeletal reorganization [15], and functions depending on its local concentration of active form relative to actin: relative low concentrations of active cofilin to actin favor severing, whereas high concentrations of cofilin favor nucleating actin assembly [34].…”

Section: Discussionmentioning

confidence: 99%

Cofilin-1 is involved in regulation of actin reorganization during influenza A virus assembly and budding

Liu

Xiang

Guo

et al. 2014

Biochemical and Biophysical Research Communications

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Influenza A virus (IAV) assembly and budding on host cell surface plasma membrane requires actin cytoskeleton reorganization. The underlying molecular mechanism involving actin reorganization remains unclarified. In this study, we found that the natural antiviral compound petagalloyl glucose (PGG) inhibits F-actin reorganization in the host cell membrane during the late stage of IAV infection, which are associated with the suppression of total cofilin-1 level and its phosphorylation. Knock-down of cofilin-1 reduces viral yields. These findings provide the first evidence that cofilin-1 plays an important role in regulating actin reorganization during IAV assembly and budding.

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“…Previous studies demonstrated through the use of live imaging microscopy that, upon entering the cytoplasm, vRNP complexes associate with a pericentriolar recycling endosome marker called Rab11, which is involved in endosomal recycling and trafficking [130]. The accumulation of vRNPs at the microtubule-organizing center after nuclear export, allows them to interact with Rab11-positive recycling endosomes and migrate along microtubules to the sites of budding at the apical surface of the plasma membrane [131][132][133].…”

Section: Unexpected Delivery: Nuclear Export Of Viral Mrna and The Vrnpmentioning

confidence: 99%

Influenza A Virus Multiplication and the Cellular SUMOylation System

Chacon¹,

Rosas-Acosta²

2013

Viral Replication

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A Rab11- and Microtubule-Dependent Mechanism for Cytoplasmic Transport of Influenza A Virus Viral RNA

Cited by 198 publications

References 63 publications

Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Cofilin-1 is involved in regulation of actin reorganization during influenza A virus assembly and budding

Influenza A Virus Multiplication and the Cellular SUMOylation System

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