2020
DOI: 10.1016/j.cell.2020.02.012
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A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging

Abstract: Highlights d A comprehensive and quantitative map of the mouse cysteine redox proteome in vivo d Redox networks are highly tissue selective and underlie tissue-specific biology d Cysteine thiol redox sensitivity is encoded by local electrostatic gating d Identification of redox-modified protein disease networks that remodel in aged mice

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Cited by 274 publications
(343 citation statements)
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“…Unexpectedly, reciprocal reduced and reversibly oxidized catalyst-free Click PEGylation workflows revealed the ATP synthase is substantially (~65%) oxidized before and after fertilization (Figure 4) [116]. To place our findings in context, the median oxidation of thiols in the mammalian proteome is~12% [41] and the Oximouse dataset (see [91]) reveals C244 and C294 are~20% reversibly oxidized. Substantial reversible thiol oxidation is consistent with a distinct subset of thiols being persistently oxidized (≥20%) and tissue specific redox signatures [91].…”
Section: Click Pegylation Is a Useful Tool To Assess Protein Thiol Rementioning
confidence: 88%
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“…Unexpectedly, reciprocal reduced and reversibly oxidized catalyst-free Click PEGylation workflows revealed the ATP synthase is substantially (~65%) oxidized before and after fertilization (Figure 4) [116]. To place our findings in context, the median oxidation of thiols in the mammalian proteome is~12% [41] and the Oximouse dataset (see [91]) reveals C244 and C294 are~20% reversibly oxidized. Substantial reversible thiol oxidation is consistent with a distinct subset of thiols being persistently oxidized (≥20%) and tissue specific redox signatures [91].…”
Section: Click Pegylation Is a Useful Tool To Assess Protein Thiol Rementioning
confidence: 88%
“…Redox proteomic affords hypothesis-free, systematic, and quantitative global thiol proteome profiling [90]. For example, state-of-the-art cysteine reactive phosphate tag technology coupled to tandem mass tag multiplexing identified and quantified 171,000 thiols modification events in mammalian tissues [91] (i.e.,~80% of the total thiol proteome was assessed). Low-throughout immunological techniques (e.g.,~2 thiols per experiment or 0.0009% of the human thiol proteome) may seem redundant when one can quantify the redox state of thousands of protein thiols in parallel in a single experiment.…”
Section: The Case For Using Immunological Techniques To Assess Proteimentioning
confidence: 99%
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“…Several excellent reviews describe genetic variants of TTN and their effects on the etiology and progression of cardiomyopathies [119,120]. In addition, TNN was recently (2020) found to represent a heart-specific protein from the "cysteine redox network" in the Oximouse model [121]. It was demonstrated that TNN is prone to significant modifications via thiol oxidation during aging, leading to cardiomyopathies (for details see [121]).…”
Section: Uorfmentioning
confidence: 99%
“…In addition, TNN was recently (2020) found to represent a heart-specific protein from the "cysteine redox network" in the Oximouse model [121]. It was demonstrated that TNN is prone to significant modifications via thiol oxidation during aging, leading to cardiomyopathies (for details see [121]). To understand whether TTN expression can be controlled by uORFs, Cadar and co-authors investigated the 5 UTR of TTN and identified two uORFs that suppressed TTN translation in non-stressed cardiac HL-1 cell line and primary neonatal rat ventricular myocytes [122].…”
Section: Uorfmentioning
confidence: 99%