A quantitative study of free amino acids in eccrine sweat collected from the forearms of healthy young men during sauna bathing

Abstract

Using ion-exchange column chromatography we measured the free amino acids in eccrine sweat collected from the forearms of 20 healthy young men at intervals of 15, 30, and 45 min during sauna bathing. In comparison with the amino acid concentrations determined after 15 min of sweating (100%) a significant and uniform decrease occurred in the concentrations of all amino acids examined after a further 15 min (65%), with a further decrease after another 15 min (45%). These results indicate that either the mechanism of excretion of amino acids in the secretory cells of the eccrine sweat glands, or the reabsorption of amino acids by the ductular cells, or the combination of both mechanisms are similar for all the amino acids examined here.

The relationship between aerobic fitness level and metabolic profiles in healthy adults

“…Analysis of the targeted metabolomics data revealed significantly different profiles in the fitness groups. Changes in amino acids have been previous reported in eccrine sweat in males , which demonstrated significantly higher concentrations of 14 amino acids in sweat for the untrained men. It is widely known that exercise greatly increases energy expenditure and promotes oxidation of branched chain amino acid (BCAA) .…”

Effect of Regular Sauna on Epidermal Barrier Function and Stratum Corneum Water-Holding Capacity in vivo in Humans: A Controlled Study

“…5). This ionic decrease was also detected in earlier studies on sweat concentration after exercising [19, 20]. The volunteers of the RSG lost a lower amount of NaCl than those in the control group.…”

Direct Laser Writing of the Porous Graphene Foam for Multiplexed Electrochemical Sweat Sensors

“…The collected exercise-induced sweat from the forehead of a healthy individual (Figure S30) shows a decreased UA from 30 to 5 μM and decreased Tyr from 46 to 10 μM during exercise (Figure 5b,c), which can be attributed to the decreased amino acid from increased perspiration. 69 The human subjects with more frequent/intensive weekly physical training also show a lower UA/Tyr value compared with those with less training (Figure S31 and Table S3), as sweat UA and Tyr may be related to fitness condition. 70 This physical training-induced UA/Tyr decrease likely comes from the protective mechanism that the sweat glands reabsorb UA/Tyr from sweat against the continuous loss of essential protein-building substances.…”

“…70 This physical training-induced UA/Tyr decrease likely comes from the protective mechanism that the sweat glands reabsorb UA/Tyr from sweat against the continuous loss of essential protein-building substances. 69 As a product of purine metabolism, the sweat UA level increases after consuming purine-rich foods compared to the subjects with overnight fasting (Figure 5d and Table S4). The accuracy of wearable sensors in analyzing real human sweat samples is validated against high-performance liquid chromatography (HPLC), with a correlation coefficient of 0.943 and 0.953 for UA and Tyr, respectively (Figure S32).…”