A quantitative method for the detection and validation of catalase activity at physiological concentration in human serum, plasma and erythrocytes

Krishna, Honnur; Avinash, Krishnegowda; Shivakumar, Anantharaman; Al-Tayar, Naef Ghllab Saeed; Shrestha, Ashwinee Kumar

doi:10.1016/j.saa.2020.119358

Cited by 4 publications

(4 citation statements)

References 51 publications

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“…The reduced levels of serum glutathione (GSH) were estimated spectrophotometrically at 405 nm and expressed as mg/dL [30]. Serum catalase (CAT) activity was estimated spectrophotometrically at 510 nm and expressed as units/L [31].…”

Section: Evaluation Of Serum Levels Of Oxidative Stress Markersmentioning

confidence: 99%

Barium Oxide Doped Magnesium Silicate Nanopowders for Bone Fracture Healing: Preparation, Characterization, Antibacterial and In Vivo Animal Studies

et al. 2022

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Magnesium silicate (MgS) nanopowders doped with barium oxide (BaO) were prepared by sol-gel technique, which were then implanted into a fracture of a tibia bone in rats for studying enhanced in vivo bone regeneration. The produced nanopowders were characterized using X-ray diffraction (XRD), Fourier transform infrared spectra (FTIR), scanning electron microscope with energy-dispersive X-ray spectrometry (SEM-EDX) and transmission electron microscope (TEM). Mechanical and bactericidal properties of the nanopowders were also determined. Increased crystallinity, particle diameter and surface area were found to decrease after the BaO doping without any notable alterations on their chemical integrities. Moreover, elevated mechanical and antibacterial characteristics were recognized for higher BaO doping concentrations. Our animal studies demonstrated that impressive new bone tissues were formed in the fractures while the prepared samples degraded, indicating that the osteogenesis and degradability of the BaO containing MgS samples were better than the control MgS. The results of the animal study indicated that the simultaneous bone formation on magnesium biomaterial silicate and barium MgS with completed bone healing after five weeks of implantations. The findings also demonstrated that the prepared samples with good biocompatibility and degradability could enhance vascularization and osteogenesis, and they have therapeutic potential to heal bone fractures.

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Section: Evaluation Of Serum Levels Of Oxidative Stress Markersmentioning

confidence: 99%

Barium Oxide Doped Magnesium Silicate Nanopowders for Bone Fracture Healing: Preparation, Characterization, Antibacterial and In Vivo Animal Studies

et al. 2022

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“…Therefore, spectrophotometric measurements are appropriate to deal with fresh samples and samples dried at different temperatures. Several researchers [41,42] have utilized the spectrophotometric measuring approach based on catalase activity in the field to investigate the biological samples.…”

Section: Spectrophotometric Measurementmentioning

confidence: 99%

Catalase Activity in Hot-Air Dried Mango as an Indicator of Heat Exposure for Rapid Detection of Heat Stress

et al. 2022

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The growing market for dried fruits requires more attention to quality parameters. Mango and other tropical fruits are commonly dried at temperatures ranging from 40 °C to 80 °C. Convincing evidence suggests that the nutritional quality of dried fruits is best preserved when dried at low temperatures ≤50 °C, whereas increasing drying temperatures lead to the degradation of the most valuable nutrients inside the fruit. Currently, there is no system or direct measurement method that can assist in identifying the quality deterioration of dried fruits caused by excessive heat exposure during drying. From this perspective, the activity of the heat-sensitive enzyme ‘catalase’ was used for the first time to evaluate and compare mango slices dried at 40 °C, 60 °C and 80 °C. Various methods, including direct and indirect flotation tests and spectrophotometric measurements, were explored to measure the residual catalase activity in the dried samples. Results showed that the spectrophotometry and indirect flotation test produced the best results, revealing a significant difference (p < 0.05) in the catalase activity of mango slices dried at 40 °C, 60 °C and 80 °C, which the direct-dried mango flotation test failed to predict. Furthermore, this study demonstrates the potential applicability of catalase activity to indicate heat stress in dried mango slices processed at different temperatures.

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“…The activity of the SOD was measured in terms of units per milligram of protein [13] . The catalase activity was determined by utilizing a commercial kit (Randox Lab., UK) and measuring it at 340 nm and 37 °C in accordance with the instructions provided by the manufacturer [14] .…”

Section: Determination Of Superoxide Dismutase and Catalase Activitiesmentioning

confidence: 99%

Anti-Inflammatory Activity of Withania Somnifera Root Extract in Complete Freund′S Adjuvant-Induced Arthritis in Male Albino Rats

Khaled¹,

Kamel²,

Darwesh³

et al. 2022

The Egyptian Journal of Hospital Medicine

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Background: A very high rate of morbidity and mortality is linked to the condition known as rheumatoid arthritis (RA). Objective: The current study has aimed to examine the impact of daily delivery of Withania somnifera (W. somnifera) or Ashwagandha extract (ASHE) and standard drug (Diclofenac potassium) on oxidative stress and inflammatory biomarker in adjuvant-induced arthritis (AIA) in male albino rats. Materials and methods: Animals were divided into ten groups (n=5). Group 1 acted as control, group 2 presented the RA rats and groups 3, 4, 5 were treated with different doses of ASHE (50, 100 and 200 mg/kg, respectively) daily for 3 weeks, Group 6 received standard drug (Diclofenac potassium) at a daily dose of 10 mg/kg for 3 weeks. Groups 7, 8 and 9 represent CFA-induced RA rats, which received tested extract at 50, 100 and 200 mg/kg respectively: daily for 3 weeks. The tenth group represented RA group received standard drug (Diclofenac potassium). Results: The changes induced in AIA rats were evaluated by measurement of oxidant and antioxidant biomarkers as well as interleukins-1β (IL-1β) and tumor necrosis factor alpha (TNF-α), in addition to cyclooxygenase-1 (COX1) and cyclooxygenase-2 (COX2) measurement. W. somnifera dose-dependently alleviated the severity of the disease based on the reduction of lipid peroxidation and ceruloplasmin and improved the GSH, SOD, CAT and TAC as compared to AIA-rats. ASHE reduced the serum level of IL1β, TNF, as well as COX1 and COX2 as compared to AIA-rats. Conclusion:The results of the experiments showed that W. somnifera might be useful for management of RA.

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A quantitative method for the detection and validation of catalase activity at physiological concentration in human serum, plasma and erythrocytes

Cited by 4 publications

References 51 publications

Barium Oxide Doped Magnesium Silicate Nanopowders for Bone Fracture Healing: Preparation, Characterization, Antibacterial and In Vivo Animal Studies

Barium Oxide Doped Magnesium Silicate Nanopowders for Bone Fracture Healing: Preparation, Characterization, Antibacterial and In Vivo Animal Studies

Catalase Activity in Hot-Air Dried Mango as an Indicator of Heat Exposure for Rapid Detection of Heat Stress

Anti-Inflammatory Activity of Withania Somnifera Root Extract in Complete Freund′S Adjuvant-Induced Arthritis in Male Albino Rats

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