A quantitative method for the determination of the specific radioactivity of sulfur-containing amino acids separated by paper chromatography

Awwad, Hassan K.; Adelstein, S. James

doi:10.1016/0003-2697(66)90225-9

Cited by 43 publications

(15 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection of sulfur-containing compounds using hexaiodoplatinate is based on optical changes following the substitution of the coordination bonds between platinum atoms and iodide atoms with sulfur atoms (21). In the past, several investigators have applied this principle to the HPLC analysis of sulfur-containing amino acids in infusion fluid (23) or an ACE inhibitor and its metabolites in urine (22).…”

Section: Discussionmentioning

confidence: 99%

“…However, such methods are often time consuming and require extremely careful sample handling, in order to avoid degradation of derivative products and/or the formation of by-products (18). Fortunately, the hexaiodoplatinate molecule, a sulfur-specific detection reagent, contains iodide atom(s) which are able to exchange with sulfur atom(s), resulting in the formation of measurable chromophores (21). Thus, it is possible to apply this reagent to postcolumn reactive high-performance liquid chromatography (HPLC) because of its rapid substitution reaction and spectroscopic characteristics.…”

Section: N-acetyl-l-cysteine (Nac)mentioning

confidence: 99%

See 1 more Smart Citation

Determination of Reduced, Protein-Unbound, and Total Concentrations of N-Acetyl--cysteine and -Cysteine in Rat Plasma by Postcolumn Ligand Substitution High-Performance Liquid Chromatography

Harada

Naito

Kawauchi

et al. 2001

Analytical Biochemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: N-acetyl-l-cysteine (Nac)mentioning

confidence: 99%

Determination of Reduced, Protein-Unbound, and Total Concentrations of N-Acetyl--cysteine and -Cysteine in Rat Plasma by Postcolumn Ligand Substitution High-Performance Liquid Chromatography

Harada

Naito

Kawauchi

et al. 2001

Analytical Biochemistry

View full text Add to dashboard Cite

“…Quantification was carried out by standard curve equations, using homarine and glycine-betaine (Merck) as standard. 3-Dimethylsulphoniopropionate (DMSP) was separated on 0.25 mm silica gel G plates (Machery-Nagel, Du¨ren, Germany) developed with methanol:acetone:HCl (90:10:4, v/v/v) (Summers et al, 1998), and visualized by Dragendorff's reagent according to the method of Awwad & Adelstein (1966). DMSP was estimated by area measurement of Dragendorff-positive TLC zones (Aronoff, 1967).…”

Section: Biochemical Analysismentioning

confidence: 99%

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Scholz

Liebezeit

2012

European Journal of Phycology

View full text Add to dashboard Cite

Diatoms inhabiting intertidal flats are subject to strongly changing salinities due to exposure to rain and desiccation at low tide. In order to determine the physiological responses of marine benthic diatoms to salinity changes, cultures of Navicula phyllepta, Achnanthes delicatula subsp. hauckiana and Nitzschia constricta, isolated from the Soltho¨rn tidal flat (lower Saxony, southern North Sea), were used to study the effect on growth rates of different salinities (0.5, 10, 20, 30, 40, 50). During short (1, 3, 10, 60 min) and long exposures (30 days), the composition of free amino acids and the accumulation of polyols, saccharides, quaternary ammonium compounds and -dimethylsulphoniopropionate (DMSP) were determined. The growth rates of N. phyllepta were not affected by salinity in the tested range, synthesizing and accumulating proline as the only response to salinity increases in the growth medium. The growth responses of A. delicatula subsp. hauckiana were significantly weaker at the lowest and highest salinities in comparison to the control treatment (at a salinity of 30), producing proline, glycine betaine, glycerol and the quaternary ammonium compound homarine. In contrast, N. constricta showed the highest growth rate in the low-salinity treatment and the lowest proline concentrations in the high-salinity assay in comparison to the other species. In addition, N. constricta was the only species that synthesized the tertiary sulphonium compound DMSP. Furthermore, results from the salinity treatments showed particular similarities in compound accumulation. While quaternary ammonium compounds as well as DMSP were synthesized during high-salinity treatments, polyols and carbohydrates were found predominantly in the low-salinity experiments. Enzyme activities specific to proline metabolism in A. delicatula and N. constricta indicated that the high salinity-induced free proline accumulation may be due to a stimulation of synthesis via the ornithine pathway, whereas in N. phyllepta the glutamate pathway may contribute significantly to the free proline accumulation. In conclusion, significant differences in osmolyte compositions were found in the three diatom species during exposure to different salinities, suggesting specific intracellular acclimation processes that provide possible explanations of the species' insensitivity towards environmental short-time salinity variations.

show abstract

“…DMSP was separated on 0.25 mm silica gel G plates (Machery-Nagel, Düren, Germany) developed with methanol: acetone: concentrated HCl (90 : 10 : 4, v/v/v) (Summers et al, 1998), and visualized using Dragendorff reagent according to Awwad & Adelstein (1966). DMSP was estimated by area measurement of Dragendorff-positive TLC zones (Aronoff, 1967).…”

Section: Dimethylsulfoniopropionate (Dmsp)mentioning

confidence: 99%

Effects of UV radiation on five marine microphytobenthic Wadden sea diatoms isolated from the Solthörn tidal flat (Lower Saxony, southern North Sea) – Part I: growth and antioxidative defence strategies

Scholz¹,

Rúa²,

Liebezeit³

2014

European Journal of Phycology

View full text Add to dashboard Cite

A quantitative method for the determination of the specific radioactivity of sulfur-containing amino acids separated by paper chromatography

Cited by 43 publications

References 4 publications

Determination of Reduced, Protein-Unbound, and Total Concentrations of N-Acetyl--cysteine and -Cysteine in Rat Plasma by Postcolumn Ligand Substitution High-Performance Liquid Chromatography

Determination of Reduced, Protein-Unbound, and Total Concentrations of N-Acetyl--cysteine and -Cysteine in Rat Plasma by Postcolumn Ligand Substitution High-Performance Liquid Chromatography

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Effects of UV radiation on five marine microphytobenthic Wadden sea diatoms isolated from the Solthörn tidal flat (Lower Saxony, southern North Sea) – Part I: growth and antioxidative defence strategies

Contact Info

Product

Resources

About