A quantitative immunosensing technique based on the measurement of nanobeads’ Brownian motion

Fan, Yuanyuan; Sheen, Horn‐Jiunn; Hsu, Chia-Jui; Liu, Cheng Pang; Lin, Shiming; Wu, Kuang‐Chong

doi:10.1016/j.bios.2009.07.037

Cited by 22 publications

(27 citation statements)

References 19 publications

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“…5a. The results exhibit that the variations of Brownian velocities are similar to those observed in previous results, in which the transition and the equilibrium states for the antibody-coated nanobeads and CRP could be clearly distinguished (Fan et al 2009). …”

Section: Resultssupporting

confidence: 86%

“…Particle recognition in the images was accomplished by setting a threshold, which is a fraction of the peak image intensity, and particle size sorting. The velocities of the particles can be quantified by the PTV algorithm described by Fan et al (2009), which means that the Brownian velocities can be evaluated by statistically analyzing the particle velocities. Furthermore, 3D-TIRFM technology enables the distances between the nanobeads and the boundary to be evaluated by their own intensities.…”

Section: Methodsmentioning

confidence: 99%

“…The characterization of such high spatial resolution in the vertical direction from the surface can be implemented to distinguish the various elevations of the particles. 3D TIRFM can be used to describe the vertical distribution of the particles in various electrolytes by analyzing one evanescent wave illumination image to establish a 3D profile of the microchannel boundary flow (Fan et al 2009) and to measure the 3D Brownian motion of a single or multiple particles in the near-wall region (Huang and Breuer 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The interaction of the two beads was observed in real time through the variation of their Brownian motions. Another approach of measurement Brownian motion of antibody-coated nanobead interacting with antigens was developed (Fan et al 2009;Gorti et al 2008). By statistically analyzing Brownian diffusion decay in a population of antibody-coated nanobeads in a particular period, the concentration of antigens in the liquid solution can be determined.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Fan

Sheen

Chen

et al. 2015

Microfluid Nanofluid

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Section: Resultssupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Fan

Sheen

Chen

et al. 2015

Microfluid Nanofluid

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“…At constant temperature and liquid viscosity, the diffusion coefficient is simply a function of the particle diameter. Techniques based on measuring Brownian motion are used to detect M13 viruses26 and C-reactive protein27. Their result shows that the Brownian motion of particles decreases when the particles are bound with target analytes2627.…”

mentioning

confidence: 99%

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Chung

Wang

Chuang

2017

Sci Rep

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Successful treatments against bacterial infections depend on antimicrobial susceptibility testing (AST). However, conventional AST requires more than 24 h to obtain an outcome, thereby contributing to high patient mortality. An antibiotic therapy based on experiences is therefore necessary for saving lives and escalating the emergence of multidrug-resistant pathogens. Accordingly, a fast and effective drug screen is necessary for the appropriate administration of antibiotics. The mixed pathogenic nature of infectious diseases emphasizes the need to develop an assay system for polymicrobial infections. On this basis, we present a novel technique for simultaneous and quantitative monitoring of co-cultured microorganisms by coupling optical diffusometry with bead-based immunoassays. This simple integration simultaneously achieves a rapid AST analysis for two pathogens. Triple color particles were simultaneously recorded and subsequently analyzed by functionalizing different fluorescent color particles with dissimilar pathogen-specific antibodies. Results suggested that the effect of the antibiotic, gentamicin, on co-cultured Pseudomonas aeruginosa and Staphylococcus aureus was effectively distinguished by the proposed technique. This study revealed a multiplexed and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacterial count (50 CFU per droplet, ~105 CFU/mL) for continuously monitoring the growth of co-cultured microorganisms. This technique provides insights into timely therapies against polymicrobial diseases in the near future.

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Enhancing the sensitivity of portable biosensors based on self-powered ion concentration polarization and electrical kinetic trapping

et al. 2020

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A quantitative immunosensing technique based on the measurement of nanobeads’ Brownian motion

Cited by 22 publications

References 19 publications

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Enhancing the sensitivity of portable biosensors based on self-powered ion concentration polarization and electrical kinetic trapping

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