A quantitative diagnostic method for oral mucous precancerosis by Rose Bengal fluorescence spectroscopy

Abstract

A novel in vivo fluorescence spectroscopic diagnostic method has been developed in an animal model to make a quantified precancer diagnosis. In the study, 40 golden hamsters were randomly divided into four groups (groups A, B, C, and D), with group A being the control group and the other three groups being inducted at different precancer stages. A 1% Rose Bengal (RB) solution was used for the fluorescence spectroscopic diagnosis. A parameter K defined as K = I(RB)/I(auto) was introduced to reflect the amount of RB in the tissue, where I(RB) and I(auto) represent the fluorescence peak intensity of the RB in the tissue and the autofluorescence intensity of tissue at 580 nm, respectively. The average K values of the four groups were calculated and statistically analyzed by analysis of variance (ANOVA), which revealed statistically significant differences within each group as well as between groups (p< 0.001). After analysis by Clementine 11.1 C&R Tree modeling (CART), the following diagnostic criteria were set: normal, K ≤ 8.91; simple hyperplasia, 8.91 < K ≤ 41.92; mild dysplasia, 41.92 < K ≤ 70.79; moderate and severe dysplasia, K >70.79. The sensitivity and specificity to detect precancerous lesions compared with scalpel biopsy were calculated. The results of this study showed that the spectrofluorometric method mediated by RB could accurately discriminate different precancer stages.

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

“…Rose bengal has several applications in medicine that include the diagnosis of ocular damage, the detection of oral pre-cancerous lesions and the use as a dental plaque disclosing agent [6,7]. The combinational use of rose bengal and blue-light has recently gained interest to inactivate several oral pathogens causing dental and periodontal diseases [8,9,10].…”

Non-Invasive Techniques for Detection and Diagnosis of Oral Potentially Malignant Disorders

“…Two studies demonstrated that RB staining may be a valuable diagnostic technique in the detection of oral PMDs and oral cancer (Du et al 2007;Mittal et al 2012). In one study, authors investigated the efficacy of RB staining in the detection of oral PMDs and (Warnakulasuriya and Johnson 1996;Epstein et al 1997;Ram and Siar 2005;Epstein et al 2008;Lingen et al 2008;Patton et al 2008;Balevi 2011;Awan et al 2012;Mojsa et al 2012;Rajmohan et al 2012) Methylene blue staining 90-91.4 66.6-69 (Chen et al 2007a;Chen et al 2007b;Riaz et al 2013) Rose bengal staining 90-100 73.7-89.09 (Du et al 2007;Zhang et al 2013) Lugol's iodine staining 87.5-94.7 83.8-84.2 (Epstein et al 1992;Chaudhari et al 2013) Light-based detection systems…”

“…Zhang et al (2013) combined fluorescence spectroscopic techniques with RB staining to detect oral PMDs in an animal model in vivo and reported that the method showed excellent sensitivity. Recently, a rose-bengal-conjugated gold nanorod (RB-GNR) platform was developed for the optical detection of cancer cells (Wang et al 2014).…”

A Hypercoagulable Hematological Metastasis Breast Cancer Model

“…Given the mechanism underlying the hypercoagulable status, which is characterized by platelet activation and contributes to breast cancer metastasis, has not been fully revealed. As an efficient and stable model is recognized as a key basis for research [28], which is contributed to explore the underlying mechanism of that, we committed to constructing in vivo and in vitro models in this field. In this study, by constructing and comprehensively evaluating the effectiveness of in vivo and in vitro models, we provide powerful research tools to study the hypercoagulable status that is characterized by platelet activation in the context of promoting hematogenous metastasis in breast cancer.…”