A quantitative approach to measure joint pain in experimental osteoarthritis—evidence of a role for nitric oxide

Castro, Rondinelle Ribeiro; Cunha, Fernando; Silva, F.S.; Rocha, Francisco Airton Castro

doi:10.1016/j.joca.2006.01.013

Cited by 50 publications

(42 citation statements)

References 43 publications

(52 reference statements)

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“…The induction of OA was performed using the method as previously described [20,21] . Briefly, each animal was anesthetized using a mixture of xylazine (5 mg/kg, Thai Meiji Pharmaceutical Co., Ltd., Bangkok, Thailand) and zoletil (40 mg/kg, Virbac Laboratories, Carros, France) administered by a single intraperitoneal (i.p.)…”

Section: Induction Of Oa: Aclt Modelmentioning

confidence: 99%

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Morus alba L. Stem Extract Attenuates Pain and Articular Cartilage Damage in the Anterior Cruciate Ligament Transection-Induced Rat Model of Osteoarthritis

Khunakornvichaya

Lekmeechai²,

Pham³

et al. 2016

Pharmacology

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Aim: This study was designed to investigate the anti-nociceptive effect of Morus alba stem extract as well as its cartilage protective effect in the anterior cruciate ligament transection (ACLT)-induced rat model of osteoarthritis (OA). Methods: The anti-nociceptive effect of this plant extract was determined by measuring hind limb weight bearing, while the severity of cartilage damage to the knee joints was evaluated using the modified Mankin grading system. Results: Oral administration of M. alba stem extract (56 and 560 mg/kg) significantly attenuated joint pain as indicated by a significant (p < 0.05) increase in the values of percent weight borne on the operated hind limb for the OA-induced groups that received M. alba stem extract at 56 and 560 mg/kg when compared to those of the vehicle-treated OA-induced group. In addition, a significant improvement in the Mankin score was also observed in rats treated with 560 mg/kg M. alba stem extract, which was in agreement with its pain-relieving effect. Conclusion: The results showed that M. alba stem extract exhibited an anti-nociceptive effect as well as cartilage protection in the ACLT-induced rat model of OA, supporting its potential use as a therapeutic treatment for OA.

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Section: Induction Of Oa: Aclt Modelmentioning

confidence: 99%

“…injection. For OA animals, the ACL was transected and confirmed by an anterior drawer test [20] . Sham-operated animals underwent the same surgery as OA animals, except that transection of the ACL was omitted.…”

Section: Induction Of Oa: Aclt Modelmentioning

confidence: 99%

Morus alba L. Stem Extract Attenuates Pain and Articular Cartilage Damage in the Anterior Cruciate Ligament Transection-Induced Rat Model of Osteoarthritis

Khunakornvichaya

Lekmeechai²,

Pham³

et al. 2016

Pharmacology

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“…Osteoarthritis (OA) is one of the most common joint disease in humans and animals and is related to the deterioration of articular cartilage, subchondral bone and/or synovial membrane, leading to failure of the synovial joint (Amiel et al 2003, Castro et al 2006. In the early stages of OA, the lesions may be focused on some of the femorotibial joint compartment, among them the most common is the femoral condyles (Kamei, Sumen & Sakaridani 2008).…”

Section: Introductionmentioning

confidence: 99%

Experimental osteoarthritis in rabbits: lesion progression

et al. 2013

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ABSTRACT.-Campos W.N.S., Souza M.A., Ruiz T., Peres T.P., Néspoli P.B., Marques A.T.C., Colodel E.M. & Souza R.L. 2013. Experimental osteoarthritis in rabbits: lesion progression. Pesquisa Veterinária Brasileira 33(3):279-285. Programa de Pós-Graduação em Ciên-cias Veterinárias, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa 2367, Bairro Boa Esperança, Cuiabá, MT 78068-900, Brazil. E-mail: wilmavet@gmail.comThe aim of this study was to evaluate the progression of lesions in different stages of osteoarthritis (OA) experimental by radiography (RX), computed tomography (CT), macroscopic and histopathology, linking these different diagnostic methods, helped to provide information that helps the best time for the therapeutic approach. Four experimental periods were delineated at 3, 6, 9 and 12 weeks after induction of OA, known as PI, PII, PIII and PIV, respectively, each with six animals. We evaluated the five compartments of the femorotibial joint: medial femoral condyle (MFC), lateral femoral condyle (LFC), medial tibial plateau (MTP), lateral tibial plateau (LTP) and femoral trochlea (FT). Therefore we established an index by compartment (IC) and by adding such an index was estimated joint femorotibial (IFT). It was observed that the CFM was the compartment with the highest IC also differed significantly (p<0.05) from other compartments. Compartments showed no significant difference (p>0.05) between the PI and PII, however contrary fact occurred between the PII and PIII (p<0.05), PIII and PIV (p<0.01) and between PI and PIV (p<0.001). Similarly the IFT, showed a significant difference in the animals of PIV compared to PI (p<0.001), PII (p<0.001) and PIII (p<0.01), and there was no statistical difference (p> 0.05) between the PI and PII. In the variation of the average interval between periods, there was a higher value between the PIII PIV and for the other intervals of time periods (PI, PII, and PIII-PII). However, these intervals showed no statistically significant difference (p>0.05). Through the RX, CT, macroscopic and histopathological findings, we found similar patterns among individuals within the same period demonstrating a gradual progression of the disease. These results show that between 3 and 6 weeks progression of the lesion is slower and probably also can be reversed in comparison to other ranges where proved further progression between 9 and 12 weeks after induction of trauma OA. These results may provide a better therapeutic approach aimed at reversing the lesions in early stages of OA. We conclude that the interconnection of the four diagnostic methods individually classified into scores, which were unified in both indices in the evaluation by the femorotibial joint compartment and may represent a diagnostic condition closer to the true condition of the injury and its progression. . Através do RX, TC, macroscopia e histopatológico, verificamos padrões similares entre os indivíduos dentro do mesmo período demonstrando assi...

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“…• при артрозе под действием провоспалительных цитокинов (Castro et al, 2006). Следует отметить, что iNOS, преимущественно, регулируется на уровне транскрипции, при этом промо-тор гена iNOS содержит сайты связывания многих транс-крипционных факторов: АР-1, CREB, HIF, NF-kB и др., в том числе, индуцируемых IL-1β и TNFα (Pautz et аl., 2010).…”

Section: результаты и обсуждениеunclassified

The association between no-synthases gene polymorphisms and post-traumatic knee ostearthritis development among Rostov region residents

Vnukov

Krolevets

Panina

et al. 2015

Ecological genetics

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Background. Knee osteoarthritis (OA) is a common chronic degenerative disease. The risk factors for the development of knee OA are joint trauma and genetic predisposition. The aim of the present work was to study the associations between gene polymorphisms -84G A nNOS and -786T C eNOS and the risk of post-traumatic knee osteoarthritis (PTOA) development among Rostov region residents and to define the interactions between these polymorphisms and nitrite level in the plasma and synovial fluid (SF) and chondrocyte apoptosis in patients with PTOA. Materials and Methods. Case-control study involved 117 post-traumatic knee OA patients and 94 healthy controls. The nNOS and eNOS polymorphisms were determined by polymerase chain reaction. The nitrite concentrations in the plasma and synovial fluid were defined using Griess reaction. Chondrocyte apoptosis in cartilage sections was assessed morphologically by electron microscopy. Results. The G-84A nNOS genotype showed statistical differences between groups. The A allele is associated with PTOA development (OR 2,02, CI 1,08-3,76, p = 0,02). The eNOS genotype did not show statistical difference between groups. The NOx- concentration was higher both in plasma (p = 0,022) and synovial fluid (p = 0,014) of PTOA patients compared with plasma of healthy subjects. Multiple regression analyses demonstrated that the nitrite level in the SF is linked with -786T C NOS3 genotype; the -786С allele leaded to the decreasing in NOx- concentration. The electron microscopy confirmed the presence of early and late apoptotic chondrocytes in PTOA cartilage tissue. Conclusion. The G-84A nNOS polymorphism may be associated with PTOA development and chondrocyte apoptosis intensification. The -786С allele of -786T C NOS3 is associated with reduced NOx- concentration in the synovial fluid.

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A quantitative approach to measure joint pain in experimental osteoarthritis—evidence of a role for nitric oxide

Abstract: We describe a method to quantitate joint pain associated to weight bearing in the ACLT model. The joint pain is sensitive to classical antinociceptive compounds. NO release is associated to joint pain though NOS inhibition does not inhibit ongoing pain.

Cited by 50 publications

References 43 publications

<b><i>Morus alba</i></b> L. Stem Extract Attenuates Pain and Articular Cartilage Damage in the Anterior Cruciate Ligament Transection-Induced Rat Model of Osteoarthritis

<b><i>Morus alba</i></b> L. Stem Extract Attenuates Pain and Articular Cartilage Damage in the Anterior Cruciate Ligament Transection-Induced Rat Model of Osteoarthritis

Experimental osteoarthritis in rabbits: lesion progression

The association between no-synthases gene polymorphisms and post-traumatic knee ostearthritis development among Rostov region residents

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