A quantitative approach for Cd, Cu, Fe and Mn through laser ablation imaging for evaluating the translocation and accumulation of metals in sunflower seeds

Pessôa, Gustavo de Souza; Júnior, Cícero Alves Lopes; Madrid, Katherine Chacón; Arruda, Marco Aurélio Zezzi

doi:10.1016/j.talanta.2017.02.029

Cited by 36 publications

(12 citation statements)

References 45 publications

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“…In order to understand the ways of distribution and transport of elements in plant tissues, different techniques of elemental imaging are used, starting from microscopic techniques through techniques based on mass spectrometry and ending with synchrotron X-ray-based techniques [30]. However, undoubtedly, techniques based on mass spectrometry, such as LA-ICP-MS offer a range of advantages in high detection limits and high sensitivity for many elements [31,32]. The coupling of a laser with an ICP-MS as a detector, gives the possibility of wide-range of imaging analysis with good spatial resolution, high sensitivity and capability to image/locate many elements in a single analysis of plant samples [33,34], providing unique data that renders LA-ICP-MS a powerful technique in bioimaging.…”

Section: Introductionmentioning

confidence: 99%

Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

et al. 2019

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In this study, for a first time (according to our knowledge), we couple the methodologies of chlorophyll fluorescence imaging analysis (CF-IA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), in order to investigate the effects of cadmium (Cd) accumulation on photosystem II (PSII) photochemistry. We used as plant material Salvia sclarea that grew hydroponically with or without (control) 100 μM Cd for five days. The spatial heterogeneity of a decreased effective quantum yield of electron transport (ΦPSΙΙ) that was observed after exposure to Cd was linked to the spatial pattern of high Cd accumulation. However, the high increase of non-photochemical quenching (NPQ), at the leaf part with the high Cd accumulation, resulted in the decrease of the quantum yield of non-regulated energy loss (ΦNO) even more than that of control leaves. Thus, S. sclarea leaves exposed to 100 μM Cd exhibited lower reactive oxygen species (ROS) production as singlet oxygen (1O2). In addition, the increased photoprotective heat dissipation (NPQ) in the whole leaf under Cd exposure was sufficient enough to retain the same fraction of open reaction centers (qp) with control leaves. Our results demonstrated that CF-IA and LA-ICP-MS could be successfully combined to monitor heavy metal effects and plant tolerance mechanisms.

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Section: Introductionmentioning

confidence: 99%

Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

et al. 2019

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“…In addition, for NP imaging and quantification in biological samples, it is necessary to use appropriate calibration approaches. Important challenges remain not only due to the absence of a variety of suitable reference materials for calibration but also in the selection of the best internal standard that helps compensate for laser fluctuations, differences in sample thickness, the sample mass ablated and transported, and instabilities and drifts of the ICP source . In this context, Böhme et al applied AuNPs, AgNPs, and Al 2 O 3 ‐NPs, of different particle sizes, in agarose gels, used as a model for soft matrices.…”

Section: Nanoparticles and Elemental Distribution Using La‐icp‐msmentioning

confidence: 99%

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Galazzi

Chacón-Madrid

Freitas

et al. 2020

Rapid Comm Mass Spectrometry

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The widespread application of nanoparticles (NPs) in recent times has caused concern because of their effects in biological systems. Although NPs can be produced naturally, industrially synthesized NPs affect the metabolism of a given organism because of their high reactivity. The biotransformation of NPs involves different processes, including aggregation/agglomeration, and reactions with biomolecules that will be reflected in their toxicity. Several analytical techniques, including inductively coupled plasma mass spectrometry (ICP‐MS), have been used for characterizing and quantifying NPs in biological samples. In fact, in addition to providing information regarding the morphology and concentration of NPs, ICP‐MS‐based platforms, such as liquid chromatography/ICP‐MS, single‐particle ICP‐MS, field‐flow fractionation (asymmetrical flow field‐flow fractionation)‐ICP‐MS, and laser ablation‐ICP‐MS, yield elemental information about molecules. Furthermore, such information together with speciation analysis enlarges our understanding of the interaction between NPs and biological organisms. This study reports the contribution of ICP‐MS‐based platforms as a tool for evaluating NPs in distinct biological samples by providing an additional understanding of the behavior of NPs and their toxicity in these organisms.

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“…colour, resolution, and 2D/3D visualization) and convinces the user by its easy specification of data acquisition parameters (ablation speed time, acquisition time) and data line positions (Fig. 5) [39]. The LA-iMageS application has a clear layout for elemental data extraction, data visualization, and data export.…”

Section: La-imagesmentioning

confidence: 99%

Software solutions for evaluation and visualization of laser ablation inductively coupled plasma mass spectrometry imaging (LA-ICP-MSI) data: a short overview

Weiskirchen

Kim

et al. 2019

J Cheminform

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Mass spectrometry imaging (MSI) using laser ablation (LA) inductively coupled plasma (ICP) is an innovative and exciting methodology to perform highly sensitive elemental analyses. LA-ICP-MSI of metals, trace elements or isotopes in tissues has been applied to a range of biological samples. Several LA-ICP-MSI studies have shown that metals have a highly compartmentalized distribution in some organs, which might be altered in consequence of genetic diseases, intoxication, or malnutrition. Although metal imaging by LA-ICP-MSI is an established methodology, potential pitfalls in the determination of metal concentrations might result from erroneous calibration, standardization, and normalization. In addition, for simple display of final imaging results, most LA-ICP-MSI users prefer to process their measurements by commercial processing software. Such programs typically visualize the regional metal differences in colorful and vivid imaging maps, but might not represent the actual signal densities correctly. There is a great abundance of such MSI data processing programs available differing in quality, usability, integrated features, workflow, reliability, system requirements, speed of data processing, and price. Some software packages contain a multitude of features which are superfluous for most users. In contrast, often only few data formats are used, in case of commercial programs even only the instrument provider’s own raw data format. Therefore, first time and average users are often confused and helpless in choosing the correct software for processing their data. Here we have briefly summarized software packages, data routines, macros, programming tools, scripts, algorithms, or self-written patches and updates for existing programs presently in use for mining LA-ICP-MSI data.

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A quantitative approach for Cd, Cu, Fe and Mn through laser ablation imaging for evaluating the translocation and accumulation of metals in sunflower seeds

Cited by 36 publications

References 45 publications

Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Software solutions for evaluation and visualization of laser ablation inductively coupled plasma mass spectrometry imaging (LA-ICP-MSI) data: a short overview

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