A Quantitative and Qualitative Study of Rat Testis Following Administration of Methadone and Buprenorphine

Heidari, Zahra; Mahmoudzadeh‐Sagheb, Hamidreza; Kohan, Farhad

doi:10.5812/ijhrba.4119

Cited by 22 publications

(21 citation statements)

References 22 publications

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“…They reported that these drugs have effects on spermatogenesis and secondary sex organs. In addition, opiates could affect testes volume and induce arrested spermatogenesis, sloughed germinal epithelium, destroyed Sertoli cells, and thickened and irregular basement membrane as well as signs of apoptosis [63]. Also, another study revealed that the testes undergo severe diffused testicular degeneration with numerous spermatocyte and spermatid giant cell formation without spermatogenesis after tramadol treatment.…”

Section: Discussionmentioning

confidence: 99%

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Ibrahim

Salah-Eldin

2019

Pharmacology

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Aim: The present study aimed to elucidate the effects of tramadol on the testicular functions of adult male rats due to the chronic usage of tramadol and the effect of its withdrawal. Method: Adult male albino rats were classified into the following 3 groups: (I) a control administered with normal saline and (II) tramadol-treated rats (40 mg/kg b.w. orally) for 21 successive days; and (III) like the rats in the second group but kept for 4 weeks after the last tramadol dose to study the effect of tramadol withdrawal. At the end of the experimental period, blood was collected and specimens from testis were taken for histopathological, biochemical, and molecular studies. A reverse transcription-polymerized chain reaction after RNA extraction from specimens was detected for the anti-apoptotic and pro-apoptotic genes in testicular tissues. Also, malondialdehyde (MDA) was measured in tissues homogenate and antioxidant enzymes activities were evaluated. Results: The results of this study demonstrated histological changes in testicular tissues in groups II and III compared to the control group, accompanied with increased apoptotic index and proved by increased B-cell lymphoma-2 (Bcl-2) associated-X-protein and caspase-3 expression, whereas anti-apoptotic Bcl-2 markedly decreased. Moreover, in tramadol-abused and -withdrawal groups, the MDA level increased, while the antioxidant enzymes activity decreased and revealed oxidative stress, indicating that tramadol is harmful at the cellular level and can induce apoptotic changes in testicular tissues. The withdrawal effect showed signs of improvement, but it did not return to normal levels. Conclusions: It could be concluded that the administration of tramadol causes abnormalities on testicular tissues associated with oxidative stress, which confirmed the risk of increased oxidative stress on testicular tissues due to tramadol abuse.

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Section: Discussionmentioning

confidence: 99%

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Ibrahim

Salah-Eldin

2019

Pharmacology

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“…In addition to opiate induced reduction of serum testosterone level; there is abnormal structural and functional abnormalities of the secondary sex organs. It seems that probably opiates could affect testicular volume and induce arrested spermatogenesis, sloughed germinal epithelium, destroyed Sertoli cells and thickened and irregular basement membrane as well as signs of apoptosis [26].…”

Section: Discussionmentioning

confidence: 99%

Histopathological and Biochemical Effects of Acute & Chronic Tramadol drug Toxicity on Liver, Kidney and Testicular Function in Adult Male Albino Rats

2016

FRCIJ

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Background: Tramadol is becoming abused among teens; especially between males. The study aimed to investigate the histopathological and biochemical profiles of acute and chronic toxic effects of tramadol on hepatic, renal and testicular functions.Methodology: Sixty male adult albino Sprague-Dawley rats were used. Rats were divided into three groups. Group I: control. Group II: representing acute tramadol toxicity and group III: representing tramadol dependent use for 60 days.Results: Group II displayed hemorrhage and cytolysis in the hepatocytes. Group III showed complete cell membrane degeneration of hepatocytes. Renal tissues revealed glomerular hemorrhage in group II and atrophied glomerulus with collapsed tufts, degenerated tubules and cellular infiltration in group III. The testicular tissues revealed atrophy of seminiferous tubules with interstitial calcification in group II. Focal testicular degeneration, with a little evidence of spermatogenesis in group III. Biochemical indices showed significant increase in liver enzymes, serum bilirubin, creatinine and blood urea nitrogen levels. The sex hormones levels were significantly increased for estradiol and prolactin, while there was a significant decrease in testosterone with a gradual reduction in luteinizing and follicular stimulating hormones.Recommendations: Designing a national awareness campaign to the youth to spotlights on the health hazards of tramadol abuse.

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“…Some researchers reported that reduction in the diameter and epithelium height of seminiferous tubules may be because of a decrease in the number of type A spermatogonia, spermatocyte, spermatid and Sertoli cells (Mohammad‐Ghasemi et al ., ; Soleimani‐Mehranjani et al ., ). The results of our study are in accordance with the one recently showed that methadone administration prevented spermatogenesis, sloughed (degenerated) the germinal epithelium in seminiferous tubules of rat testis (Heidari et al ., ). Therefore we suppose that Kerack administration may reduce diameter of germinal epithelium in the seminiferous tubules.…”

Section: Discussionmentioning

confidence: 97%

Correlation between expression of CatSper family and sperm profiles in the adult mouse testis following Iranian Kerack abuse

et al. 2014

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SUMMARYIllicit drug use can be an important cause of male infertility. The aim of this study was to investigate the effects of an Iranian illicit drug, Kerack, on sperm parameters, testicular structure and CatSper genes expression of mice. In this study, 25 male mice were divided into five groups consisting of control, sham and three experimental groups. All animal in experimental groups were addicted to Kerack for 7 days. These experimental groups include experimental I which was given Kerack at a dose of 5 mg/kg, experimental II, 35 mg/kg and experimental III, 70 mg/kg, intraperitoneally twice a day for a period of 35 days. Mice were then sacrificed and spermatozoas were removed from cauda epididymis and analyzed for count, motility, morphology (normal/abnormal) and viability. Right testes were removed, weighed and processed for light microscopic studies whereas left testes removed were subjected to total mRNA extraction for using in real-time PCR (RT-PCR). The results were analyzed by performing ANOVA (Tukey's tests) and Pearson correlation coefficient. Sperm parameters and seminiferous epithelium thickness were decreased in experimental groups (dosedependently) vs. sham and control groups (p < 0.05). RT-PCR results showed that CatSper 2, 3, 4 genes expressions were reduced with 35 and 70 mg/kg injected Kerack when compared with control testes (p ≤ 0.05). However, CatSper1 expression was only reduced with high dose injected Kerack (70 mg/kg) in comparison to control testes (p ≤ 0.05). This study shows the deleterious effects of Kerack used in Iran on testis structure and sperm parameters in general, and particularly sperm morphology in adult mouse. It could down-regulate the expression of CatSper genes, resulting in depression of sperm motility.

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A Quantitative and Qualitative Study of Rat Testis Following Administration of Methadone and Buprenorphine

Cited by 22 publications

References 22 publications

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Histopathological and Biochemical Effects of Acute & Chronic Tramadol drug Toxicity on Liver, Kidney and Testicular Function in Adult Male Albino Rats

Correlation between expression of CatSper family and sperm profiles in the adult mouse testis following Iranian Kerack abuse

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