A Qualitative and Quantitative Analysis of AgNORs in Keratocystic Odontogenic Tumor, Unicystic Ameloblastoma and Multicystic Ameloblastoma

Ananthaneni, Anuradha; Udayashankar, Urmila; Guduru, Vijay Srinivasvas; Ramprasad, V. V. S.; Ramisetty, Sabitha Devi; Namala, Srilekha; Badavath, Kiran Kumar Naik

doi:10.7860/jcdr/2014/8602.4909

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…Our study showed highest index of AgNORs and Ki-67 in OKC compared to DC and UA. Similar findings were reported in previous studies (Ananthaneni et al, 2014;A. Gadbail et al, 2012;A.…”

Section: Discussionsupporting

confidence: 93%

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Evaluation of cell proliferation in cystic lesions associated with impacted third molars

Embaló

Parize

Rivero

2018

Microscopy Res & Technique

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The purpose of this study was to evaluate the metabolism and epithelial cell proliferation of odontogenic keratocyst (OKC), dentigerous cyst (DC), and unicystic ameloblastoma (UA) by quantifying the nucleolar organizing regions (AgNORs) and Ki-67 protein immunoexpression. Forty-eight cases (16 OKC, 16 DC, and 16 UA) were evaluated retrospectively. The metabolism and epithelial cell proliferation was measured by the Ki-67 positive cell percentage index and by the mean AgNOR count in each group. The Ki-67 and AgNOR counts were significantly higher in OKC comparing to the DC and UA (p < .001). Ki-67 positive cells were observed higher in suprabasal cell layers of OKC with uniform distribution, a few of them were predominantly observed in basal cell layer in DC and UA. The AgNOR count was significantly higher in the OKC basal cell layers and observed throughout the lining epithelium of DC and UA. Ki-67 and AgNOR reinforced the aggressive character of OKC, presenting high metabolism and cellular proliferation compared to DC and UA, possibly due to its more aggressive clinical behavior and high recurrence rate.Research Highlights 1. We evidence higher metabolism and epithelial cell proliferation in OKC when compared to UA and DC, supporting its aggressive aspect and its high rate of recurrence. OKC had intense and predominant labeling ofKi-67 on the suprabasal layer unlike UA and DC. K E Y W O R D S cell proliferation, metabolism, odontogenic cyst, impacted tooth, odontogenic tumors

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“…Our study showed highest index of AgNORs and Ki-67 in OKC compared to DC and UA. Similar findings were reported in previous studies (Ananthaneni et al, 2014;A. Gadbail et al, 2012;A.…”

Section: Discussionsupporting

confidence: 93%

“…DC is less aggressive and deserves special attention because of its possible transformation into ameloblastoma (Bhushan, Rao, Navata, & Kumar, 2014). The AgNOR's and Ki-67 counting have been useful for evaluating the aggressive behavior of OKC, DC, and UA (Ananthaneni, Udayashankar, Guduru, & Ramprasad, 2014;Jaafari-ashkavandi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cell proliferation in cystic lesions associated with impacted third molars

Embaló

Parize

Rivero

2018

Microscopy Res & Technique

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“…The qualitative analysis showed small to large oval AgNOR's in keratocystic odontogenic tumor and few clusters in multicystic ameloblastoma whereas in unicystic ameloblastoma irregular clusters were seen and the results are correlating with present study. 12 Navjeevraj et al, (2016) and Alessandra Dutra da Silva et al, (2016) assessed and compared the role of AgNORs in cell proliferation between ameloblastic carcinoma, solid and cystic variants of ameloblastoma. They found higher mean AgNOR in ameloblastic carcinoma than in solid ameloblastoma and unicystic ameloblastoma.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Argyrophilic Nucleolar Organizer Regions in Variants of Ameloblastoma

Suvarna¹,

Anusai²,

Someshwar³

et al. 2018

IJCMR

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Introduction: Ameloblastomas are locally invasive, highly destructive benign tumors of odontogenic origin. There are various methods performed for evaluation of proliferative activity among which quantification of argyrophilic nucleolar organizer region is a simple method. Aim of the present study is to compare number of argyrophilic nucleolar organizer region in unicystic and multicystic variants of ameloblastoma in order to analyze their cell proliferation rates. Material and Methods: This retrospective study was carried out on 10 cases each of unicystic and multicystic variants of ameloblastoma. To identify the nucleolar organizer regions, the sections were obtained and stained with silver staining technique. Argyrophilic nucleolar organizer regions were quantified by manual counting method using the image analyzer software, ProgRes CapturePro v2.8.8. Results: The Argyrophilic nucleolar organizer region count showed a significant higher number of argyrophilic nucleolar organizer regions in multicystic ameloblastoma (mean=4.236) than in unicystic ameloblastoma (mean=2.684). Conclusion: This study shows the expediency of argyrophilic nucleolar organizer region staining in reflecting the high proliferation rate and a more aggressive behavior of multicystic ameloblastoma in comparison to unicystic ameloblastoma.

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“…[ 22 ] The impaired nuclear activity in proliferating cells results in a higher AgNOR count, which relates to the lesion's malignant potential. [ 23 ] Quantification of interphase AgNORs is useful in the assessment of cell kinetics. [ 24 ] Determination of ploidy, proliferation activity, and metabolic cell activity not associated with proliferation by AgNOR count has been described as a good method.…”

Section: Discussionmentioning

confidence: 99%

Prognostic potential of AgNORs in oral submucous fibrosis

Murgod

Channabasaviah

Shivamurthy

et al. 2016

J Int Soc Prevent Communit Dent

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Aim and Objective:The role of prognosis cannot be stressed enough, especially when it comes to potentially malignant lesions. The argyrophilic nucleolar organizer regions (AgNORs), which is simple and cost-effective has been used in diagnostic and prognostic pathologies. This study seeks to identify the nucleolar organizer regions (NORs) in oral submucous fibrosis (OSMF), to correlate the AgNOR count with the histologic grade of OSMF, and to evaluate the prognostic potential of AgNOR.Materials and Methods:The sample size consisted of archival paraffin blocks of 35 cases of varying grades of OSMF and 10 cases of squamous cell carcinoma. Normal mucosa samples served as controls for the study. AgNOR staining in accordance with the method of Smith and Crocker was performed and Student's t-test was used for statistical analysis.Results:The results showed an increase in AgNOR counts with corresponding grades of OSMF, the count being least in normal mucosa and also an increase in AgNOR count with corresponding decrease in differentiation of oral squamous cell carcinoma.Conclusion:AgNOR staining is a rapid and inexpensive procedure representing cellular proliferation that can be used to assess the nature of the lesion and therefore, the prognosis.

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A Qualitative and Quantitative Analysis of AgNORs in Keratocystic Odontogenic Tumor, Unicystic Ameloblastoma and Multicystic Ameloblastoma

Abstract: This concludes the expediency of AgNOR staining in reflecting the high proliferation rate and a more aggressive behavior of KCOT in comparison to MA and UA which signifies requirement of a more hostile surgical approach in KCOT to avoid recurrences following different treatment modalities.

Cited by 4 publications

References 7 publications

Evaluation of cell proliferation in cystic lesions associated with impacted third molars

Evaluation of cell proliferation in cystic lesions associated with impacted third molars

Analysis of Argyrophilic Nucleolar Organizer Regions in Variants of Ameloblastoma

Prognostic potential of AgNORs in oral submucous fibrosis

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