A Pyrazolate Osmium(VI) Nitride Exhibits Anticancer Activity through Modulating Protein Homeostasis in HepG2 Cells

Abstract

Interest in the third-row transition metal osmium and its compounds as potential anticancer agents has grown in recent years. Here, we synthesized the osmium(VI) nitrido complex Na[OsVI(N)(tpm)2] (tpm = [5-(Thien-2-yl)-1H-pyrazol-3-yl]methanol), which exhibited a greater inhibitory effect on the cell viabilities of the cervical, ovarian, and breast cancer cell lines compared with cisplatin. Proteomics analysis revealed that Na[OsVI(N)(tpm)2] modulates the expression of protein-transportation-associated, DNA-metabolism-associated, and oxidative-stress-associated proteins in HepG2 cells. Perturbation of protein expression activity by the complex in cancer cells affects the functions of the mitochondria, resulting in high levels of cellular oxidative stress and low rates of cell survival. Moreover, it caused G2/M phase cell cycle arrest and caspase-mediated apoptosis of HepG2 cells. This study reveals a new high-valent osmium complex as an anticancer agent candidate modulating protein homeostasis.

C24H20N2O7, A NOVEL ISONICOTINAMIDE DERIVATIVE, REGULATES THE PROLIFERATION AND APOPTOSIS OF HepG2 CELLS THROUGH REGULATION OF AUTOPHAGY, ROS PRODUCTION AND THE ER

“…SOD2 promotes the effects of ROS, which in turn affects a cell's lifespan. Other study have shown that a Pyrazolate Osmium(VI) Nitride induces apoptosis through promoting HepG2 cells to release ROS [22]. These proteins are also the chemotherapeutic targets of cephalosporin antibiotics against cancers [23].…”