A putative transglycosylase encoded by &amp;lt;italic&amp;gt;SCO4132&amp;lt;/italic&amp;gt; influences morphological differentiation and actinorhodin production in &amp;lt;italic&amp;gt;Streptomyces coelicolor&amp;lt;/italic&amp;gt;

Xie, Pengfei; Zeng, Ana; Lv, Xiaoting; Cheng, Qiuxiang; Qin, Zhongjun

doi:10.1093/abbs/gmt012

Cited by 2 publications

(2 citation statements)

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“…Many previously reported genes involved in stress response, signal transduction, and transcriptional regulation were found in our study to modulate ACT production, including genes involved in cAMP signaling ( SCO4928 and SCO3571 ) (57, 58), oxidative stress ( SCO2987 ) (59), osmotic stress ( SCO5748 ) (53), and transcriptional regulation ( wblA , sarA , atrA , afsR , rsfA , SCO3269 , and SCO3723 ) (21, 24, 41, 43, 60–63). Some of these proteins regulate antibiotic production by changing the expression of the biosynthetic gene clusters, e.g., atrA , nsdA , SCO2179 , and wblA (41, 43, 64, 65), or activation of the substrate, e.g., SCO1596 (66).…”

Section: Discussionmentioning

confidence: 52%

“…1D). These peaks included the previously reported pleiotropic antibiotic negative-regulator gene wblA ( SCO3579 ) (41), the putative ATP/GTP-binding membrane protein gene SCO5677 (42), a cluster of membrane protein genes, cmdBCDEF (25), the putative secreted lytic transglycosylase gene tgdA (43), and the cytochrome bd oxidase and transporter genes cydABCD . In total, 50 genes had more than one insertion associated with increased ACT production, and 34 of these genes were not previously known to influence ACT production (Table S1).…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in Streptomyces coelicolor

Wang

et al. 2019

Appl Environ Microbiol

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Streptomyces species are important antibiotic-producing organisms that tightly regulate their antibiotic production. Actinorhodin is a typical antibiotic produced by the model actinomycete Streptomyces coelicolor. To discover the regulators of actinorhodin production, we constructed a library of 50,000 independent mutants with hyperactive Tn5 transposase-based transposition systems. Five hundred fifty-one genes were found to influence actinorhodin production in 988 individual mutants. Genetic complementation suggested that most of the insertions (76%) were responsible for the changes in antibiotic production. Genes involved in diverse cellular processes such as amino acid biosynthesis, carbohydrate metabolism, cell wall homeostasis, and DNA metabolism affected actinorhodin production. Genome-wide mutagenesis can identify novel genes and pathways that impact antibiotic levels, potentially aiding in engineering strains to optimize the production of antibiotics in Streptomyces. IMPORTANCE Previous studies have shown that various genes can influence antibiotic production in Streptomyces and that intercommunication between regulators can complicate antibiotic production. Therefore, to gain a better understanding of antibiotic regulation, a genome-wide perspective on genes that influence antibiotic production was needed. We searched for genes that affected production of the antibiotic actinorhodin using a genome-wide gene disruption system. We identified 551 genes that altered actinorhodin levels, and more than half of these genes were newly identified effectors. Some of these genes may be candidates for engineering Streptomyces strains to improve antibiotic production levels.

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