Synthesis of the spore envelope in the developmental life cycle of Streptomyces coelicolor

Sigle, Steffen; Ladwig, Nils; Wohlleben, Wolfgang; Muth, Günther

doi:10.1016/j.ijmm.2014.12.014

Cited by 17 publications

(15 citation statements)

References 51 publications

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“…Additionally, the Streptomyces spore wall synthesizing complex (SSSC) does not only direct synthesis of the peptidoglycan layer but is also involved in the incorporation of anionic spore wall glycopolymers, which contribute to the resistance of spores. The SSSC also contains eukaryotic type serine/threonine kinases which might control its activity by protein phosphorylation [57]. Genetic analysis of differentiation in Streptomyces coelicolor has identified two classes of regulatory mutants, blocked in distinct stages of differentiation.…”

Section: The Molecular Mechanisms Of Morphological Differentiationmentioning

confidence: 99%

Morphological Identification of Actinobacteria

Li¹,

Chen²,

Jiang³

et al. 2016

Actinobacteria - Basics and Biotechnological Applications

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Actinobacteria is a phylum of gram-positive bacteria with high G+C content. Among gram-positive bacteria, actinobacteria exhibit the richest morphological differentiation, which is based on a filamentous degree of organization like filamentous fungi. The actinobacteria morphological characteristics are basic foundation and information of phylogenetic systematics. Classic actinomycetes have well-developed radial mycelium, which can be divided into substrate mycelium and aerial mycelium according to morphology and function. Some actinobacteria can form complicated structures, such as spore, spore chain, sporangia, and sporangiospore. The structure of hyphae and ultrastructure of spore or sporangia can be observed with microscopy. Actinobacteria have different cultural characteristics in various kinds of culture media, which are important in the classification identification, general with spores, aerial hyphae, with or without color and the soluble pigment, different growth condition on various media as the main characteristics. The morphological differentiation of actinobacteria, especially streptomycetes, is controlled by relevant genes. Both morphogenesis and antibiotic production in the streptomycetes are initiated in response to starvation, and these events are coupled.

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Section: The Molecular Mechanisms Of Morphological Differentiationmentioning

confidence: 99%

Morphological Identification of Actinobacteria

Li¹,

Chen²,

Jiang³

et al. 2016

Actinobacteria - Basics and Biotechnological Applications

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show abstract

“…Its presence is related with fecal contamination, together with enterococci and it was chosen as model to test the sterilization of scaffold for tissue engineering applications [36]. In order to investigate the spore sterilization, S. coelicolor was selected because it has an optimal growth temperature close to the human physiological interval so, in principle, it can proliferate in scaffolds used for biomedical applications [37]. Moreover, these spores can be easily manipulated in laboratory and their growth becomes visible in culture plate after 48-72 h.…”

Section: Preparation Of Bacteria Suspensionsmentioning

confidence: 99%

Sterilization of macroscopic poly(l-lactic acid) porous scaffolds with dense carbon dioxide: Investigation of the spatial penetration of the treatment and of its effect on the properties of the matrix

Lanzalaco

Campora

Brucato

et al. 2016

The Journal of Supercritical Fluids

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“…Since the protein-protein interaction pattern of the S . coelicolor Mre proteins highly resembled the interaction pattern of Mre proteins forming the lateral wall synthesizing complex of rod-shaped bacteria, the term Streptomyces spore wall synthesizing complex SSSC was coined [ 28 , 29 ]. Screening of a genomic library for additional components of the SSSC identified SCO4778 (PkaI), encoding a eukaryotic type serine/threonine kinase (eSTPK) as an interaction partner of MreC, MreD, PBP2, and Sfr [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Control of Morphological Differentiation of Streptomyces coelicolor A3(2) by Phosphorylation of MreC and PBP2

et al. 2015

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During morphological differentiation of Streptomyces coelicolor A3(2), the sporogenic aerial hyphae are transformed into a chain of more than fifty spores in a highly coordinated manner. Synthesis of the thickened spore envelope is directed by the Streptomyces spore wall synthesizing complex SSSC which resembles the elongasome of rod-shaped bacteria. The SSSC includes the eukaryotic type serine/threonine protein kinase (eSTPK) PkaI, encoded within a cluster of five independently transcribed eSTPK genes (SCO4775-4779). To understand the role of PkaI in spore wall synthesis, we screened a S. coelicolor genomic library for PkaI interaction partners by bacterial two-hybrid analyses and identified several proteins with a documented role in sporulation. We inactivated pkaI and deleted the complete SCO4775-4779 cluster. Deletion of pkaI alone delayed sporulation and produced some aberrant spores. The five-fold mutant NLΔ4775-4779 had a more severe defect and produced 18% aberrant spores affected in the integrity of the spore envelope. Moreover, overbalancing phosphorylation activity by expressing a second copy of any of these kinases caused a similar defect. Following co-expression of pkaI with either mreC or pbp2 in E. coli, phosphorylation of MreC and PBP2 was demonstrated and multiple phosphosites were identified by LC-MS/MS. Our data suggest that elaborate protein phosphorylation controls activity of the SSSC to ensure proper sporulation by suppressing premature cross-wall synthesis.

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Synthesis of the spore envelope in the developmental life cycle of Streptomyces coelicolor

Cited by 17 publications

References 51 publications

Morphological Identification of Actinobacteria

Morphological Identification of Actinobacteria

Sterilization of macroscopic poly(l-lactic acid) porous scaffolds with dense carbon dioxide: Investigation of the spatial penetration of the treatment and of its effect on the properties of the matrix

Control of Morphological Differentiation of Streptomyces coelicolor A3(2) by Phosphorylation of MreC and PBP2

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