A purified membrane protein from Akkermansia muciniphila blunted the sepsis-induced acute lung injury by modulation of gut microbiota in rats

Han, Bing; Ke, Chao; Wang, Dong; Sun, Yali; Ding, Xianfei; Zhang, Xiaojuan; Liu, Shaohua; Du, Juan; Luo, Yi; Wang, Haixu; Duan, Xiaoguang; Zhao, Huan; Sun, Tongwen

doi:10.1016/j.intimp.2023.110432

Cited by 5 publications

(5 citation statements)

References 59 publications

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“…51,52 As noted in the literature, A. muciniphila has been found to improve LPS-induced lung injury. 51,53 A. muciniphila induced significant improvement in both the F/B ratio and the relative abundance of A. muciniphila in our study. Moreover, this study found that Klebsiella was enriched in animals with LPS-induced ALI.…”

Section: Discussionsupporting

confidence: 59%

“…Numerous studies have shown that the microbiome in the respiratory tract contributes to a variety of lung diseases, such as idiopathic pulmonary fibrosis, asthma and lung cancer. 64 Recent studies have examined whether ALI is correlated with a change in the gut microbiota, 51,53 but few have investigated the difference in the lung microbiota in lung injury. Composition analysis of the lung microbiota showed that the abundance of Firmicutes significantly increased after treatment, while Proteobacteria abundance significantly increased after LPS exposure in our study.…”

Section: Discussionmentioning

confidence: 99%

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Akkermansia muciniphila attenuated lipopolysaccharide-induced acute lung injury by modulating the gut microbiota and SCFAs in mice

Shen,

Wang,

Xia

et al. 2023

Food Funct.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Akkermansia muciniphila attenuated lipopolysaccharide-induced acute lung injury by modulating the gut microbiota and SCFAs in mice

Shen,

Wang,

Xia

et al. 2023

Food Funct.

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“…Moreover, the LPS group showed enrichment of the harmful bacteria Parabacteroides and Citrobacter. Previous studies showed that Parabacteroides was enriched in septic lung-injured rats [54], whereas Citrobacter was significantly enriched in mice coinfected with IAV-MRSA [55]. The LR group showed a decreased abundance of the harmful bacteria Morganella [56] and Citrobacter but an increased abundance of the beneficial bacteria Akkermansia, Colidextribacter and the Eubacterium__ventriosum_group.…”

Section: Discussionmentioning

confidence: 87%

“…One study has shown that Colidextribacte reduces LPS-induced liver injury and inflammation by modulating hepatic TLR4 and NF-κB signaling [57]. Akkermansia has been suggested to ameliorate LPS-induced lung injury and inflammation in numerous studies [54,58]. Notably, the LR group, although not enriched for L. reuteri, showed an increased abundance of beneficial bacteria capable of ameliorating inflammation upon L. reuteri intervention.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice

Shen,

Wang,

Huang

et al. 2023

Nutrients

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Acute lung injury (ALI) causes lung inflammation and edema as well as resulting in gut microbiota disorder. Probiotics, however, can improve the gut microbiota composition and modulate its immune response, playing an important role in ALI pathogenesis. Therefore, our study aims to investigate the effect of Lactobacillus reuteri on Lipopolysaccharide (LPS)-induced ALI in mice and to probe the mechanism of its synergistic modulatory effect on the lungs and intestines. We assessed the therapeutic effects of L. reuteri in the ALI mouse model by histopathology, alveolar lavage fluid and serum inflammatory factor analysis and explored microbiome and transcriptome alterations. L. reuteri intervention effectively attenuated lung tissue injury and significantly reduced the LPS-induced inflammatory response and macrophage and neutrophil infiltration. Additionally, L. reuteri improved the intestinal barrier function and remodeled the disordered microbiota. In conclusion, our study showed that L. reuteri attenuated the inflammatory response, ameliorated the pulmonary edema, repaired the intestinal barrier, and remodeled the gut microbiota in ALI mice. This study provides new perspectives on the clinical treatment of ALI.

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“…30,31 Moreover, its abundance increased in the sepsis group after treatment with related drugs. 30,32 The Bacteroidota, also known as Bacteroidetes, is an anaerobic bacterium that plays a role in immunity and 33 It is usually associated with gut inflammation, and its abundance is also different before and after the treatment of sepsis. 34,35 In the Circos diagram, Firmicutes and Bacteroidota is a dominant species at the phylum level.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

Zuo,

Pei,

Liu

et al. 2023

IDR

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Background Sepsis is a life-threatening organ dysfunction caused by the host’s dysfunctional response to infection, which can cause acute gastrointestinal injury (AGI). The gut microbiota is dynamic and plays a role in the immune and metabolic. The aim of this study was to investigate the composition and function of gut microbiota in patients with sepsis, as well as the gut microbiome that may be involved in the occurrence of AGI. Methods A total of 23 stool samples from healthy control individuals and 41 stool samples from sepsis patients were collected. Patients with sepsis were followed up for one week to observe whether AGI has occurred. Finally, 41 patients included 21 sepsis complicated with AGI (referred to as Com-AGI) and 20 sepsis without complicated with AGI (referred to as No-AGI). The gut microbiota was analyzed by 16S rRNA gene sequencing, followed by composition analysis, difference analysis, correlation analysis, functional prediction analysis. Results The diversity and evenness of gut microbiota were decreased in patients with sepsis. Compared with No-AGI, the gut microbiota of Com-AGI has higher community diversity, richness, and phylogenetic diversity. Escherichia-Shigella, Blautia and Enterococcus may be important indicators of sepsis. The correlation analysis showed that aspartate aminotransferase (AST) and Barnesiella have the most significant positive correlation. Moreover, Clostridium_innocuum_group, Christensenellaceae_R-7_group and Eubacterium were all significantly correlated with LAC and DAO. Clostridium_innocuum_group, Barnesiella, Christensenellaceae_R-7_group and Eubacterium may play important roles in the occurrence of AGI in sepsis. PICRUSt analysis revealed multiple functional pathways involved in the relationship between gut microbiota and sepsis, including starch degradation V, glycogen degradation I (bacterial), Lipoic acid metabolism and Valine, leucine and isoleucine biosynthesis. BugBase analysis showed that the gut microbiota with Aerobic phenotype may play an important role in sepsis. Conclusion Dysfunction of gut microbiota was associated with sepsis and AGI in patients with sepsis.

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A purified membrane protein from Akkermansia muciniphila blunted the sepsis-induced acute lung injury by modulation of gut microbiota in rats

Cited by 5 publications

References 59 publications

Akkermansia muciniphila attenuated lipopolysaccharide-induced acute lung injury by modulating the gut microbiota and SCFAs in mice

Akkermansia muciniphila attenuated lipopolysaccharide-induced acute lung injury by modulating the gut microbiota and SCFAs in mice

Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice

Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

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