A proteomic study using zebra mussels (D. polymorpha) exposed to benzo(α)pyrene: The role of gender and exposure concentrations

Does zebra mussel (Dreissena polymorpha) represent the freshwater counterpart of Mytilus in ecotoxicological studies? A critical review

“…Results indicated that dl-PCBs toxicity was not dependent on AhR binding whereas involved mostly proteins related to glycolysis pathway, cytoskeletal functions, cellular metabolism and stress response. Gender-related differences in the protein expression profiles were also underlined, confirming data from the previous paper of Riva et al (2011).…”

“…Nevertheless, it is absolutely needed an in-depth approach with the help of the state-of-the-art technologies available especially in the field of the Riva and Binelli, 2014 protein identification when we used a non-sequenced organism. In fact, Riva et al (2011) identified only 37% of the protein changed by B[a]P because of they used the peptide mass fingerprint (PMF) for the protein identification, which is very useful only when the genome of the biological model is fully sequenced. By contrast, in the further studies, the recourse to de novo sequencing approach allowed the identification for homology of a higher percentage of protein varied, ranging from 62% (Riva and Binelli, 2014) to even the 100% of identification .…”

“…However, although this drawback, several proteomic studies are performed on zebra mussel to identify biochemical pathways involved in the toxicity mechanism of environmental pollutants (Table 5). The first study in which D. polymorpha was used as a suitable biological model for proteomics has been shown in the paper of Riva et al (2011). The authors evaluated the role of gender and exposure concentrations by exposing zebra mussel to waterborne B[a]P environmental concentrations for 7 days.…”

Toxicological effects of benzo(a)pyrene, DDT and their mixture on the green mussel Perna viridis revealed by proteomic and metabolomic approaches

“…SOD is a typical antioxidant enzyme and plays a critical role in ROS (reactive oxygen species) scavenging (Hern andez-Nistal et al, 2002;Kim et al, 2011). In mussel, oxidative stress is a prominent feature of DDT toxicity, seen from the increase of gelsolin and actin, it ultimately generate complex cytoskeletal rearrangements in gill cell (Riva et al, 2011;Thrower et al, 2010).…”

PROTEOMICS in aquaculture: Applications and trends

“…Many authors have been trying to establish protein patterns of environmental contaminants exposure, as well as comparing protein profiles of bivalves residing in polluted areas to those of reference sites [65][66][67][68]. Environmental contaminants proteomics studies include exposure to cadmium [69] NP (nonylphenol) [70] and p,p-dichlorodiphenyldichloroethylene (DDE) in the clam Ruditapes decussates, benzo(α)pyrene in zebra mussels (D. polymorpha) [71] PCBs, PAHs (polyaromatic hydrocarbons) and heavy metals in blue mussels (Mytilus edulis) [72,73], cylindrospermopsin (CYN) in Mytilus galloprovincialis and Corbicula fluminea [74] and crude oil in blue mussels [75][76][77].…”