Toxicological effects of benzo(a)pyrene, DDT and their mixture on the green mussel Perna viridis revealed by proteomic and metabolomic approaches

Song, Qinqin; Chen, Hao; Li, Yuhu; Zhou, Hailong; Han, Qian; Diao, Xiaoping

doi:10.1016/j.chemosphere.2015.08.029

Cited by 52 publications

(37 citation statements)

References 95 publications

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“…The -omics techniques (genomics, transcriptomics, proteomics, and metabolomics) can compare the whole patterns of molecules (genes, proteins, and metabolite) to present the differences between normal and stressed biological samples, and therefore, they have been extensively used to elucidate the biological effects induced by environmental stressors in organisms (Ji et al, 2013;Santos et al, 2010;Song et al, 2016aSong et al, , 2016b. Among these -omics techniques, metabolomics focuses on the whole set of metabolites (b 1000 Da) in organisms (Jones et al, 2008;Viant et al, 2003).…”

Section: H Nmrmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oMarine environment in the Laizhou Bay is potentially contaminated by metals from industrial discharges. In this study, metal concentrations in shrimps Crangon affinis indicated that two typical sites (S6283 and S5283) close to Longkou and Zhaoyuan cities along the Laizhou Bay have been contaminated by metals, including Cd, As, Cu, Ni, Co, and Mn. In particular, Cd and As were the main metal contaminants in S6283. In S5283, however, Cu was the most important metal contaminant. The metabolic responses in the shrimps indicated that the metal pollution in S6283 and S5283 induced disturbances in osmotic regulation and energy metabolism and reduced anaerobiosis, lipid metabolism, and muscle movement. However, alteration in the levels of dimethylglycine, dimethylamine, arginine, betaine, and glutamine indicated that the metal pollution in S5283 induced osmotic stress through different pathways compared to that in S6283. In addition, dimethylamine might be the biomarker of Cu in shrimp C. affinis.

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Section: H Nmrmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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“…Since proteomics and metabolomics can directly characterize the minor metabolite and protein responses, these two eomic techniques and their combination have represented their practicability to elucidate the biological effects and mechanisms of pollutants in environmental bioindicators. Especially, a combination of proteomics and metabolomics could validate and complement one another, when testing the biological effects of environmental pollutants [11,12].…”

Section: Introductionmentioning

confidence: 99%

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

et al. 2016

Fish & Shellfish Immunology

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a b s t r a c tCadmium (Cd) and arsenic (As) are the main metal/metalloid contaminants in the coastal environments of the Bohai Sea, China. In this work, a combined proteomic and metabolomic approach was applied to investigate the biological effects of Cd and As (V) in the early life stage (D-shape larvae) of mussel Mytilus galloprovincialis. Results indicated that Cd was a potential immune toxicant to D-shape larval mussel because of the numerous proteomic responses related to immune system. Additionally, Cd induced oxidative stress, cellular injury and disturbance in nucleic acid metabolism in D-shape larval mussels. However, only two identified proteins were significantly altered in As (V)-treated group, suggesting that D-shape larval mussel was less sensitive to As (V) than to Cd at protein level. These two proteins in response to As (V) suggested that As (V) influenced anti-oxidative system and cell proliferation in Dshape larval mussels. Metabolic responses indicated that Cd and As (V) induced disturbances in osmotic regulation and energy metabolism in D-shape larval mussels via different metabolic pathways. In addition, Cd reduced lipid metabolism as well. This work demonstrated that a combination of proteomics and metabolomics could provide an insightful view in the biological effects of pollutants in mussel M. galloprovincialis at an early life stage.

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“…; Song et al . ); characterizing the consequence of pesticide exposure in carp (Kokushi et al . ); assessing the effects of petrochemical contamination at industrialized sites harbouring caged mussels (Fasulo et al .…”

Section: Metabolomics Applications In Aquaculturementioning

confidence: 99%

“…Using fish and shellfish, metabolomics is progressively being used to characterize mechanisms of toxicity and to develop novel methods to assess environmental contamination. For example, metabolomics-based studies have proven useful for: identifying physiological responses of various molluscs to agricultural runoff, heavy metals and endocrine disruptors Hanana et al 2014;Ji et al 2014Ji et al , 2015aJi et al ,b, 2016Leonard et al 2014;Zhou et al 2014;Campillo et al 2015;Song et al 2016); characterizing the consequence of pesticide exposure in carp (Kokushi et al 2015); assessing the effects of petrochemical contamination at industrialized sites harbouring caged mussels (Fasulo et al 2012;Cappello et al 2013Cappello et al , 2015; detecting freshwater locations which have multicontaminant sediment loadings (Watanabe et al 2015); identifying new and highly sensitive bioindicator species of clams for monitoring environmental contamination (Ji et al 2015c); and developing noninvasive methods for pollution assessment by profiling metabolites in the excreted mucus of fish skin (Ekman et al 2015). The results of these studies clearly demonstrate promising applications.…”

Section: Disease and Immunologymentioning

confidence: 99%

Showcasing metabolomic applications in aquaculture: a review

Young

Alfaro

2016

Reviews in Aquaculture

115

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Aquaculture production is currently challenged to meet the growing demands for seafood protein throughout the world. To achieve this growth in an efficient, safe and sustainable manner, novel tools and applications will need to be incorporated at each step of the production line. A variety of 'omics' (e.g., transcriptomics, proteomics, metabolomics) applications have already begun to emerge in aquaculture research with extreme success. A promising new 'omics' approach is metabolomics, which aims to use metabolite profiles to identify biomarkers indicative of physiological responses of living samples (e.g., whole organism, tissues, cells) to environmental or culture conditions. One of the benefits of this approach is that it uses a broad scan of biological conditions to identify often unexpected problem or risk areas to focus management attention. In this contribution, we have selected relevant research examples to showcase the applications of metabolomics in aquaculture in four major areas: hatchery production, nutrition and diet, disease and immunology, and food safety and quality. The novelty of this approach is highlighted by the fact that we have cited the majority of published papers in this field, and these are all recent (within the last decade) contributions.

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Toxicological effects of benzo(a)pyrene, DDT and their mixture on the green mussel Perna viridis revealed by proteomic and metabolomic approaches

Cited by 52 publications

References 95 publications

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

Showcasing metabolomic applications in aquaculture: a review

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