A proteomic approach to identify differentially expressed plasma proteins between the fed and prolonged fasted states

Bertile, Fabrice; Schaeffer, Christine; Maho, Yvon Le; Raclot, Thierry; Dorsselaer, Alain Van

doi:10.1002/pmic.200701001

Cited by 25 publications

(30 citation statements)

References 55 publications

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“…3; Tables S1 and S2), of which five had not been reported in phase 3 fasting rats18. Among these ten downregulated proteins, most are known to be involved in the regulation of energy balance19; they also play a role in insulin resistance and lipid metabolism, immunity and inflammation, and bone metabolism2021; they are furthermore linked with cardiovascular risks and haemostasis, liver failure, and renal pathologies2122.…”

Section: Resultsmentioning

confidence: 99%

The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism

Bertile

Fouillen

Wasselin

et al. 2016

Sci Rep

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While safety of fasting therapy is debated in humans, extended fasting occurs routinely and safely in wild animals. To do so, food deprived animals like breeding penguins anticipate the critical limit of fasting by resuming feeding. To date, however, no molecular indices of the physiological state that links spontaneous refeeding behaviour with fasting limits had been identified. Blood proteomics and physiological data reveal here that fasting-induced body protein depletion is not unsafe “per se”. Indeed, incubating penguins only abandon their chick/egg to refeed when this state is associated with metabolic defects in glucose homeostasis/fatty acid utilization, insulin production and action, and possible renal dysfunctions. Our data illustrate how the field investigation of “exotic” models can be a unique source of information, with possible biomedical interest.

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Section: Resultsmentioning

confidence: 99%

The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism

Bertile

Fouillen

Wasselin

et al. 2016

Sci Rep

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“…In agreement with this study, proteomic analysis showed decreased plasma DBP and reduced liver DBP mRNA concentrations during prolonged fasting. 23 Independent of the precipitation method we used, a partial co-precipitation of DBP, VLDL plus LDL particles was observed. This finding is in line with the results obtained by HPGPC and ultracentrifugation.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the potential association of vitamin D binding protein with lipoproteins

et al. 2010

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Background: Vitamin D binding protein (DBP) acts as a vitamin D carrier and an actin scavenger. We have previously reported a correlation between serum DBP and lipid parameters in a cystic fibrosis population. In the present study, the relationship between serum DBP, lipoprotein fractions and vitamin D is investigated. Methods: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)-and 1.25(OH) 2 -vitamin D 3 were assayed by radioimmunoassay. Total cholesterol, high-density lipoprotein cholesterol (HDL), triglycerides and insulin were measured using routine methods. Low-density lipoprotein-cholesterol (LDL) was calculated according to Friedewald's formula. Results: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D 3 in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D 3 /DBP ratio correlated negatively with serum triglyceride concentration and body mass index (BMI). The actin-bound DBP complex was identified with Western blot. Conclusions: The lipid-bound DBP fraction may be of greater importance than initially thought. The present findings may have clinical consequences in view of the important physiological role of DBP.

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“…During PIII, energy expenditure increases again (Le Maho et al, 1981;Cherel et al, 1994b) along with glucocorticoid (GC) levels and non-reproductive behaviour (Kitaysky et al, 1999;Robin et al, 2001;Groscolas et al, 2008). This gradual reallocation of energy towards soma preservation rather than current reproduction results from a complex network of metabolic and (neuro-) hormonal changes called the 're-feeding signal' (Bertile et al, 2009;Groscolas and Robin, 2001;Minokoshi et al, 2004;Groscolas et al, 2008;Spée et al, 2010), and is expected to lead to the restoration of adult body condition at the expense of reproductive success.…”

Section: Introductionmentioning

confidence: 99%

The oxidative debt of fasting: evidence for short to medium-term costs of advanced fasting in adult king penguins

Schull

Viblanc

Stier

et al. 2016

Journal of Experimental Biology

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In response to prolonged periods of fasting, animals have evolved metabolic adaptations helping to mobilize body reserves and/or reduce metabolic rate to ensure a longer usage of reserves. However, those metabolic changes can be associated with higher exposure to oxidative stress, raising the question of how species that naturally fast during their life cycle avoid an accumulation of oxidative damage over time. King penguins repeatedly cope with fasting periods of up to several weeks. Here, we investigated how adult male penguins deal with oxidative stress after an experimentally induced moderate fasting period (PII) or an advanced fasting period (PIII). After fasting in captivity, birds were released to forage at sea. We measured plasmatic oxidative stress on the same individuals at the start and end of the fasting period and when they returned from foraging at sea. We found an increase in activity of the antioxidant enzyme superoxide dismutase along with fasting. However, PIII individuals showed higher oxidative damage at the end of the fast compared with PII individuals. When they returned from re-feeding at sea, all birds had recovered their initial body mass and exhibited low levels of oxidative damage. Notably, levels of oxidative damage after the foraging trip were correlated to the rate of mass gain at sea in PIII individuals but not in PII individuals. Altogether, our results suggest that fasting induces a transitory exposure to oxidative stress and that effort to recover in body mass after an advanced fasting period may be a neglected carryover cost of fasting.

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A proteomic approach to identify differentially expressed plasma proteins between the fed and prolonged fasted states

Cited by 25 publications

References 55 publications

The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism

The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism

Investigation of the potential association of vitamin D binding protein with lipoproteins

The oxidative debt of fasting: evidence for short to medium-term costs of advanced fasting in adult king penguins

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