A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers

Sifer, C.; Sellami, Afifa; Poncelet, C.; Kulski, P.; Martin-Pont, B.; Bottéro, J.; Porcher, Raphaël; Cédrin‐Durnerin, Isabelle; Hugues, Jean‐Noël; Wolf, Jean-Philippe

doi:10.1093/humrep/del149

Cited by 53 publications

(40 citation statements)

References 41 publications

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“…However, to the best of our knowledge, no single study has been able to demonstrate sufficient evidence favourable to AH, and the current research conclusions are not unanimous (6)(7)(8)(9)(10)(11)(12)(13). In a large meta-analysis, Martins et al showed that laser-AH (LAH) is currently one of the best, safest and most effective AH methods (14).…”

Section: Introductionmentioning

confidence: 83%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to determine whether the size of the zona pellucida (ZP) thinning area by laser-assisted hatching affected the potential development of vitrified-warmed embryos. A total of 196 vitrified-warmed cleavage-stage embryos (from 49 patients, four sister embryos per patient) were used in the study, i.e., four sister embryos from each patient were randomly assigned to four groups: A control group of embryos that were not zona-manipulated (zona intact, group A); one experimental group of embryos in which a quarter of the zona pellucida was thinned using laser-assisted ZP thinning (group B); a second experimental group of embryos in which half of ZP was thinned (group C); and a third group in which two-thirds of the ZP was thinned (group D). Subsequent blastocyst development was assessed. Microscopy was performed to study the hatching process of the embryos after zona thinning. The blastocyst formation rates were 71.43% in group A, 67.35% in group B, 65.31% in group C, and 51.02% in group D (groups B-D vs. group A, P=0.661, P=0.515, P= 0.038, respectively). The rates of complete hatching were 30.61% in group A, 38.78% in group B, 61.22% in group C, and 48.98% in group D (groups B-D vs. group A, P= 0.396, P= 0.002, P= 0.063, respectively). For a subgroup of patients, there was a significant difference in the complete hatching in all the groups for women aged <35 years (P=0.011), and there was a significant difference in the complete hatching in all the groups for secondary infertility women (P=0.022). There was no significant difference in the blastocyst formation rates in the different groups of women aged ≥35 years (P= 0.340). In addition, there was no significant difference in the complete hatching in the different groups among women aged ≥35 years (P= 0.492). The results of the present study showed that in vitrified-warmed embryo transfers at the cleavage-stage, and the two-thirds zona pellucida thinning group demonstrated a significantly decreased blastocyst formation rate compared with the control group, while the half zona pellucida thinning group demonstrated a significantly increased complete hatching rate compared with the control group, which may have a high value in clinical application.

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Section: Introductionmentioning

confidence: 83%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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“…assisted hatching, may be useful for frozen-thawed embryo transfer. However, there are only a few randomized studies focused on the benefit of assisted hatching following frozen-thawed embryo transfer that have reported controversial results with decreased [2], similar [3][4][5] or increased [6][7][8][9] implantation rates in the assisted hatching group compared with the control group. This is likely related to different assisted hatching techniques (chemical, enzyme or laser) and different methods of zona pellucida manipulation (thinning or opening), in addition to differences in the study design, patient characteristics, and selection criteria.…”

Section: Introductionmentioning

confidence: 99%

Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Hiraoka

Horiuchi

Kusuda

et al. 2009

J Assist Reprod Genet

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“…assisted hatching (AH), may be useful for frozen thawed embryo transfer. However, very recent studies focused on the benefit of AH following frozen thawed embryo transfer reported controversial results with decreased [2], similar [3,4] or increased [5,6] implantation rates in the AH group compared to the control group. Different types of AH have been developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

Fuchiwaki

Hiraoka

Horiuchi

et al. 2008

J Assist Reprod Genet

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A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers

Abstract: Within the constraints of our protocol, partial enzymatic digestion of zona pellucida by pronase was not related with any benefit of the FET outcome especially concerning the implantation ability of frozen-thawed embryos.

Cited by 53 publications

References 41 publications

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

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