A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR–universal array analysis

Fruit Germplasm Characterization: Genomics Approaches for the Valorisation of Genetic Diversity

“…The "ligation detection reaction-universal array" (LDR-UA), was adopted by , to successfully genotype a panel of 49 varieties with respect to 17 SNPs. Out of the 13 amplicons containing these SNPs, 12 were successfully amplified from oil-derived template, and the resulting profiles were fully consistent with those obtained from leaf-derived DNA (Consolandi et al, 2008). qRT-PCR continues to be extensively used for quantifying the amount of a specific sequence in food, with particular interest for GMOs (Marmiroli et al, 2009).…”

Traceability of Origin and Authenticity of Olive Oil

“…Significant amounts of DNA are present in olive oil obtained by cold pressing (Consolandi et al, 2008). However, the filtration process lowers DNA concentrations, which tend to disappear due to nuclease degradation (De la Torre et al, 2004;Muzzalupo et al, 2002).…”

“…(Consolandi et al, 2008) DNA recovery methods from olive oil have been developed by many authors (Busconi et al 2003;Doveri et al 2006;Pasqualone et al 2007;Consolandi et al 2008). Several commercial kits, providing adapted protocols, were used in different works (Martins-Lopes et al 2008;Spaniolas et al 2008a;Ayed et al 2009;Pafundo et al 2010).…”

“…Martins-Lopes et al (2008) evaluated the efficiency of RAPD, ISSR and SSR molecular markers for olive oil varietal identification and their possible use in certification purposes (Bracci et al, 2011). Consolandi et al (2008) reported the development of a semi-automated SNP genotyping assay to verify the origin and the authenticity of extra-virgin olive oils. The authors developed a Ligation Detection Reaction (LDR)/Universal Array (UA) platform by using several olive SNPs.…”

“…This has promoted a growing interest towards the application of DNA-based markers since it is independent from environmental conditions. Specific protocols for DNA isolation from olive oil have been developed (Breton et al, 2004;Busconi et al, 2003;Consolandi et al, 2008;De la Torre et al, 2004). The application of DNAbased methods requests the knowledge on nucleotide sequences of olive.…”