This study analyzed the lesson plan prepared by pre-service teachers in terms of class participation. In addition, the mismatch between the lesson plan and teaching practice of pre-service biology teachers in teaching practicum. The elements of class participation were analyzed by dividing them into 'depth of knowledge', 'higher-order thinking', 'connectedness to the world beyond the classroom', 'substantive conversation', & 'social support for student achievement'. As a result of this study, the elements of class participation did not appear well in the lesson plans in the introductory stage. In particular, in the evolving stage, many elements of class participation such as 'depth of knowledge', 'higher-order thinking', and 'Connectedness to the world beyond the classroom' were presented in the class guidance. However, in many cases, the elements of class participation suggested in the lesson plans were not properly implemented in the teaching practice. This result suggests that prre-service biology teachers need to enhance their understanding of class participation when conducting classes. In addition, in the teacher training curriculum, education on strategies to activate learners' participation in class should be provided.