A prime-boost vaccination of mice with attenuated Salmonella expressing a 30-mer peptide from the Trichinella spiralis gp43 antigen

Castillo, Aida; Vaquero-Vera, A.; Fonseca-Liñán, Rocío; Ruı́z-Pérez, Fernando; Villegas‐Sepúlveda, Nicolás; Ortega‐Pierres, Guadalupe

doi:10.1016/j.vetpar.2013.01.056

Cited by 21 publications

(8 citation statements)

References 15 publications

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“…Following challenge with 300 T. spiralis infectious larvae, the mice immunized with rTsT showed a 33.17% reduction of enteral AW at 5 dpi and a 37.80% ML reduction at 30 dpi, respectively. The worm burden reduction observed in this study is similar to those obtained with subcutaneous immunization using other individual recombinant T. spiralis protein [ 19 , 28 , 52 ], but the protective level obtained in this study is lower than those of previous reports by using oral or intranasal vaccination [ 76 , 82 ]. The immune protection obtained in this study maybe owing to the results of a combination of worm expulsion from the gut, reduction of female fecundity, or ADCC killing and destruction of NBL induced by rTsT immunization, which resulted in a reduction of intestinal adults and muscle larvae in immunized mice.…”

Section: Discussionsupporting

confidence: 74%

Vaccination of Mice with a Novel Trypsin from Trichinella spiralis Elicits the Immune Protection against Larval Challenge

et al. 2020

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Trichinella spiralis is a major foodborne parasite and has a serious threat to meat safety. Development of anti-Trichinella vaccines is prospective to eliminate Trichinella infection in food animal. The aim of this study was to assess the biological properties of a novel T. spiralis trypsin (TsT) and its elicited immune protection against larval challenge. The cDNA sequence of TsT gene was cloned and expressed. Western blotting showed rTsT was identified by infection serum and anti-TsT serum. RT-PCR results revealed that TsT gene was transcribed at diverse T. spiralis lifecycle stages. The IIFT results showed that natural TsT was principally expressed at epicuticle of 5-6 day adult worms, indicating that TsT is a worm somatic antigen and adult-stage specific surface antigen. Vaccination of mice with rTsT triggered an evident humoral immune response (high levels of serum IgG, IgG1/IgG2a, and enteral sIgA), and it also induced the systemic and enteral local cellular immune response, demonstrated by an significantly elevation of cytokines IFN-γ and IL-4. The mice vaccinated with rTsT exhibited a 33.17% reduction of enteral adult worms and a 37.80% reduction of muscle larvae after larval challenge. The results showed that TsT might be considered as a candidate target antigen for anti-T. spiralis vaccines.

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Section: Discussionsupporting

confidence: 74%

Vaccination of Mice with a Novel Trypsin from Trichinella spiralis Elicits the Immune Protection against Larval Challenge

et al. 2020

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“…Since trichinellosis is mainly due to the ingestion of infected meat, oral immunization is a more appropriate route to induce long intestinal protective immunity [ 13 , 49 ]. Previous studies showed that attenuated Salmonella is an efficient live carrier for antigen delivery to elicit permanently persistent systemic and mucosal protective responses to the intestinal parasite stages, providing a valid vaccination strategy by means of oral or intranasal routes [ 50 , 51 ]. Intestinal mucosal IgA response and secretory IgA (sIgA) cells returning to intestinal epithelium could be effectively elicited by oral vaccination of the attenuated Salmonella , the sIgA has crucial roles for expelling or sacrificing intestinal parasites [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA

Guo

et al. 2018

Vet Res

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Trichinellosis is a worldwide important food-borne zoonosis caused mainly by ingesting raw or undercooked pork infected with Trichinella spiralis larvae. The development of vaccine is needed for preventing swine from Trichinella infection to ensure pork safety. Previous studies showed that T. spiralis serine protease 1.2 (TsSP1.2) is a vaccine candidate against Trichinella infection. In this study, the complete TsSP1.2 cDNA sequences were cloned into pcDNA3.1, and the rTsSP1.2 DNA was transformed into attenuated Salmonella typhimurium strain ΔcyaSL1344. Oral vaccination of mice with Salmonella-delivered rTsSP1.2 DNA vaccine induced an obvious intestinal mucosal IgA response and a systemic Th1/Th2 immune response; the vaccinated mice showed a 33.45% reduction of intestinal adult worms and 71.84% reduction of muscle larvae after T. spiralis larval challenge. The protection might be due to the rTsSP1.2-induced production of specific anti-TsSP1.2 sIgA, IgG, IgG1/IgG2a, and secretion of IFN-γ, IL-4 and IL-10, which protected intestinal mucosa from the parasite invasion, inhibited worm development and reduced female fecundity. The results indicate that the attenuated Salmonella-delivered rTsSP1.2 DNA vaccine offers a prospective strategy for the prevention and control of animal Trichinella infection.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0582-2) contains supplementary material, which is available to authorized users.

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“…Recent studies have shown that live attenuated Salmonella is an effective vector for delivery of heterologous antigens or DNA to the immune system [ 17 18 23 37 ]. In addition, live vectors can mimic the natural process of Salmonella infection through the oral route, resulting in mucosal and systemic immune responses against the pathogen's antigen [ 7 ]. In addition, live vectors can induce immune responses to aid in resisting the Salmonella infection.…”

Section: Introductionmentioning

confidence: 99%

Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

et al. 2018

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Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccine can protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologous recombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effect of this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutination inhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation were increased significantly more (p < 0.01) in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) than with C79-13ΔcrpΔasd (pYA) but were not significantly increased compared with the chickens immunized with the LaSota live vaccine (p > 0.05). Moreover, the novel strain provides 60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study, a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd (pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.

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A prime-boost vaccination of mice with attenuated Salmonella expressing a 30-mer peptide from the Trichinella spiralis gp43 antigen

Cited by 21 publications

References 15 publications

Vaccination of Mice with a Novel Trypsin from Trichinella spiralis Elicits the Immune Protection against Larval Challenge

Vaccination of Mice with a Novel Trypsin from Trichinella spiralis Elicits the Immune Protection against Larval Challenge

Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA

Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

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