A primal incrimination of<i> Cedecea davisae</i> with post-prostatectomy urinary tract infection in Nigeria

Abstract

Cedecea species are recent members of the family Enterobacteriaceae and are rarely incriminated in human infections. A patient with protracted urinary tract infection, post-prostatectomy, was referred to Lahor Research Laboratories, Benin City, Nigeria for routine culture and susceptibility testing. Three consecutive mid-stream urine samples from him yielded pure, heavy, growths of an apparently same Gram negative, nonspore forming, non-capsulated, motile, rod-shaped bacterium, which was lipase, citrate, methyl red, KCN, ONPG, arginine and ornithine decarboxylase positive. It was oxidase, urease, H 2 S, indole, gelatinase, deoxyribonuclease and phenylalanine deaminase negative. It fermented glucose, xylose, sucrose, salicin, maltose mannitol, and lactose but was negative arabinose, inositol, dulcitol, raffinose, rhamnose and melibiose. It exhibited multiple antibiotic resistances to augmentin, gentamicin, ofloxacin, cefixime, ciprofloxacin, ceftazidime and cefuroxime but was very sensitive to imipenem and moderately susceptible to nitrofurantoin. The isolate was identified phylogenetically as Cedecea davisae. It possessed plasmid at 48.5 kb and harboured SHV, TEM, and gyrA antibiotic-resistance genes, which were either plasmid-mediated, chromosomal-mediated or both. To the best of knowledge, this is the first report on the incrimination of this organism with human infection in Nigeria. It was concluded that the paucity of reports on Cedecea species as aetiologic agents of human infections could be as a result of the difficulty in recognizing and characterizing this recently described pathogen, particularly in low resource laboratories, coupled with the fact that Laboratorians and Physicians are yet to be fully aware of the emerging clinical significance of this "relatively new" organism.

Action of Fractionated Moringa oleifera Lam Leaf Extracts on Multidrug Resistant Pseudomonas aeruginosa Strains

“…The distribution of etiologic agents of surgical wound infections showed that P. aeruginosa (62.6%) was found to be more predominant. This is in conformity with the study of Agbonlahor et al (2018) who also found that P. aeruginosa were more prevalent in surgical wound infections [38]. Thirty-four (54.8%) strains were found to be multidrug resistant Pseudomonas aeruginosa isolates.…”

“…[40] reported that bacterial isolated from surgical wound infections are mostly resistant to Ceftazidime and Augmentin. This is most likely to be due to the presence of enzymes such as Cephalosporinase and Penicillinase which can prevent the activity of Beta-lactam ring of the antibiotics [38]. Current problems linked with the use of antibiotics, increased prevalence of multiple drug resistant (MDR) strains of a number of pathogenic bacteria has revitalized the interest in plants with antimicrobial properties [41].…”

Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique

“…Amplification and sequencing were done as described by Agbonlahor et al ( 21 ), with the following modifications: Purification was done with the Applied Biosystems Incorporation (ABI) V3.1 Big dye kit according to manufacturer’s instructions. The labeled products were then cleaned with the Zymo Seq clean-up kit (USA) in accordance with manufacturer’s instructions.…”

“…The ultra-pure DNA was sequenced with ABI3500XL analyser at Functional Bioscience, Madison, USA. Sequences data generated were analysed with Geneious version 9.0.5 and phylogenetic tree were constructed using neighbour-joining method as described by Agbonlahor et al ( 21 ).…”

A Rare Case of Cedecea Davisae Bacteremia Presenting as Biliary Sepsis

“…The combination of AmpC production and porin deficiency in the cell wall contributes to its multidrug resistance [2]. It possesses plasmid carrying multidrug resistance genes, through which it can transfer its genetic materials to other susceptible bacteria in the same ecosystem [3]. To the best of our knowledge, only 12 cases of C. davisae bacteremia have been reported in the literature.…”