A Preliminary Study on the Effects of Nitrogen Supply on the Growth<i>In Vitro</i>of Nine Potato Genotypes (<i>Solanum</i>spp)

Evans, N. E.

doi:10.1093/jxb/44.4.837

Cited by 19 publications

(11 citation statements)

References 11 publications

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“…Most investigations in the literature corroborate the hypothesis that the amount of N in MS media is too high for optimal regeneration and growth (Chauvin and Salesses 1988;Evans 1993;Avila et al 1998). Conversely, in our experimental system, increasing the total N led to a proportional increase in the multiplication rate, which was not at the expense of shoot growth and quality.…”

Section: Discussionmentioning

confidence: 67%

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Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Ivanova

Staden

2009

Plant Cell Tiss Organ Cult

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The role of nitrogen (N) supplied as inorganic ammonium (NH 4 ? ) and nitrate (NO 3 -), and organic glutamine on shoot regeneration, incidence of hyperhydricity and production of good quality shoots in Aloe polyphylla explants was investigated. The omission of total N from the culture medium resulted in low proliferation and hindered shoot growth. Ammonium as the sole source of N depressed shoot regeneration and growth and escalated the frequency of hyperhydricity to ca. 50%. When NO 3 -was used as the sole N source, shoots of good quality were produced and hyperhydricity was completely eliminated. Overall, the MS N combination was superior to any single N source for proliferation and growth of shoots, suggesting a synergistic effect between NH 4 ? and NO 3 -on shoot regeneration. Moreover, the relative amounts of NH 4 ? and NO 3-also influenced the parameters tested. The highest regeneration was obtained with NH 4 ? :NO 3 -ratios (mM) of 20:40, 30:30 and 40:20. Decreasing the ratio of NH 4 ? :NO 3 -lowered the incidence of hyperhydricity. This study also demonstrates the potential of glutamine as the sole source of N, since its application resulted in the production of good quality shoots and almost no hyperhydricity.

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Section: Discussionmentioning

confidence: 67%

“…Noticeable influences of the absolute and relative amounts of NO 3 -and NH 4 ? on the growth and morphogenesis of plant tissue cultures have been reported for a number of in vitro systems (Evans 1993;Tsai and Saunders 1999;Ramage and Williams 2002;Woodward et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Ivanova

Staden

2009

Plant Cell Tiss Organ Cult

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“…Estudando três espécies diferentes de orquídea, Dijik & Eck (1995a, b) observaram comportamentos diferentes conforme a espécie estudada, sendo que a produção de biomassa diminuiu em Dactylorhiza incarnata, aumentou em D. praetermissa e se manteve estável em D. majalis, com o aumento da concentração de nitrogênio no meio (entre 0 e 12 mM). Evans (1993) estudando nove cultivares de Solanum spp.…”

Section: Resultsunclassified

Efeitos de diferentes concentrações de nitrogênio no crescimento de Aechmea blanchetiana (Baker) L.B. Sm. cultivada in vitro

Kanashiro

Ribeiro

Gonçalves

et al. 2007

Hoehnea

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-(Effects of nitrogen concentrations on the growth of Aechmea blanchetiana (Baker) L.B. Sm. cultured in vitro). This work aimed to study the influence of different nitrogen concentrations (7.5; 15; 30; 60 and 120 mM) on the growth of Aechmea blanchetiana plantlets cultivated in vitro. The results showed that 7.5 mM of nitrogen in a modified liquid culture Murashige & Skoog (MS) medium was the best concentration for increasing fresh and dry mass production.

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“…Após a determinação das variáveis consideradas, aproximadamente 1 g da massa seca de folhas por repetição foi destinada à determinação de teores de macro (N, P, K, S, do= dados originais; ns = não significativo (α>0,05); * = significativo a 5% (α ≤ 0,05); ** = significativo a 1% (α ≤ 0,01), *** = significativo a 0,1% (α ≤ 0,001) Evans (1993) (1/4, 1/2 e 1/1 KNO 3 ) no meio MS, segundo Gomes & Shepherd (2000). média da massa/plântula N (mM.L -1 ) total ns = não significativo (α>0,05); * = significativo a 5% (α ≤ 0,05); ** = significativo a 1% (α ≤ 0,01), *** = significativo a 0,1% (α ≤ 0,001)…”

Section: Análises Químicasunclassified

Nitrogênio, fósforo, potássio, cálcio e o crescimento de plântulas de Aechmea blanchetiana (Baker) L.B.Smith in Vitro.

Kanashiro¹

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A Preliminary Study on the Effects of Nitrogen Supply on the GrowthIn Vitroof Nine Potato Genotypes (Solanumspp)

Cited by 19 publications

References 11 publications

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Efeitos de diferentes concentrações de nitrogênio no crescimento de Aechmea blanchetiana (Baker) L.B. Sm. cultivada in vitro

Nitrogênio, fósforo, potássio, cálcio e o crescimento de plântulas de Aechmea blanchetiana (Baker) L.B.Smith in Vitro.

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