A preliminary study on the use of a long-acting GnRH agonist prior to ovarian stimulation for oocyte retrieval in the western lowland gorilla

Yee, Bill; Loskutoff, N.M.; Cambre, Richard C.; Calle, Paul P.; Chacón, R.; Bowsher, T.R.; Kraemer, D.C.

doi:10.1016/0093-691x(90)90782-o

Cited by 7 publications

(6 citation statements)

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“…Reproductive technologies, routinely used in the human for treatment of infertility, are currently being evaluated to assist in increasing the population of captive lowland gorillas (Yee et al, 1990;Loskutoff et al, 1991). As the average age of these captive gorillas increases, so does the incidence of reproductive failure, resulting in an insufficient replacement of the breeding population (Seal and Foose, 1988).…”

Section: Introductionmentioning

confidence: 99%

Hemizona assay for measuring zona binding in the lowland gorilla

Lanzendorf

Holmgren

Johnson

et al. 1992

Molecular Reproduction Devel

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The hemizona assay is a diagnostic test used to evaluate the binding potential of spermatozoa to zonae pellucida and has been used to predict fertilization potential in the human. In this study, frozen-thawed gorilla spermatozoa were coincubated with human hemizonae to evaluate tight binding and to assess the use of human zonae in evaluating sperm fertility. Matching hemizonae were incubated with human sperm to serve as a control. For evaluation of binding studies in a homologous system, matching halves of gorilla hemizonae were coincubated with both gorilla and human sperm. Whole, intact zonae of both human and gorilla oocytes were also coincubated with heterologous sperm to determine if penetration into the perivitelline space could occur. This study found that gorilla sperm bound well to both gorilla and human hemizonae, with a mean of 112.5 and 81.0 tightly bound sperm, respectively. Human sperm also bound to gorilla (mean 229.5) and human (mean 236.5) hemizonae. Following incubation with intact gorilla zonae, motile human sperm were found within the perivitelline space. However, gorilla sperm were not visible within the perivitelline space of nonviable human oocytes. These findings demonstrate that the zonae of nonviable human oocytes can be used to assess sperm binding of gorilla sperm. Studies continue for optimizing assay condition and correlation of findings with the fertility potential of gorilla sperm.

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Section: Introductionmentioning

confidence: 99%

Hemizona assay for measuring zona binding in the lowland gorilla

Lanzendorf

Holmgren

Johnson

et al. 1992

Molecular Reproduction Devel

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“…We describe the techniques used to produce the first gorilla infant from IVF/ ET. Previous studies have demonstrated that human-assisted reproduction techniques can be adapted for ovarian stimulation, oocyte retrieval, and in vitro fertilization in the gorilla [Burton and Ramsey, 1986;Loskutoff et al, 1991a,b;Lazendorf et al, 1992;Yee et al, 1990]. However, the animals available in the pilot studies were older, nonreproductive females with physiological limitations that compromised the likelihood of in vitro embryo production.…”

Section: Discussionmentioning

confidence: 99%

Birth of a Western Lowland gorilla (Gorilla gorilla gorilla) following in vitro fertilization and embryo transfer

et al. 1997

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A 21‐year‐old multiparous female exhibiting 31–41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocyctes (n = 11) were placed in modified human tubal fluid (mHTF, 100 μl) medium under oil at 37°C in humidified 5% CO2. Frozen semen, collected by voluntary ejaculation, was thawed (70°C H2O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two‐cell stage, five were cryopreserved, and three were cultured to the six‐ to eight‐cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247–260, 1997. © 1997 Wiley‐Liss, Inc.

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“…If indications of incipient OHSS were noted during the monitoring sessions, the cycle was to be canceled by withholding further hMG/hCG. Although laparoscopic oocyte retrievals are feasible (3,4), for unknown reasons, dense intraabdominal adhesions appear to be common in captive lowland gorillas (15) and may restrict laparoscopic visualization of the ovaries. Adhesions may also retain the ovaries high in the pelvis, making them inaccessible to vaginal ultrasonography.…”

Section: Discussionmentioning

confidence: 99%

“…In a preliminary study, the addition of a long-acting GnRHa, leuprolide acetate (Lupron Depot; TAP Pharmaceuticals, North Chicago, IL) avoided premature luteinization (3). However, hMG administration required a longer period of time (Table II).…”

Section: Review Of Previous Attemptsmentioning

confidence: 99%