Strategies for ovulation induction and oocyte retrieval in the lowland gorilla

Hatasaka, Harry H.; Schaffer, N.; Chenette, Phillip E.; Kowalski, Wlodzimierz; Hecht, Bryan R.; Meehan, Thomas P.; Wentz, Anne Colston; Valle, Rafael F.; Chatterton, Robert T.; Jeyendran, Rajasingam S.

doi:10.1007/bf02765779

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Here, because of many similarities between spiny mouse and human reproduction, we have developed a novel superovulation and embryo transfer protocol for A. cahirinus based on a human agonist cycle. Although GnRH agonists have been used in superovulation protocols for other menstruating species [33][34][35][36] such as baboons [35] and cynomolgus monkeys [33][34][35][36] they were injected, not implanted, with the GnRH agonists. In our experience, simultaneous hormone and agonist injections are likely to cause excessive, investigator-induced handling stress [37] and negatively affect superovulation outcomes.…”

Section: Discussionmentioning

confidence: 99%

A human-based assisted reproduction protocol for the menstruating spiny mouse, Acomys cahirinus

et al. 2020

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The Egyptian or Common spiny mouse (A. cahirinus) is the first rodent species to show human-like menstruation and spontaneous decidualisation. We consider from these, and its other, human-like characteristics that this species will be a more useful and appropriate small animal model for human reproductive studies. Based on this, there is a need to develop specific laboratory-based assisted reproduction protocols including superovulation, in-vitro fertilisation, embryo cryopreservation and transfer to expand and make this model more relevant. Because standard rodent superovulation has not been successful in the spiny mouse, we have selected to test a human protocol. Female spiny mice will receive a subcutaneous GnRH agonist implant and be allowed to recover. Menstrual cycle lengths will then be allowed to stabilize prior to ovarian stimulation. After recovery, females will be injected IP once a day for 4 days with a FSH analogue, to induce follicular growth, and on day 5 will be injected IP with a hCG analogue to trigger ovulation. Females will either be culled 36hrs after trigger to collect oocytes or immediately paired with a stud male and two cell embryos collected 48hrs later. Mature oocytes will be inseminated using fresh spiny mouse spermatozoa and all in-vitro grown and in-vivo collected two cell embryos will be cryopreserved using methods developed in a close spiny mouse relative, the Mongolian gerbil. For embryo transfer, vitrified embryos will be rapidly warmed and non-surgically transferred to surrogate mice. Surrogates will be monitored until pregnancy is apparent (roughly 30 days) and then left undisturbed until birth, 38–40 days after transfer. By successfully developing robust assisted reproduction protocols in A. cahirinus we will be able to use this rodent as a more effective model for human reproduction.

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Section: Discussionmentioning

confidence: 99%

A human-based assisted reproduction protocol for the menstruating spiny mouse, Acomys cahirinus

et al. 2020

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“…Of these 82 individuals, 49 (60%) have no living offspring, and 43 (52%) are actual or potential founder animals [Wharton, 2000]. Much effort has focused on the development of assisted reproductive technology (ART) for the gorilla, including semen collection and preservation, artificial insemination (AI), ovarian superovulation, oocyte collection by transvaginal ultrasound-guided aspiration, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and embryo transfer [Barrie et al, 1993;Douglass & Gould, 1981;Gould, 1990;Hatasaka et al, 1997;Huntress et al, 1989;Lambert et al, 1991;Lanzendorf et al, 1992;Loskutoff et al, 1991;Platz et al, 1980;Pope et al, 1997;Schaffer et al, 1991;Tribe et al, 1989]. In situations where natural breeding has been unsuccessful, ART may become necessary for the reproduction of genetically underrepresented individuals.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometric Sorting of Fresh and Frozen‐Thawed Spermatozoa in the Western Lowland Gorilla (Gorilla gorilla gorilla)

O’Brien

Stojanov

Crichton³

et al. 2005

American J Primatol

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We adapted flow cytometry technology for high-purity sorting of X chromosome-bearing spermatozoa in the western lowland gorilla (Gorilla gorilla gorilla). Our objectives were to develop methodologies for liquid storage of semen prior to sorting, sorting of liquid-stored and frozenthawed spermatozoa, and assessment of sorting accuracy. In study 1, the in vitro sperm characteristics of gorilla ejaculates from one male were unchanged (P40.05) after 8 hr of liquid storage at 151C in a non-egg yolk diluent (HEPES-buffered modified Tyrode's medium). In study 2, we examined the efficacy of sorting fresh and frozen-thawed spermatozoa using human spermatozoa as a model for gorilla spermatozoa. Ejaculates from one male were split into fresh and frozen aliquots. X-enriched samples derived from both fresh and frozen-thawed human semen were of high purity, as determined by fluorescence in situ hybridization (FISH; 90.7%72.3%, overall), and contained a high proportion of morphologically normal spermatozoa (86.0%71.0%, overall). In study 3, we processed liquid-stored semen from two gorillas for sorting using a modification of methods for human spermatozoa. The sort rate for enrichment of X-bearing spermatozoa was 7.372.5 spermatozoa per second. The X-enriched samples were of high purity (single-sperm PCR: 83.7%) and normal morphology (79.0%73.9%). In study 4 we examined frozen-thawed gorilla semen, and the sort rate (8.372.9 X-bearing sperm/ sec), purity (89.7%), and normal morphology (81.4%73.4%) were comparable to those of liquid-stored semen. Depending on the male and the type of sample used (fresh or frozen-thawed), 0.8-2.2% of gorilla 66:297-315 (2005) spermatozoa in the processed ejaculate were present in the X-enriched sample. These results demonstrate that fresh or frozen-thawed gorilla spermatozoa can be flow cytometrically sorted into samples enriched for X-bearing spermatozoa. Am. J. Primatol. 66:297-315, 2005. r 2005 Wiley-Liss, Inc.

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“…As a pre-requisite for the realization of this idea, we need to obtain large numbers of oocytes from chimpanzees. Ovarian stimulation with exogenous gonadotropins for multiple follicular development has become a common technique of reproductive medicine in humans, and has also been reported in primates including rhesus monkeys [46], cynomolgus monkeys [13], squirrel monkeys [7], marmosets [22], lowland gorillas [15] and chimpanzees [14,45].…”

Section: Introductionmentioning

confidence: 99%

Ovarian follicular development stimulated by leuprorelin acetate plus human menopausal gonadotropin in chimpanzees

Yoshimoto¹,

Shimoda²,

Mori³

et al. 2005

J of Medical Primatology

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We attempted ovarian stimulation using gonadotropins in 14 chimpanzees. Subjects were given a single administration of leuprorelin acetate, followed by repeated administration of human menopausal gonadotropin (hMG) for 16-21 days. During the dosing period, the ovarian follicle diameter and count were measured by transvaginal ultrasonography. The hormone administration induced the development of multiple follicles, and multiple oocytes were subsequently retrieved. However, the follicle count was decreased, suggesting atresia, in some subjects. Statistically, the final follicle diameter was dependent on the dosing duration and the hMG dose in the late stage, while the maximum follicle count during hMG administration was dependent on age and the hMG dose in the early stage. Five subjects showed mild ovarian hyperstimulation syndrome (OHSS)-like symptoms with a high serum estradiol (E2) concentration. These results suggest that leuprorelin acetate plus hMG administration successfully stimulates the development of multiple ovarian follicles for oocyte retrieval and that the serum E2 concentration is predictive of OHSS-like symptoms in chimpanzees.

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Strategies for ovulation induction and oocyte retrieval in the lowland gorilla

Abstract: Purpose: Ovulation induction and oocyte retrieval were performed in a lowland gorilla in an attempt

Cited by 6 publications

References 21 publications

A human-based assisted reproduction protocol for the menstruating spiny mouse, Acomys cahirinus

A human-based assisted reproduction protocol for the menstruating spiny mouse, Acomys cahirinus

Flow Cytometric Sorting of Fresh and Frozen‐Thawed Spermatozoa in the Western Lowland Gorilla (Gorilla gorilla gorilla)

Ovarian follicular development stimulated by leuprorelin acetate plus human menopausal gonadotropin in chimpanzees

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