A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia

Abstract

Early detection of the most common pediatric neoplasm, B-cell precursor lymphoblastic leukemia (BCP-ALL), is challenging and requires invasive bone marrow biopsies. The purpose of this study was to establish new biomarkers for early screening to detect pediatric leukemia. In this small cohort study, Fourier transform infrared (FTIR) spectra were obtained from blood sera of 10 patients with BCP-ALL and were compared with the control samples from 10 children with some conditions other than neoplasm. Using various analytical approaches, including a new physical model, some significant differences were observable. The most important include: the different peak area ratio 2965/1645 cm−1 (p = 0.002); the lower average percentage of both β-sheet and β-turn protein structures in the sera of BCP-ALL patients (p = 0.03); an AdaBoost-based predictive model for classifying healthy vs. BCP-ALL patients with 85% accuracy; and the phase shift of the first derivative in the spectral range 1050–1042 cm−1 correlating with white blood cell (WBC) and blast cell count in BCP-ALL patients contrary to the samples obtained from healthy controls. Although verification in larger groups of patients will be necessary, these promising results suggest that FTIR spectroscopy may have future potential for the early screening of BCP-ALL.

Synchrotron Fourier Transform Infrared Microscopy Spectra in Cellular Effects of Janus Kinase Inhibitors on Myelofibrosis Cancer Cells

“…The control group peak area at 1241 cm –1 was identified as the asymmetric/symmetric stretching of PO 2– (nucleic acids, phosphorylated proteins, and phospholipids). 19 This correlates with the peak result of the treated cells, which exhibited high synthesized nucleic acid levels at 1243–1238 cm –1 . As a result of both type I inhibitor effect mechanisms, Ruxolitinib decreased signaling can be associated with the accumulation of activation loop phosphorylation for preventing JAK2 dephosphorylation and ubiquitination.…”

Fourier Transform Infrared (FTIR) Spectroscopy to Analyse Human Blood over the Last 20 Years: A Review towards Lab-on-a-Chip Devices

“…The frequencies of t amide I band are originally fixed to the secondary structure of the proteins. The positi of the amide I band was at 1645 cm −1 in the FTIR spectrum of the control group, where for the BCP-ALL patients, the peak was shifted to 1641 cm −1 [42], as seen in Figure 9. Thus, the differences between the FTIR spectral profile of leukemic and normal serum may offer a potential route to the early identification of children with BCP-ALL, limiting the number of invasive procedures and accelerating the diagnosis of individuals.…”

“…The frequencies of the amide I band are originally fixed to the secondary structure of the proteins. The position of the amide I band was at 1645 cm −1 in the FTIR spectrum of the control group, whereas for the BCP-ALL patients, the peak was shifted to 1641 cm −1 [42], as seen in Figure 9. This new method presents a reagent-free alternative to traditional colourimetric pr tein determination assays and requires no special sample preparation to investigate E [41].…”

“…FTIR was also used to detect biomarkers for early screening of pediatric leukaemia [42]. In the reported study, the spectra were acquired from blood serum samples of ten child patients with B-cell precursor lymphoblastic leukaemia (BCP-ALL) and were contrasted with ten control samples.…”

Evaluation by MALDI-TOF MS and PCA of the diversity of biosurfactants and their producing bacteria, as adaption to weathered oil components

“…6 B). These shifts are attributed to changes in protein secondary structure [ 54 , 55 ]. Moreover, the peaks at 1154 cm −1 that may be ascribed presence of ester bonds [56] are characteristics of the biosurfactants categorized in PCA-Group 2 ( Fig.…”