A Preliminary Investigation into the Impact of a Pesticide Combination on Human Neuronal and Glial Cell Lines In Vitro

Coleman, Michael D.; O’Neil, John D.; Woehrling, Elizabeth K.; Ndunge, Oscar Bate Akide; Hill, Eric J.; Menache, André; Reiss, Claude

doi:10.1371/journal.pone.0042768

Cited by 46 publications

(42 citation statements)

References 57 publications

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“…Results from these viability experiments showed that neurons are more sensitive to cytotoxic effects of agents tested in all cases excepting for GF, which induces similar cytotoxicity in both cell types. This agrees with previous studies generally showing more sensitivity of neuronal cells to cytotoxicity induction as compared to glial cells (Coccini et al, 2015;Coleman et al, 2002;Rovetta et al, 2007). The relatively higher resistance of A172 cells to toxicity compared with the SH-SY5Y cells reflects to some extent the role of astrocytes in vivo.…”

Section: Discussionsupporting

confidence: 92%

“…However, since they have different purpose in the organism, they are expected to respond to neurotoxic insults in a disparate way, making it difficult to establish the neurotoxic risk of one specific agent. Indeed, the diverse response of these cells against the same external insult (Coccini et al, 2015;Coleman et al, 2002), and also the different effects manifested regarding cells from other origins (e.g., hepatocytes, kidney cells, lymphocytes, skeletal muscle cells, etc.) (Rovetta et al, 2007;Valdiglesias et al, 2010) have been previously documented, supporting the relevance of employing particularly nervous system cell types for testing the toxicity of chemicals suspicious of being able to cross the bloodbrain barrier (e.g., nanomaterials).…”

Section: Discussionmentioning

confidence: 99%

“…SH-SY5Y is a neuroblastoma cell line extensively used as an in vitro neuronal model for the exploration of toxic cellular mechanisms and neurotoxin screening (Coleman et al, 2002). These neuronal cells present the capability of proliferating in culture for long periods without contamination, a prerequisite for the development of an in vitro cell model, and possess many biochemical and functional features of neurons even though, due to their immature neoplastic neural crest origin, they preserve properties of stem cells (Xie et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing

Laffón

Fernández-Bertólez

Costa

et al. 2017

Food and Chemical Toxicology

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a b s t r a c tCell cultures from neuronal and glial origin have proven to be powerful tools for elucidating cellular and molecular mechanisms of nervous system development and physiology, and as neurotoxicity models to evaluate in vitro the possible effects of chemicals. But cellular heterogeneity of nervous system is considerable and these cells have been shown to respond diversely to neurotoxic insults, leading to disparate results from different studies. To shed more light on suitability of cellular models of nervous origin for neurotoxicity screening, the objective of this study was to compare the sensitivity to genetic damage induction of two nervous cell lines. To this aim, neurons (SH-SY5Y) and glial (A172) cells were treated with differently-acting genotoxic agents (bleomycin, actinomycin-D, methyl methanesulfonate, mitomycin C, and griseofulvin). After discarding cytotoxicity, genotoxicity was evaluated by a battery of assays encompassing detection of different genetic lesions. Results obtained showed that glial cells are generally more resistant to genotoxic damage induced by clastogenic agents, but more sensitive to aneugenic effects. These results highlight the need of proper design of in vitro neurotoxicology studies, especially for neurogenotoxicity screening, emphasizing the importance of employing more than one nervous cell type for testing the potential toxicity of a particular exposure.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing

Laffón

Fernández-Bertólez

Costa

et al. 2017

Food and Chemical Toxicology

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“…Cells were then stimulated by experimental treatment medium for 48 h. The cells were washed twice with serumfree medium and incubated with a final concentration of 1 µM JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide) in DMEM medium for 30 min at 37˚C and 5% CO 2 (17,18). After the medium was removed, each treatment group of cells was washed with PBS 3 times, and 100 µl of PBS was added to each well.…”

Section: Mitochondrial Membrane Potential Detection By Jc-1 Stainingmentioning

confidence: 99%

Non-essential amino acids attenuate apoptosis of gastric cancer cells induced by glucose starvation

et al. 2014

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Abstract. Energy and nutrition are essential requirements for all living cells, including cancer cells. In the initiating stage of cancer in organs, cancer cells grow fast and have inadequate supplies of glucose, oxygen and other nutrients due to deficient angiogenesis. Anaerobic conditions cause cancer cells to rely on glycolysis, which produces pyruvate and ATP that can be used by cancer cells to survive. However, glucose starvation may result in apoptosis or necrosis of cancer cells. It has been reported that autophagy is a consequence of glucose starvation and that amino acids are products of autophagy. The present study investigated whether amino acids may represent an alternative energy source for cancer cells undergoing glucose starvation. With non-essential amino acids, growth inhibition and apoptosis of gastric cancer cells induced by glucose starvation were attenuated compared with that of cells undergoing glucose starvation without amino acids, as measured by cell viability, apoptosis rates, membrane potential of mitochondria, and apoptosis-related genes. Meanwhile, both mitochondrial DNA copy number and amino acid transporter genes were increased compared with those in control cells. Non-essential amino acids prevented gastric cancer cells from glucose starvation-induced apoptosis.

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“…To follow the effect of pesticide poisioning in human body can cause a decrease of cholinesterase levels in the blood. [25], [26].…”

mentioning

confidence: 99%

Safety Talk and Check to Prevent Pesticide Toxicity among Farmer

Mahyuni¹,

Yustina²,

Sudaryati³

2017

IJPHS

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The problem at informal sector in particular for farmer when used the pesticides. In Sumber Mufakat village the farmers always use the pesticides without using the safety equipment and have direct contact with the pesticides exposure. The purpose of this research was to apply the safety talk and check method as prevention method to solve the pesticides toxicity that asses from safety and health of pesticide use behaviour. This preventive intervention research was using Participatory Action Research (PAR) design.The data collected by observation, quetionaire, and health check list. The population was all holticultura farmers in Sumber Mufakat village that divide in nine farmer's group. The sample collected by using proportional random sampling. The data will analyze by using qualitative descriptive. The result showed that the implication of safety talk and check method could prevent of pesticide exposure that present from safety and health of pesticides use behaviour. To support the action of safety talk method needed to develop one community that care about the farmers with government endorsement. The check method was very helpful the farmers to identify and predict their health. Both of it could be to cultivate the farmer in safety of pesticide used and early discovery of health symptom cause pesticide used.

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A Preliminary Investigation into the Impact of a Pesticide Combination on Human Neuronal and Glial Cell Lines In Vitro

Cited by 46 publications

References 57 publications

Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing

Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing

Non-essential amino acids attenuate apoptosis of gastric cancer cells induced by glucose starvation

Safety Talk and Check to Prevent Pesticide Toxicity among Farmer

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