A PPARγ, NF-κB and AMPK-Dependent Mechanism May Be Involved in the Beneficial Effects of Curcumin in the Diabetic db/db Mice Liver

Jiménez-Flores, Lizbeth M; López-Briones, Sergio; Macías-Cervantes, Maciste Habacuc; Ramı́rez-Emiliano, Joel; Pérez-Vázquez, Victoriano

doi:10.3390/molecules19068289

Cited by 127 publications

(97 citation statements)

References 56 publications

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“…Multiple mechanisms, like decreased macrophage accumulation in WAT, down-regulation of proinflammatory cytokines and increase in adiponectin levels, are exhibited by curcumin for improving insulin sensitivity [29]. Curcumin increased the expression of AMPK in diabetic db/db mice, which might be a possible mechanism in suppressing hepatic glucose production, resulting in decreased blood glucose levels [30]. Curcumin exhibits cytoprotective mechanisms increasing levels of key mitochondrial biogenesis-regulating transcription factors like PGC-1α, nuclear respiratory factor-1 and mitochondrial transcription factor A [31].…”

Section: Discussionmentioning

confidence: 99%

Curcumin and long-chain Omega-3 polyunsaturated fatty acids for Prevention of type 2 Diabetes (COP-D): study protocol for a randomised controlled trial

Thota

Acharya

Abbott

et al. 2016

Trials

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BackgroundLifestyle interventions, including increase in physical activity and dietary counselling, have shown the ability to prevent type 2 diabetes (T2D) in high-risk state individuals, but the prevalence is still skyrocketing in Australia, in line with global prevalence. Currently, no medicines are approved by the Therapeutic Goods Administration in Australia for the management of prediabetes. Therefore, there is a need of developing a safer, biologically efficacious and cost-effective alternative for delaying the transition of individual health state from prediabetes into T2D. In the current trial we propose to evaluate the effects of curcumin and/or long-chain omega-3 polyunsaturated fatty acids on improving glycosylated haemoglobin as a primary outcome, along with secondary outcomes of glycaemic indices, lipid profile and inflammatory parameters.Methods/designEighty individuals diagnosed with prediabetes, aged between 30 and 70 years, will be randomly assigned to double placebo, curcumin alone, fish oil alone or double active groups according to a computer-generated randomisation sequence for 12 weeks. At baseline and post-intervention visits participants will be asked to provide blood samples and undergo body composition measurements. A blood sample is used for estimating glycaemic profiles, lipid profiles and inflammatory parameters (C-reactive protein, whole blood cell count, adiponectin, leptin, interleukin-6). The interim visit includes review on compliance with supplements based on capsule log and capsule count, adverse events and anthropometric measurements. In addition to these procedures, participants provide self-reported questionnaires on dietary intake (using a 3-day food record), a physical activity questionnaire and medical history.DiscussionThis trial aims to determine whether curcumin and/or long-chain omega-3 polyunsaturated fatty acids affect surrogate markers of glycaemic control which is relevant to delaying T2D. To date 38 participants completed the trial. No changes have been made to the clinical protocol post recruitment. If successful, this trial will provide considerable evidence for performing a larger trial to investigate whether this combination can be administered for preventing or delaying the onset of T2D in high-risk individuals.Trial registration ACTRN12615000559516, registered on 29 May 2015).Electronic supplementary materialThe online version of this article (doi:10.1186/s13063-016-1702-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Curcumin and long-chain Omega-3 polyunsaturated fatty acids for Prevention of type 2 Diabetes (COP-D): study protocol for a randomised controlled trial

Thota

Acharya

Abbott

et al. 2016

Trials

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“…In short, this molecule has a beneficial effect for treatment of diabetic complications via the regulation of the expression of AMPK, PPARγ, and NF-κB (Jimenez-Flores et al, 2014). In another study, curcuminoids have been reported to induce the modulatory subunit of gamma-glutamyl-cysteine ligase; expression of heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 at both the mRNA and protein levels in human islets.…”

Section: Effect Of Curcumin On Pancreaticߚ-cell Dysfunctionmentioning

confidence: 95%

The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update

Ghosh

Banerjee

Sil

2015

Food and Chemical Toxicology

435

250

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“…SDS-PAGE and western blot were performed as previously described [12]: 30 μg of placenta lysate was separated on 10% polyacrylamide gel, and resolved proteins were transferred to nitrocellulose membrane. Molecular weights were identified by comparison with the motility of pre-stained protein standards (Precision Plus Protein Standards, Bio-Rad Laboratories).…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial content, oxidative, and nitrosative stress in human full-term placentas with gestational diabetes mellitus

Ramı́rez-Emiliano

Fajardo-Araujo

Zúñiga-Trujillo

et al. 2017

Reprod Biol Endocrinol

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BackgroundThe purpose of this study was to determine the mitochondrial content, and the oxidative and nitrosative stress of the placenta in women with gestational diabetes mellitus (GDM).MethodsFull-term placentas from GDM and healthy pregnancies were collected following informed consent. The lipid peroxidation (TBARS) and oxidized protein (carbonyls) levels were determined by spectrophotometry, and 3-nitrotyrosine (3-NT), subunit IV of cytochrome oxidase (COX4), adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK) and actin were determined by western blot, whereas ATPase activity was performed by determining the adenosine triphosphate (ATP) consumption using a High-performance liquid chromatography (HPLC) system.ResultsTBARS and carbonyls levels were lower in the placentas of women with GDM compared with the normal placentas (p < 0.001 and p < 0.05, respectively). Also, 3-NT/actin and AMPK/actin ratios were higher in GDM placentas than in the normal placentas (p = 0.03 and p = 0.012, respectively). Whereas COX4/actin ratio and ATPase activity were similar between GDM placentas and those controls.ConclusionsThese data suggest that placentas with GDM are more protected against oxidative damage, but are more susceptible to nitrosative damage as compared to normal placentas. Moreover, the increased expression levels of AMPK in GDM placentas suggest that AMPK might have a role in maintaining the mitochondrial biogenesis at normal levels.Trial registration HGRL28072011. Registered 28 July 2011.

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A PPARγ, NF-κB and AMPK-Dependent Mechanism May Be Involved in the Beneficial Effects of Curcumin in the Diabetic db/db Mice Liver

Cited by 127 publications

References 56 publications

Curcumin and long-chain Omega-3 polyunsaturated fatty acids for Prevention of type 2 Diabetes (COP-D): study protocol for a randomised controlled trial

Curcumin and long-chain Omega-3 polyunsaturated fatty acids for Prevention of type 2 Diabetes (COP-D): study protocol for a randomised controlled trial

The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update

Mitochondrial content, oxidative, and nitrosative stress in human full-term placentas with gestational diabetes mellitus

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