A potentiometric protein sensor built with surface molecular imprinting method

Wang, Yantian; Zhou, Yihang; Sokolov, J.; Rigas, Basil; Levón, Kalle; Rafailovich, Miriam

doi:10.1016/j.bios.2008.04.010

Cited by 117 publications

(60 citation statements)

References 32 publications

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“…Binding of charged protein to an insulating layer of MIP over an electrode alters the surface potential, allowing binding to be measured potentiometrically. 164 The sensor response depends on the isoelectric point of the protein and the pH of the sample matrix, however this dependency may be exploited to enhance specificity.…”

Section: Electrical and Electrochemical Sensorsmentioning

confidence: 99%

The rational development of molecularly imprinted polymer-based sensors for protein detection

et al. 2011

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The detection of specific proteins, as biomarkers of disease, health status, environmental monitoring, food quality, control of fermenters and civil defence purposes means that biosensors for these targets will become increasing more important. Among the technologies used for building specific recognition properties, molecularly imprinted polymers, molecularly imprinted polymers (MIPs) are attracting much attention. In this critical review we describe many of methods used for imprinting recognition for protein targets in polymers and their incorporation with a number of transducer platforms with the aim of identifying the most promising approaches for the preparation of MIP-based protein sensors.2

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Section: Electrical and Electrochemical Sensorsmentioning

confidence: 99%

The rational development of molecularly imprinted polymer-based sensors for protein detection

et al. 2011

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“…So far, different strategies for Myo detection and quantification based on surface imprinting were described in the literature, including sol-gel methods merged with self assembling monolayer for potentiometric transduction [6,22], silicon surface grafting [13] and thin film/micro contact imprinting mass with spectrometrybased profiling system [23], none of these coupling these two simple concepts of MIP receptors and SPE technology. Several techniques have been described in the literature for Myo quantification [24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor based on biomimetic material for myoglobin detection

Moreira

Dutra

Noronha

et al. 2013

Electrochimica Acta

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a b s t r a c tA novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification.The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 g mL −1 , of positive slope 6.50 ± 1.48 (k mL g −1 ). The limit of detection was 2.25 g mL −1 . Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 g mL −1 . A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 A mL g −1 . MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.

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“…It is often associated with (i) irregular shape of the imprinted particles, (ii) poor template removal by protein entrapment and (iii) restricted stereochemical access of the protein to the rebinding sites (Whitcombe et al, 2011;Wang et al, 2008). Epitope imprinting seems to be a successful strategy (Whitcombe et al, 2011;Hillberg and Tabrizian, 2008), but only small to medium sized peptides have been imprinted so far and further studies are required to obtain unique and freely available epitope sequences (Hillberg and Tabrizian, 2008;Whitcombe et al, 2011).…”

Section: Cardiac)mentioning

confidence: 99%

“…imprinting, in which the whole protein is imprinted (Katz and Davis, 2000;Savina et al, 2009;Ye and Mosbach, 2001); (ii) epitope imprinting, where only the antigenic determinant part of the protein is used for imprinting (Bossi et al, 2012;LassNapiorkowska et al, 2012;Lu et al, 2012) and (iii) surface imprinting in which only a part of the surface of the protein is imprinted over a planar surface (Moreira et al 2011a;Moreira et al, 2011b;Wang et al, 2008Yildirim et al, 2012Liao et al, 2009).…”

Section: Cardiac)mentioning

confidence: 99%

Smart plastic antibody material (SPAM) tailored on disposable screen printed electrodes for protein recognition: Application to myoglobin detection

Moreira

Sharma

Dutra

et al. 2013

Biosensors and Bioelectronics

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This work introduces two major changes to the conventional protocol for designing plastic antibodies: (i) the imprinted sites were created with charged monomers while the surrounding environment was tailored using neutral material; and (ii) the protein was removed from its imprinted site by means of a protease, aiming at preserving the polymeric network of the plastic antibody. To our knowledge, these approaches were never presented before and the resulting material was named here as smart plastic antibody material (SPAM).As proof of concept, SPAM was tailored on top of disposable gold -screen printed electrodes (Au-SPE), following a bottom-up approach, for targeting myoglobin (Myo) in a point-of-care context. The existence of imprinted sites was checked by comparing a SPAM modified surface to a negative control, consisting of similar material where the template was omitted from the procedure and called non-imprinted materials (NIMs). All stages of the creation of the SPAM and NIM on the Au layer were followed by both electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). AFM imaging was also performed to characterize the topography of the surface.There are two major reasons supporting the fact that plastic antibodies were effectively designed by the above approach: (i) they were visualized for the first time by AFM, being present only in the SPAM network; and (ii) only the SPAM material was able to rebind to the target protein and produce a linear electrical response against EIS and square wave voltammetry (SWV) assays, with NIMs showing a similar-to-random behavior. The SPAM/Au-SPE devices displayed linear responses to Myo in EIS and SWV assays down to 3.5 mg/mL and 0.58 mg/mL, respectively, with detection limits of 1.5 and 0.28 mg/mL.SPAM materials also showed negligible interference from troponin T (TnT), bovine serum albumin (BSA) and urea under SWV assay s, showing promising results for point-of-care applications when applied to spiked biological fluids.

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A potentiometric protein sensor built with surface molecular imprinting method

Cited by 117 publications

References 32 publications

The rational development of molecularly imprinted polymer-based sensors for protein detection

The rational development of molecularly imprinted polymer-based sensors for protein detection

Electrochemical biosensor based on biomimetic material for myoglobin detection

Smart plastic antibody material (SPAM) tailored on disposable screen printed electrodes for protein recognition: Application to myoglobin detection

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