A Potential New Gene (tccA) on IncP Plasmid RK2 and Transposon Tn1721:Relationship of Its Product to the TrwC Relaxase/Helicase of IncW Plasmid R388

Jovanovic, Oliver S.; Figurski, David H.

doi:10.1006/plas.1997.1314

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…This fragment was shotgun cloned from plasmid pFBAOT6 into pUC18, and sequencing was carried out from each end of the cloned insert such that partial sequences of 647 and 620 bp were obtained. The first 647 bp of one end of the insert shared 95% homology with Tn1721 (encompassing the first right inverted repeat (IRR-I), the tccA gene (27), and part of the tetR gene, respectively). The first 620 bp of the opposite end of the insert shared 95% homology with IRR-II and part of the tnpAЈ region of Tn1721 (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Rhodes

Huys

Swings

et al. 2000

Appl Environ Microbiol

349

262

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Oxytetracycline-resistant (OT r ) mesophilic aeromonads were recovered from untreated hospital effluent (72 isolates) and from fish farm hatchery tanks (91 isolates) at sites within the English Lake District, Cumbria, England. The transfer of OT r plasmids from these isolates was investigated. Using Escherichia coli J53-1 as a recipient, 11 isolates from the hospital site and 6 isolates from the fish farm site transferred OT r plasmids (designated pFBAOT1 to 17). Original isolates were identified using fatty acid methyl ester and fluorescent amplified fragment length polymorphism comparisons as either Aeromonas hydrophila HG3 (eight isolates), A. veronii b.v. sobria HG8 (six isolates), and A. caviae HGB5 (one isolate). One isolate remained unidentified, and one could not be assigned a taxonomic designation beyond the genus level. Plasmids pFBAOT1 to -17 were screened for the presence of the tetracycline resistance determinants Tet A to E and Tet G. Only determinant Tet A (10 plasmids) was detected in these plasmids, with 7 tet gene determinants remaining unclassified. In all cases, Tet A was located on a 5.5-kb EcoRI restriction fragment. Hybridization with inc-rep probes N, P, Q, W, and U showed pFBAOT3, -4, -5, -6, -7, -9, and -11, from the hospital environment, to be IncU plasmids. Further, restriction fragment length polymorphism (RFLP) analyses and DNA probing demonstrated that pFBAOT plasmids were closely related to IncU OT r plasmids pASOT, pASOT2, pASOT3, pRAS1 (originally isolated from A. salmonicida strains from fish farms in Scotland and Norway, respectively), and pIE420 (isolated from a German hospital E. coli strain). In addition, DNA analyses demonstrated that plasmids pRAS1 and pIE420 had identical RFLP profiles and that all fragments hybridized to each other. The presence of tetracycline resistance transposon Tn1721 in its entirety or in a truncated form in these plasmids was demonstrated. These results provided direct evidence that related tetracycline resistance-encoding plasmids have disseminated between different Aeromonas species and E. coli and between the human and aquaculture environments in distinct geographical locations. Collectively, these findings provide evidence to support the hypothesis that the aquaculture and human compartments of the environment behave as a single interactive compartment.

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Section: Resultsmentioning

confidence: 99%

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Rhodes

Huys

Swings

et al. 2000

Appl Environ Microbiol

349

262

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“…References: IncP-1a and R751 (Pansegrau et al, 1994;Thorsted et al, 1998;Smith & Thomas, 1989;Jovanovic & Figurski, 1997), pB1-pB12 (Droge et al, 2000), pSS50 (Hooper et al,1989), pSS60 (Burlage et al, 1990), pJP4 (Leveau & van der Meer, 1997), R906, R772 , pTSA (Tralau et al, 2001), pADP-1 (Martinez et al, 2001), pPS12-1 (Beil et al, 1999). quite well understood.…”

Section: Replication Determinantsmentioning

confidence: 99%

Spread and survival of promiscuous IncP-1 plasmids.

Adamczyk¹,

Jagura-Burdzy²

2003

Acta Biochim Pol

113

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Plasmids classified to the IncP-1 incompatibility group belong to the most stably maintained mobile elements among low copy number plasmids known to date. The remarkable persistence is achieved by various tightly controlled stability mechanisms like active partitioning, efficient conjugative transfer system, killing of plasmid-free segregants and multimer resolution. The unique feature of IncP-1 plasmids is the central control operon coding for global regulators which control the expression of genes involved in vegetative replication, stable maintenance and conjugative transfer. The multivalent regulatory network provides means for coordinated expression of all plasmid functions. The current state of knowledge about two fully sequenced plasmids RK2 and R751, representatives of the IncP-1alpha and IncP-1beta subgroups, is presented.

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Position effects on promoter activity inEscherichia coliand their consequences for antibiotic-resistance determinants

Cooke

Browning

Lee

et al. 2019

Biochemical Society Transactions

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The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.

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A Potential New Gene (tccA) on IncP Plasmid RK2 and Transposon Tn1721:Relationship of Its Product to the TrwC Relaxase/Helicase of IncW Plasmid R388

Cited by 3 publications

References 16 publications

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Spread and survival of promiscuous IncP-1 plasmids.

Position effects on promoter activity inEscherichia coliand their consequences for antibiotic-resistance determinants

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