A Potential Method for Standardization of Multiphytoadaptogen: Tandem Mass Spectrometry for Analysis of Biologically Active Substances from Rhodiola rosea

Бочарова, О. А.; Казеев, И. В.; Шевченко, В. Е.; Sheichenko, O. P.; Poroikov, Vladimir; Бочаров, Е. В.; Карпова, Р. В.; Ионов, Н. С.; Kucheryanu, V. G.; Косоруков, В. С.; Матвеев, В. Б.; Стилиди, И. С.

doi:10.1007/s11094-022-02607-0

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Recently, due to suppression of postprandial elevation of the blood triglyceride level and owing to their hepatoprotective and prolyl endopeptidase-and neuraminidase-inhibitory effects [43][44][45], the two compounds showed promise as pharmaceuticals or nutritional supplements [46]. Flavonoids may not only contribute to some activities but also may represent an additional analyte for ensuring identity, purity, and consistent composition of medicinal products [5,47]. Regarding R. rosea, the isolated flavonoids are usually glycosides of kaempferol, gossypetin, and herbacetin.…”

Section: The Flavonoid Contentmentioning

confidence: 99%

Individual Differences in Growth and in Accumulation of Secondary Metabolites in Rhodiola rosea Cultivated in Western Siberia

Эрст

Kotsupiy

Erst

et al. 2023

IJMS

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In this study, growth parameters of underground parts and concentrations of phenylpropanoids, phenylethanoids, flavonoids, hydroxybenzoic acids, and catechins in aqueous–ethanol extracts of 6-year-old cultivated plants of Rhodiola rosea (propagated in vitro) of Altai Mountain origin were analyzed, and differences in chemical composition among plant specimens and between plant parts (rhizome and root) were evaluated. High-performance liquid chromatography detected 13 phenolic compounds. Roots contained 1.28 times higher phenylethanoids levels (1273.72 mg/100 g) than rhizomes did. Overall, the concentration of phenylethanoids in underground organs was not high and ranged from 21.36 to 103.00 mg/100 g. High variation among R. rosea individual plants was noted both in growth characteristics and in levels of secondary metabolites under our cultivation conditions. It was found that concentrations of phenylpropanoids, phenylethanoids, and catechins significantly depend on the plant part analyzed (p ≤ 0.05). Specimen No. 4 is characterized by the highest concentration of rosavins (1230.99 mg/plant) and the lowest concentration of cinnamyl alcohol (62.87 mg/plant). Despite the wide range of values, all 10 tested specimens (underground part) met the minimum requirements of the United States Pharmacopeia (2015) for rosavins (0.3%) and of the Russia State Pharmacopoeia (2015) for the average level of rosavins (roots): (1%).

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Section: The Flavonoid Contentmentioning

confidence: 99%

Individual Differences in Growth and in Accumulation of Secondary Metabolites in Rhodiola rosea Cultivated in Western Siberia

Эрст

Kotsupiy

Erst

et al. 2023

IJMS

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“…Высокоэффективной жидкостной хроматографией в сочетании с тандемной масс-спектрометрией (ВЭЖХ-МС / МС) в МФА были идентифицированы основные БАВ женьшеня и аралии -тритерпеновые сапонины (гинзенозиды Rb1, Rb2, Rc, Rd, Rg1, Rg2, Re, Rf, Ro и аралозиды A, В, С) [32,33]. С помощью этой же методологии фенилэтанолгликозид (салидрозид), фенилпропаноидные гликозиды (розавин и розарин), монотерпеновый гликозид (розиридин) и флавоноид (родионин) выявлены как БАВ Rhodiola rosea, а также фенол (элеутерозид B) и лигнан (элеутерозид E) -как БАВ Eleutherococcus senticosus [34,35]. Тритерпеновые гликозиды, идентичные гинзенозидам Re и Rd, а также аралозиду C, были выявлены в МФА как БАВ Oplopanax elatus [36].…”

Section: Introductionunclassified

“…Baill (Schisandraceae), которые транслоцированы в МФА в результате проведения авторской технологии извлечения. Метод ВЭЖХ-МС / МС характеризуется специфичностью и высокой точностью, что позволяет определять вещества в минимальных количествах [32][33][34][35][36].…”

Section: Introductionunclassified

Schisandrin and Schisantherin A in Multiphytoadaptogen for Preventive Oncology

Bocharova,

Kazeev,

Shevchenko

et al. 2023

Rossijskij bioterapevtičeskij žurnal

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Background. The original herbal formula of Multiphytoadaptogen (MPhA) for preventive oncology developed by the N. N. Blokhin Center of Oncology containing phytocomponents from Schisandra chinensis (Turcz.) Baill (Schisandraceae), has been investigated in vitro, in vivo. Preliminary efficiency in clinical trials has also been obtained. This was allowed because MPhA in Russia is registered as a parapharmaceutical agent and therefore standardized according to established requirements. However, due to the high efficiency of MPhA, a detailed study of the chemical composition and standardization of it is required, including the Schisandra chinensis active components, which turned out to be translocated into MPhA as a result of the extraction technology developed.The aim of the study was to identify the Schisandra chinensis biologically active substances in MPhA and to evaluate the biological activity profiles of the identified phytocomponents using in silico analysis.Materials and methods. we used high performance liquid chromatography in combination with mass spectrometry (HPLC–MS / MS). Chromatography was performed on an ACQUITY UPLC BEH C18 column in a gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used. we performed in silico analyzes of Schisandrin and Schisantherin A biological activity spectra using computer programs PASS and PharmaExpert.Result. The secondary metabolites lignans Schisandrin and Schisantherin A were identified in the herbal formula MPhA. Schisandrin and Schisantherin A activities, according the scientific literature and in silico analysis, correspond to the properties studied for MPhA which therefore fits into the concept of medication for preventive oncology.Conclusion. The determined secondary metabolites can be used for identification, standardization and quality testing of the herbal formula MPhA.

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“…Among the components of MPhA, the main BAS in Ginseng and Aralia -triterpene saponins (ginsenosides Rb1, Rb2, Rc, Rd, Rg1, Rg2, Re, Rf, Ro and aralosides A, В, С) were identified by high-performance liquid chromatography in combination with tandem mass spectrometry (HPLC-MS/MS) [33,34]. Phenylethanolglycoside (salidroside), phenylpropanoid glycosides (rosavin and rosarin), monoterpene glycoside (rosiridine) and flavonoid (rhodionine) were also identified as components of Rhodiola rosea BAS as well as phenol (eleutheroside B) and lignan (eleutheroside E) from Eleutherococcus senticosus [35,36]. The compounds identical to ginsenosdes Re and Rd with araloside C were identified in MPhA as components of Oplopánax elátus (triterpene glycosides) [37].…”

Section: Introductionmentioning

confidence: 99%

“…The next stage of the MPhA components analysis using HPLC-MS/ MS is to determine Schisándra chinénsis Baill (Schisandraceae) fruits extract BAS which turned out to be translocated into MPhA as a result of the extraction technology developed. HPLC-MS/ MS method is characterized by specificity and high accuracy, which makes it possible to determine substances in minimal quantities [33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites of Schisandra chinensis in homeostasis regulator adaptogen herbal formula for preventive oncology

Bocharova,

Kazeev,

Shevchenko

et al. 2023

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The original herbal formula of homeostasis regulator Multiphytoadaptogen (MPhA) for preventive oncology developed by the N.N. Blokhin Center of Oncology containing phytocomponents from Schizandra chinensis has been investigated in vitro, in vivo and in clinical studies. The MPhA multi-target effects are achieved by optimizing the functioning of the nervous, immune and endocrine defense systems that regulate homeostasis under stress. Everything that has been previously studied for MPhA can be considered as preclinical testing, including clinical research, which can be regarded as the pilot studies. This was allowed because MPhA in Russia is registered as a parapharmaceutical agent and therefore standardized according to established requirements. However, due to the high efficiency of MPhA, a detailed study of the chemical composition and standardization of it is required, including the composition of Schisandra chinensis Baill (Schisandraceae) active components, which turned out to be translocated into MPhA as a result of the extraction technology developed. So, for MPhA identification and standardization we detected the secondary metabolites in the herbal formula MPhA as well as in fruits extract of Schisándra chinénsis using high-performance liquid chromatography in combination with mass spectrometry. Chromatography was performed on an ACQUITY UPLC BEH C18 column in a gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used. Lignans Schizandrin and Schizantherin A were identified in the MPhA as well as in Schisándra chinénsis fruits extract obtained by the technology developed. The determined secondary metabolites can be used for standardization and quality testing of the herbal formula MPhA. In addition, we performed in silico analyzes of Schizandrin and Schizantherin A biological activity spectra using computer program PASS (Prediction of Activity Spectra for Substances). Schizandrin and Schizantherin A activities, according the scientific literature and in silico analysis, correspond to the properties studied for MPhA which therefore fits into the concept of a drug – homeostasis regulator adaptogen for preventive oncology.

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A Potential Method for Standardization of Multiphytoadaptogen: Tandem Mass Spectrometry for Analysis of Biologically Active Substances from Rhodiola rosea

Cited by 6 publications

References 29 publications

Individual Differences in Growth and in Accumulation of Secondary Metabolites in Rhodiola rosea Cultivated in Western Siberia

Individual Differences in Growth and in Accumulation of Secondary Metabolites in Rhodiola rosea Cultivated in Western Siberia

Schisandrin and Schisantherin A in Multiphytoadaptogen for Preventive Oncology

Secondary metabolites of Schisandra chinensis in homeostasis regulator adaptogen herbal formula for preventive oncology

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