A Potential Biofilm Metabolite Signature for Caries Activity - A Pilot Clinical Study

Abstract

Background This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. Methods Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. Results Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. Conclusions This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.

Antibacterial Activity of Chlorhexidine Bigluconate in Relation to Biofilm Monocultures

“…В немногочисленных публикациях отмечено, что значимых различий антимикробного воздействия 0,5 % и 1 % растворов хлоргексидина на S. аureus и E. coli по зоне задержки роста не установлено [18]. В связи с изложенным выше материалом, очевидно, что определение оптимальной концентрации хлоргексидина биглюконата и экспозиции воздействия на дентин коронковой части зуба при кариозном поражении последнего на текущий момент весьма актуально [19,20,21].…”

Comparison of Medical and Forensic Profiling Potential of Volatile Biomarkers from Different Biological Specimens from Individuals and Across Populations

“…Common names for some of the compounds are given in parentheses and italicized. Many of the presented compounds have been previously reported to be volatile emanation compounds of human skin 5,14,28,29,123 , saliva 127 , breath 44,48,97,128 , volatile constituents of human blood 18,62,66,67 , and urine [70][71][72] . Compounds denoted by "en" are possibly endogenous compounds, while compounds denoted by "ex" are possibly of exogenous origin.…”