A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5

Jong, Walter De; Forsyth, Amanda; Leister, Dario; Gebhardt, Christiane; Baulcombe, David C.

doi:10.1007/s001220050555

Cited by 105 publications

(68 citation statements)

References 32 publications

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“…Described by way of DNA isolation was absolutely relative sufficient to the quantity and quality of DNA in high molecular conditions. Although De Jong et al (1997) and Ballvora et al (2002) described the use of more complicated isolation methods, our methods offered identical results. In comparison to cited authors we had other genotypes for disposal and we did not make any hybridisation experiments.…”

Section: Resultsmentioning

confidence: 83%

“…Markers GP21 and SPUD237 according to De Jong et al (1997) were optimised and used for CAPS analyses. Marker SPUD237 (SPUD237 F 5´ TTC CTG CTG ATA CTG ACT AGA AAA CC 3´, SPUD237 R 5´ AGC CAA GGA AAA GCT AGC ATC CAA G 3´) was amplified under these conditions: 50 ng of total genomic DNA, 2.5mM of MgCl 2 , 0.4mM of each dNTP, 0.5µM of each primer and 0.5 unit of TaqI recombinant DNA polymerase (Fermentas, Lithuania) in 25 µl reaction.…”

Section: Methodsmentioning

confidence: 99%

“…(Bradshaw andMackay 1994, Chloupek 2000). De Jong et al (1997) studied this region by means of AFLP markers and his hybridisation experiments demonstrated a very tight linkage of the R1 gene and marker SPUD237. This marker was converted in CAPS (Cleaved Amplified Polymorphic Sequence) marker more usable for a wider research public.…”

mentioning

confidence: 99%

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Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

Sedlák¹,

Vejl²,

Melounová³

et al. 2005

PLANT SOIL ENVIRON

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Marker assisted selection (MAS) in potato breeding is the most developing area at present time. Methods of DNA markers are developed in all top world potato research institutes and universities oriented on plant production. This paper presents results obtained from the testing of gene resources encompassing different resistance genes against Phytophthora infestans potentially exploitable for Czech potato breeding. Three different DNA markers were studied that are linked to R1 locus in the potato chromosome V operating as a resistance against some races of P. infestans. Markering capability and frequencies of these markers was evaluated with respect to their usage in practical Czech plant breeding that has vital importance in finding the tools as a prerequisite for creating new varieties efficiently.

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Section: Resultsmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

Sedlák¹,

Vejl²,

Melounová³

et al. 2005

PLANT SOIL ENVIRON

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“…Earlier studies suggested that AFLP fragments with lengths between 150-300 bp needed information about the flanking regions for conversion to SCAR markers (De Jong et al 1997;Negi et al 2000). In the present study, the inverse PCR approach was carried out to obtain DNA sequences adjacent to the AFLP marker.…”

Section: Inverse Pcrmentioning

confidence: 97%

Development of a diagnostic DNA marker for the geographic origin of Shorea leprosula

2016

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Abstract:The development of sequence characterized amplified region (SCAR) markers derived from amplified fragment length polymorphisms (AFLPs) is described for Shorea leprosula. An AFLP fragment that showed nearly complete differentiation between Borneo and Sumatra was gel-extracted, sequenced, and converted into a SCAR marker using the inverse polymerase chain reaction (PCR) technique. The single nucleotide polymorphism (SNP) that originally caused the AFLP was found in the MseI restriction site. Differentiation between islands was detected either as size variation of the codominant SCAR marker or after digestion of the PCR products with the restriction enzyme MseI (PCR-RFLP). Size variation was due to insertions/deletions found within the sequenced region that flanked the original AFLP fragment. After genotyping 151 samples of S. leprosula from 14 populations in Sumatra and Borneo, all but one sample from Sumatra were homozygous for one size variant (427 bp), while S. leprosula populations from Borneo showed different genotypes than Sumatra populations and variation not only among populations but also within populations. Complete differentiation and fixation on alternative variants was found for the geographic regions of Sumatra and Borneo by the PCR-RFLP method. The SCAR marker did not amplify in Shorea parvifolia and thus can also be used to distinguish between S. leprosula and S. parvifolia. The marker was successfully amplified from wood DNA extracts suggesting its applicability to track the geographic origin of timber.

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“…Nx phu from S. phureja is located at the distal end of the long arm of chromosome IX (Tommiska et al, 1998). Nb tbr was identified in S. tuberosum and has been localised to chromosome V to the same region that contains Rx2 (De Jong et al, 1997).…”

Section: Genes Associated With Hr Type Resistancementioning

confidence: 99%

Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

Kopp¹,

Kondrák²,

Bánfalvi³

2015

Acta Phytopathologica et Entomologica Hungarica

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The most important viral pathogens of the cultivated potato are Potato virus X (PVX) and Potato virus Y (PVY), which can reduce potato production up to 80%. Thus resistance breeding is one of the major goals of plant breeders. Wild potato species are good sources of resistance (R) genes. The resistant plants respond to viral infection with hypersensitive reaction (HR) or extreme resistance (ER). HR is accompanied by programmed cell death, while ER localizes the virus at the primary infection site and limits virus replication without visible symptoms. While HR is generally strain-specific, ER can act against a broad spectrum of viral pathogens. This review aims to describe the molecular mechanisms of resistance against PVX and PVY in potato.

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A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5

Cited by 105 publications

References 32 publications

Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

Development of a diagnostic DNA marker for the geographic origin of Shorea leprosula

Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

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